Collagen XI alpha 2 (COL11A2) (NM_080680) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DFNA13; DFNB53; FBCG2; HKE5; OSMEDA; OSMEDB; PARP; STL3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC305814 representing NM_080680. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGCGGTGCAGCCGCTGCCATCGCCTCCTCCTCCTCCTACCTCTGGTGCTGGGGCTGAGCGCGGCC CCAGGCTGGGCAGGTGCACCCCCTGTGGATGTGCTCCGGGCCCTGAGGTTCCCCTCCCTCCCTGATGGT GTCCGGAGAGCGAAAGGCATCTGTCCAGCTGATGTGGCCTACCGAGTGGCACGACCTGCCCAGCTCAGT GCACCCACTCGCCAGCTTTTCCCAGGAGGATTTCCCAAAGATTTCTCTCTGCTGACTGTTGTCCGGACC CGCCCTGGTCTCCAAGCTCCCCTCCTGACTCTCTACAGTGCCCAGGGTGTCCGACAGCTGGGCCTGGAG CTGGGCCGACCTGTCCGCTTCCTGTATGAAGACCAGACTGGGCGGCCTCAACCTCCCTCTCAGCCAGTC TTCCGAGGCCTCAGCCTAGCAGATGGCAAGTGGCACCGTGTGGCTGTGGCTGTGAAGGGCCAGTCTGTC ACCCTCATTGTTGACTGCAAGAAGCGAGTCACCCGGCCTCTCCCCCGAAGTGCTCGTCCAGTATTGGAC ACCCATGGAGTGATCATCTTTGGTGCCCGTATTCTGGATGAAGAAGTCTTTGAGGGTGATGTCCAGGAG CTGGCCATTGTCCCAGGGGTCCAGGCAGCCTATGAATCATGTGAACAGAAGGAGCTGGAATGCGAGGGG GGCCAGAGGGAAAGACCCCAAAACCAACAGCCTCACAGAGCCCAGAGATCTCCACAGCAGCAACCATCA AGACTTCACAGGCCACAAAATCAGGAACCCCAGAGCCAGCCCACTGAGTCTCTCTACTATGACTACGAG CCCCCCTATTATGATGTGATGACTACGGGGACAACCCCTGATTATCAGGACCCCACCCCAGGTGAAGAG GAAGAAATCCTGGAGTCGAGCCTCTTGCCACCCCTTGAGGAGGAGCAGACAGATCTCCAGGTCCCCCCC ACAGCCGACAGGTTCCAGGCAGAGGAATATGGGGAGGGTGGCACAGACCCCCCTGAAGGGCCCTACGAT TACACCTATGGCTATGGGGATGATTATCGTGAGGAGACAGAGCTTGGCCCTGCCCTCTCTGCGGAGACA GCCCACTCAGGAGCCGCTGCCCATGGACCCCGAGGGCTGAAGGGAGAGAAAGGAGAGCCTGCAGTGTTG GAACCTGGTATGCTCGTGGAGGGGCCCCCTGGCCCAGAAGGCCCTGCGGGATTGATTGGTCCCCCTGGC ATCCAGGGGAACCCAGGCCCAGTTGGAGACCCTGGAGAGAGGGGCCCCCCTGGCCGAGCAGGGCTCCCT GGATCAGATGGGGCTCCTGGTCCTCCTGGCACATCTCTCATGCTCCCATTCCGGTTTGGCAGTGGTGGG GGTGACAAGGGCCCTGTGGTGGCGGCCCAGGAGGCTCAGGCCCAGGCGATCCTGCAGCAGGCGAGGCTG GCGCTCCGTGGACCCCCTGGCCCCATGGGATACACAGGGCGCCCTGGACCCTTGGGCCAACCTGGGAGC CCTGGCCTGAAAGGAGAGTCTGGAGACTTAGGACCTCAGGGCCCCAGAGGACCTCAGGGCCTCACAGGC CCTCCTGGCAAGGCTGGGCGAAGGGGCCGGGCAGGTGCTGATGGAGCCCGAGGGATGCCTGGAGATCCT GGAGTGAAGGGTGACCGAGGTTTTGATGGACTCCCAGGGCTCCCTGGAGAGAAGGGCCATAGGGGTGAT ACTGGTGCCCAGGGCCTTCCTGGTCCCCCTGGTGAGGATGGAGAGAGGGGAGATGACGGGGAGATTGGG CCTCGAGGGCTGCCTGGAGAGTCGGGACCTCGAGGTCTCCTTGGCCCCAAAGGCCCACCTGGTATTCCT GGACCCCCTGGCGTCCGAGGCATGGATGGTCCCCAGGGCCCCAAAGGGAGCTTGGGACCCCAGGGAGAG CCAGGACCTCCTGGACAACAGGGCACCCCTGGGACCCAGGGTCTTCCCGGGCCCCAGGGTGCCATCGGC CCTCATGGAGAGAAGGGTCCTCAAGGGAAGCCAGGGCTCCCCGGCATGCCTGGCTCAGACGGACCCCCG GGTCACCCAGGGAAGGAAGGTCCCCCTGGAACCAAAGGAAACCAGGGTCCCTCTGGACCTCAGGGACCT CTAGGATACCCAGGACCTCGAGGGGTCAAGGGTGTGGACGGAATTCGGGGTCTGAAGGGTCATAAGGGT GAGAAGGGTGAGGATGGCTTTCCTGGGTTCAAAGGTGACATAGGCGTGAAAGGTGACAGGGGCGAAGTT GGAGTCCCTGGTTCCAGGGGAGAGGATGGTCCTGAGGGGCCAAAGGGACGCACTGGACCGACTGGAGAC CCTGGGCCCCCAGGGCTCATGGGCGAGAAGGGCAAGCTGGGTGTTCCTGGTCTGCCTGGCTATCCTGGA CGTCAGGGACCCAAGGGGTCCCTAGGATTTCCTGGCTTTCCTGGTGCCAGTGGAGAGAAGGGAGCCCGG GGCCTGTCGGGGAAGTCAGGGCCTCGGGGAGAACGGGGCCCCACGGGTCCACGGGGTCAGCGGGGACCC CGAGGTGCCACTGGGAAGTCTGGAGCTAAGGGAACATCTGGTGGTGATGGCCCCCATGGGCCCCCTGGA GAGAGGGGCCTCCCTGGACCTCAGGGTCCCAACGGGTTTCCTGGACCGAAAGGACCCCCGGGCCCCCCT GGGAAGGATGGGCTGCCGGGACACCCAGGCCAAAGAGGAGAAGTGGGTTTCCAAGGGAAGACCGGCCCC CCTGGTCCTCCAGGAGTGGTGGGACCTCAGGGAGCAGCAGGAGAAACCGGCCCTATGGGGGAGAGAGGT CACCCAGGCCCCCCGGGGCCCCCTGGAGAGCAGGGACTACCTGGGACAGCTGGAAAAGAAGGAACAAAG GGTGACCCTGGTCCCCCTGGGGCCCCAGGGAAGGATGGTCCTGCTGGTCTGAGGGGATTCCCAGGAGAG AGAGGCCTCCCAGGCACTGCTGGTGGACCTGGTTTGAAGGGGAATGAAGGTCCGTCTGGCCCCCCTGGC CCTGCAGGCTCCCCTGGGGAACGAGGTGCAGCAGGATCAGGGGGACCCATTGGTCCGCCAGGGCGCCCA GGCCCGCAGGGTCCCCCTGGAGCAGCAGGAGAGAAAGGTGTCCCAGGTGAGAAGGGCCCCATTGGCCCG ACTGGCCGAGATGGAGTGCAGGGTCCTGTGGGGCTTCCTGGTCCTGCTGGGCCTCCAGGTGTGGCTGGA GAGGATGGAGACAAGGGTGAGGTGGGGGACCCCGGACAGAAGGGCACCAAAGGGAACAAGGGTGAACAT GGCCCTCCTGGACCCCCTGGACCCATTGGTCCTGTGGGGCAGCCTGGAGCAGCGGGAGCAGATGGGGAG CCCGGAGCTCGGGGACCCCAGGGACACTTTGGAGCCAAAGGTGATGAAGGAACAAGAGGATTCAATGGG CCCCCAGGACCCATTGGCCTACAGGGTTTGCCAGGCCCCTCTGGGGAGAAGGGAGAAACAGGAGATGTG GGTCCTATGGGACCACCTGGCCCCCCAGGACCTCGAGGTCCAGCTGGACCCAATGGCGCTGATGGCCCA CAAGGTCCCCCAGGAGGTGTTGGGAACCTGGGTCCCCCTGGAGAGAAGGGGGAACCAGGAGAGTCAGGA TCTCCAGGGATCCAGGGCGAGCCAGGTGTCAAGGGTCCACGCGGGGAACGTGGAGAGAAAGGAGAGTCG GGGCAGCCAGGAGAGCCAGGGCCACCAGGGCCTAAAGGCCCCACAGGCGATGATGGCCCCAAAGGGAAC CCTGGTCCTGTTGGTTTTCCTGGTGACCCTGGCCCCCCTGGAGAAGGTGGCCCTCGGGGCCAGGATGGT GCTAAGGGTGACCGAGGCGAGGATGGTGAGCCAGGACAGCCTGGATCCCCTGGTCCCACCGGGGAGAAT GGACCCCCAGGGCCACTTGGAAAGCGAGGTCCTGCTGGCTCGCCTGGTTCCGAGGGGCGACAAGGAGGG AAGGGAGCCAAGGGAGATCCTGGCGCTATAGGTGCCCCGGGGAAGACAGGCCCGGTGGGTCCTGCAGGC CCAGCAGGGAAACCTGGCCCTGATGGTCTGAGGGGGCTCCCAGGCTCAGTGGGTCAGCAAGGCCGACCT GGAGCTACAGGCCAGGCTGGGCCCCCAGGTCCTGTGGGACCCCCAGGGCTGCCTGGTCTCCGGGGCGAT GCTGGAGCCAAGGGAGAGAAGGGCCACCCAGGTCTCATTGGACTGATTGGGCCCCCGGGTGAGCAGGGA GAGAAGGGAGATCGGGGACTTCCTGGGCCTCAGGGCTCCCCTGGGCAGAAGGGTGAGATGGGTATCCCA GGAGCATCCGGCCCCATTGGTCCTGGAGGTCCCCCCGGCCTCCCCGGACCTGCTGGCCCCAAAGGAGCC AAAGGAGCCACAGGCCCAGGCGGACCCAAGGGAGAGAAGGGTGTGCAGGGCCCTCCAGGACACCCGGGT CCCCCAGGCGAGGTGATCCAGCCACTGCCCATTCAGATGCCCAAGAAGACTCGGCGCTCGGTGGATGGA AGCCGTCTGATGCAGGAAGATGAGGCCATACCGACCGGGGGAGCCCCCGGCAGTCCTGGGGGGCTGGAG GAGATCTTTGGCTCACTCGACTCCCTGCGGGAGGAGATCGAGCAGATGAGGCGGCCAACAGGGACCCAG GACAGCCCTGCTCGCACCTGCCAGGACCTGAAGCTGTGCCACCCAGAGCTTCCCGATGGAGAGTACTGG GTCGACCCCAACCAGGGCTGTGCTCGGGATGCCTTCCGAGTTTTCTGCAACTTCACAGCAGGGGGTGAG ACCTGTGTGACGCCTAGGGATGACGTCACGCAGTTCTCTTACGTGGACTCAGAGGGCTCCCCAGTGGGT GTGGTCCAGCTCACCTTCCTGCGGCTGCTCAGCGTCTCAGCCCACCAGGACGTCTCCTACCCCTGCTCT GGAGCAGCCCGTGACGGTCCCCTGAGACTCCGTGGGGCCAATGAGGATGAGCTGAGCCCGGAGACTAGC CCCTATGTCAAAGAATTCAGAGATGGCTGCCAGACACAGCAAGGCCGGACGGTGCTGGAGGTGCGAACG CCTGTGCTGGAGCAGCTGCCAGTGCTGGATGCCTCCTTCTCAGACCTGGGAGCCCCACCGAGGCGGGGA GGGGTGCTGCTGGGGCCTGTCTGCTTCATGGGATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_080680
Insert Size	5211 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_080680.2
RefSeq Size	6425 bp
RefSeq ORF	5211 bp
Locus ID	1302
UniProt ID	P13942
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	ECM-receptor interaction, Focal adhesion
MW	171.8 kDa
Gene Summary	This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. It is located on chromosome 6 very close to but separate from the gene for retinoid X receptor beta. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Proteolytic processing of this type XI chain produces PARP, a proline/arginine-rich protein that is an amino terminal domain. Mutations in this gene are associated with type III Stickler syndrome, otospondylomegaepiphyseal dysplasia (OSMED syndrome), Weissenbacher-Zweymuller syndrome, autosomal dominant non-syndromic sensorineural type 13 deafness (DFNA13), and autosomal recessive non-syndromic sensorineural type 53 deafness (DFNB53). Alternative splicing results in multiple transcript variants. A related pseudogene is located nearby on chromosome 6. [provided by RefSeq, Jul 2009] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). There are no publicly available human transcripts representing the full-length exon combination of this variant, but the region of variation is supported by experimental data in PMIDs 8663204 and 8838804, by homology data in mouse, and the full-length sequence is represented in accession U32169.1:AAC50214.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.

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