COL27A1 (NM_032888) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	STLS
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC305516 representing NM_032888. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGAGCGGGATCGGCGCGGGGGGCCCGAGGCACAGCGGCGGCGGCGGCGGCGCGCGGGGGGGGGTTT CTCTTCTCCTGGATCTTAGTCTCGTTTGCCTGTCACCTGGCCTCCACCCAAGGAGCTCCTGAAGATGTG GACATCCTCCAGCGGCTGGGCCTCAGCTGGACGAAGGCCGGGAGCCCTGCACCCCCGGGAGTCATTCCT TTCCAGTCGGGCTTCATCTTTACGCAGCGGGCCCGGCTCCAGGCTCCCACGGGCACCGTCATTCCTGCC GCCTTGGGCACAGAGCTGGCACTGGTGCTGAGCCTCTGCTCCCACCGGGTGAACCATGCCTTCCTCTTC GCTGTCCGCAGCCAGAAACGCAAGCTGCAGCTGGGCCTGCAGTTCCTCCCCGGCAAGACGGTCGTCCAC CTCGGGTCCCGGCGCTCAGTGGCCTTCGACCTCGACATGCACGACGGGCGCTGGCACCACCTGGCCCTC GAGCTCCGAGGCCGCACAGTCACTCTGGTGACTGCCTGCGGGCAGCGCCGGGTGCCTGTCCTGCTGCCT TTCCACAGGGACCCTGCACTCGACCCTGGGGGCTCCTTCCTCTTTGGGAAGATGAACCCGCATGCAGTC CAGTTTGAAGGTGCTCTCTGCCAGTTCAGTATCTACCCTGTGACGCAGGTCGCTCACAATTACTGTACC CACCTGAGGAAGCAGTGTGGACAGGCTGACACGTACCAGTCCCCACTGGGACCTCTCTTCTCCCAAGAC TCTGGCAGACCTTTTACCTTCCAGTCCGACCTCGCCCTGCTAGGCCTGGAGAACTTGACCACTGCCACA CCAGCCCTGGGGTCACTGCCAGCAGGCAGGGGACCCAGGGGGACTGTGGCACCCGCCACGCCCACCAAG CCCCAAAGGACTAGCCCCACAAACCCTCACCAGCATATGGCGGTGGGAGGCCCAGCCCAAACCCCGCTG CTACCTGCCAAGCTGTCAGCCAGTAACGCACTTGATCCCATGCTCCCAGCCTCTGTTGGCGGCTCTACC AGAACGCCTCGCCCTGCGGCCGCTCAACCATCACAGAAGATCACAGCCACCAAAATCCCCAAAAGCCTC CCTACCAAGCCTTCGGCCCCTTCTACTTCAATTGTGCCCATCAAAAGCCCCCATCCTACCCAGAAAACA GCTCCATCTTCATTTACAAAGTCAGCCCTACCCACTCAGAAGCAAGTGCCACCTACTTCCCGTCCAGTT CCTGCCAGAGTCTCCCGTCCCGCAGAGAAGCCCATCCAGAGGAACCCGGGAATGCCCAGGCCCCCACCG CCCAGCACCCGGCCCCTACCTCCTACCACCAGCTCCTCTAAAAAACCCATTCCCACACTAGCTCGGACT GAGGCCAAGATAACCAGCCATGCCAGTAAGCCGGCCTCTGCCCGCACCAGCACCCACAAACCTCCCCCA TTTACTGCTTTATCCTCATCTCCTGCCCCTACTCCTGGTTCTACCAGGAGTACTCGGCCACCAGCCACG ATGGTACCTCCAACTTCGGGCACCAGCACTCCCAGAACAGCACCTGCCGTCCCCACTCCTGGCTCAGCT CCCACTGGAAGCAAGAAGCCCATTGGATCGGAAGCCTCAAAGAAAGCCGGACCCAAGAGCAGCCCCCGG AAGCCTGTCCCCCTCAGACCTGGGAAGGCAGCCAGGGATGTCCCCTTGAGCGATCTGACAACCAGGCCT AGCCCCAGACAGCCCCAGCCCAGTCAGCAGACCACCCCGGCCCTGGTATTGGCCCCGGCGCAATTCCTG TCCTCCAGCCCCCGGCCCACGAGCAGTGGCTATTCGATCTTCCACCTGGCAGGATCTACGCCTTTCCCT CTGCTGATGGGGCCTCCGGGACCCAAGGGAGACTGTGGCTTGCCGGGTCCCCCTGGGCTACCTGGGCTA CCTGGAATCCCTGGTGCACGTGGGCCTCGGGGTCCTCCTGGGCCTTATGGAAATCCAGGTCTCCCCGGC CCTCCTGGAGCCAAAGGACAGAAAGGGGACCCAGGGCTCTCACCAGGAAAGGCCCACGATGGGGCAAAG GGTGACATGGGCTTGCCTGGGCTCTCCGGGAATCCAGGACCTCCGGGACGAAAGGGACACAAGGGCTAT CCTGGACCGGCAGGGCACCCCGGAGAACAGGGGCAGCCAGGACCTGAGGGCAGCCCAGGGGCCAAAGGT TACCCTGGCAGGCAGGGGTTACCTGGACCGGTAGGAGATCCCGGCCCCAAAGGCAGCAGGGGCTACATT GGGCTCCCAGGGCTCTTCGGCCTGCCAGGGTCTGATGGAGAACGAGGCCTGCCTGGCGTTCCTGGCAAG AGGGGCAAGATGGGTATGCCGGGGTTTCCTGGAGTCTTTGGGGAAAGAGGCCCTCCTGGACTGGATGGA AATCCTGGAGAACTGGGCCTGCCAGGCCCCCCTGGAGTCCCCGGCCTCATTGGTGACTTAGGAGTGTTG GGTCCGATTGGCTACCCGGGACCCAAGGGCATGAAGGGACTGATGGGCAGCGTGGGGGAGCCCGGACTG AAAGGTGATAAGGGTGAACAAGGGGTTCCAGGTGTGTCAGGAGATCCCGGATTCCAAGGAGACAAGGGG AGCCAGGGGTTGCCAGGGTTCCCCGGTGCACGGGGGAAGCCAGGGCCTCTGGGCAAAGTCGGAGACAAA GGATCCATTGGGTTTCCCGGGCCCCCTGGACCCGAGGGATTCCCAGGAGACATCGGCCCCCCTGGCGAC AATGGCCCAGAAGGCATGAAGGGTAAGCCTGGAGCCCGAGGCCTGCCGGGACCCCGTGGGCAGCTGGGG CCCGAGGGAGATGAGGGACCCATGGGGCCGCCAGGGGCCCCTGGCTTGGAGGGTCAGCCTGGCAGGAAG GGGTTTCCTGGGAGGCCCGGCCTGGATGGCGTGAAGGGGGAACCAGGGGATCCTGGTCGGCCGGGGCCT GTGGGAGAGCAGGGATTTATGGGATTCATTGGTCTGGTCGGGGAGCCAGGAATCGTGGGAGAAAAGGGT GATCGTGGCATGATGGGACCCCCAGGCGTGCCTGGACCCAAGGGGTCGATGGGTCATCCTGGAATGCCA GGTGGTATGGGGACCCCTGGAGAGCCTGGACCCCAGGGTCCTCCAGGATCTCGAGGCCCACCAGGCATG AGGGGAGCAAAGGGACGTCGGGGCCCCCGAGGACCGGACGGACCAGCTGGGGAGCAAGGGTCCAGGGGC CTGAAGGGCCCTCCAGGACCCCAGGGCAGACCGGGCCGGCCTGGACAGCAGGGTGTGGCTGGTGAGCGA GGCCACTTGGGCTCGAGAGGCTTTCCTGGCATCCCGGGTCCCTCAGGCCCCCCAGGCACCAAGGGCCTC CCAGGAGAACCGGGCCCTCAGGGACCCCAGGGGCCAATTGGGCCTCCAGGAGAGATGGGACCCAAGGGG CCGCCTGGTGCAGTGGGAGAACCGGGCCTTCCTGGGGAAGCCGGGATGAAGGGTGACCTTGGACCCCTG GGCACTCCTGGGGAGCAGGGCCTCATTGGGCAACGGGGAGAGCCAGGCCTTGAGGGTGACAGTGGCCCC ATGGGACCTGATGGGCTGAAGGGGGACAGGGGAGACCCAGGGCCTGATGGAGAACATGGCGAGAAAGGC CAGGAAGGGCTGATGGGTGAGGACGGGCCCCCCGGCCCCCCTGGCGTCACTGGTGTCCGGGGTCCTGAA GGAAAATCAGGGAAGCAAGGCGAGAAGGGCCGCACTGGAGCCAAGGGTGCCAAGGGCTATCAAGGACAG CTGGGTGAGATGGGCGTCCCTGGAGACCCTGGACCCCCTGGCACTCCAGGCCCTAAAGGGTCCCGGGGC AGCCTGGGACCAACGGGTGCTCCGGGACGCATGGGGGCCCAAGGAGAACCGGGACTGGCTGGTTATGAT GGACACAAAGGCATTGTGGGACCCCTTGGACCTCCTGGACCAAAAGGCGAAAAGGGGGAGCAGGGCGAG GACGGCAAGGCTGAGGGGCCCCCTGGGCCACCTGGAGATCGGGGCCCTGTGGGTGATCGAGGAGACCGC GGGGAACCGGGAGACCCTGGGTACCCTGGACAGGAGGGTGTGCAAGGCCTCCGTGGAAAGCCAGGCCAG CAGGGCCAACCCGGGCATCCGGGACCCCGGGGGTGGCCGGGACCCAAAGGATCGAAAGGCGCAGAGGGA CCAAAGGGAAAGCAAGGCAAGGCAGGGGCCCCAGGCCGGAGGGGGGTCCAGGGCCTGCAGGGGCTGCCA GGGCCCCGGGGCGTGGTGGGGAGACAGGGCCTCGAGGGCATCGCTGGACCAGATGGGCTTCCTGGCAGG GACGGGCAAGCAGGACAGCAGGGGGAGCAGGGAGACGATGGGGACCCTGGCCCCATGGGCCCTGCTGGG AAGAGAGGAAATCCAGGTGTGGCCGGCTTACCTGGAGCACAGGGACCCCCAGGATTCAAGGGTGAGAGT GGGTTACCCGGACAGCTGGGTCCCCCTGGCAAGCGAGGAACAGAGGGCAGAACGGGGCTCCCTGGAAAC CAGGGGGAGCCTGGGTCCAAAGGCCAGCCGGGCGACTCTGGCGAGATGGGCTTCCCAGGAATGGCAGGT CTCTTCGGACCCAAGGGCCCGCCTGGAGACATTGGCTTCAAAGGCATCCAGGGCCCTCGGGGGCCACCT GGCTTGATGGGAAAGGAAGGCATCGTCGGGCCCCTCGGAATCCTGGGACCTTCGGGACTCCCGGGTCCG AAGGGTGACAAAGGCAGCCGTGGGGACTGGGGATTGCAAGGTCCGAGGGGTCCTCCCGGCCCCAGAGGG CGGCCCGGCCCCCCGGGTCCTCCAGGGGGTCCTATCCAATTGCAACAAGATGATCTTGGGGCAGCTTTC CAGACGTGGATGGACACCAGTGGAGCACTCAGGCCAGAGAGTTACAGCTATCCAGACCGGCTGGTGCTG GACCAGGGAGGAGAGATCTTTAAAACCTTACACTACCTCAGCAACCTCATCCAGAGCATTAAGACGCCC CTGGGCACCAAAGAGAACCCCGCCCGGGTCTGCAGGGACCTCATGGACTGTGAGCAGAAGATGGTGGAT GGTACCTACTGGGTGGATCCAAACCTTGGCTGCTCCTCTGACACCATCGAGGTCTCCTGCAACTTCACT CATGGTGGACAGACGTGTCTCAAGCCCATCACGGCCTCCAAGGTCGAGTTTGCCATCAGCCGGGTCCAG ATGAATTTCCTGCACCTGCTAAGCTCCGAGGTGACCCAGCACATCACCATCCACTGCCTTAACATGACC GTGTGGCAGGAGGGCACTGGGCAGACCCCAGCCAAGCAGGCCGTACGCTTCCGGGCCTGGAATGGACAG ATTTTTGAAGCTGGGGGTCAGTTCCGGCCCGAGGTGTCCATGGATGGCTGCAAGGTCCAAGATGGCCGC TGGCATCAGACACTCTTCACCTTCCGGACCCAAGACCCCCAACAGCTGCCCATCATCAGTGTGGACAAC CTCCCTCCTGCCTCATCAGGGAAGCAGTACCGCCTGGAAGTTGGACCTGCGTGCTTCCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_032888
Insert Size	5583 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_032888.3
RefSeq Size	7818 bp
RefSeq ORF	5583 bp
Locus ID	85301
UniProt ID	Q8IZC6
MW	186.9 kDa
Gene Summary	This gene encodes a member of the fibrillar collagen family, and plays a role during the calcification of cartilage and the transition of cartilage to bone. The encoded protein product is a preproprotein. It includes an N-terminal signal peptide, which is followed by an N-terminal propetide, mature peptide and a C-terminal propeptide. The N-terminal propeptide contains thrombospondin N-terminal-like and laminin G-like domains. The mature peptide is a major triple-helical region. The C-terminal propeptide, also known as COLFI domain, plays crucial roles in tissue growth and repair. Mutations in this gene cause Steel syndrome. Alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been determined. [provided by RefSeq, Sep 2014]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.