TSPEAR (NM_144991) Human Untagged Clone
CAT＃: SC306203
TSPEAR (untagged)-Human thrombospondin-type laminin G domain and EAR repeats (TSPEAR)
CNY 13020.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | C21orf29; DFNB98; ECTD14; TSP-EAR |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC306203 representing NM_144991.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTCTGCCCTGCTGAGTCTGTGTTTTGTGCTGCCCCTGGCGGCCCCCGGCCACGGCACGCAGGGTTGG GAGCCCTGCACAGACCTGCGCCCCCTGGACATCCTGGCGGAAGTGGTCCCTTCTGATGGCGCCACAAGC GGGATCAGGATAGTTCAGGTTCACGGTGCACGGGGACTCCAGCTCTCAGTAGCCGCCCCCCGCACCATG AGCTTCCCAGCATCCAGGATTTTCTCCCAGTGTGACCTCTTCCCTGAAGAATTTTCCATCGTCGTAACT TTGAGAGTTCCCAATCTTCCACCCAAGAGGAACGAGTACCTGCTGACGGTGGTGGCAGAGGAGAGCGAC CTGCTGCTGCTCGGCCTGCGGTTGTCACCTGCCCAGCTGCACTTCCTGTTCCTTCGCGAGGACACGGCC GGCGCCTGGCAGACCCGAGTGTCCTTCCGCAGCCCGGCCCTGGTGGATGGCCGCTGGCACACACTGGTC CTGGCTGTGTCCGCAGGCGTCTTCTCCCTCACCACGGACTGCGGCCTCCCGGTGGACATAATGGCCGAT GTGCCCTTCCCAGCCACCCTGTCAGTGAAAGGAGCTCGATTCTTCGTCGGCAGCCGGAGGAGAGCCAAA GGCCTGTTCATGGGACTGGTGAGGCAACTGGTCCTGCTGCCGGGCTCAGACGCCACCCCAAGGCTGTGT CCCAGCAGGAACGCCCCGCTGGCGGTGCTGTCCATCCCACGGGTCCTGCAGGCTCTCACGGGGAAGCCA GAAGATAACGAGGTGCTAAAATATCCCTATGAAACCAACATTCGAGTGACGCTGGGACCCCAGCCACCG TGTACCGAGGTGGAAGACGCCCAGTTCTGGTTTGATGCCAGCCGGAAGGGCCTGTATCTGTGTGTTGGC AACGAGTGGGTCTCCGTGTTAGCAGCCAAAGAAAGACTGGACTACGTGGAGGAGCATCAGAACTTGTCC ACCAACTCAGAGACCCTGGGCATTGAGGTGTTCCGCATCCCTCAGGTGGGGCTCTTTGTGGCCACAGCC AATCGCAAAGCCACATCCGCCGTCTACAAGTGGACCGAAGAGAAGTTCGTCTCATATCAGAACATCCCC ACGCACCAAGCACAGGCCTGGAGGCATTTCACCATCGGGAAAAAGATCTTCCTGGCAGTGGCTAATTTT GAACCAGATGAGAAGGGTCAGGAGTTCTCTGTCATTTACAAATGGAGCCACAGAAAGCTGAAGTTTACC CCATATCAGAGCATTGCCACACACAGCGCCCGAGACTGGGAGGCCTTCGAGGTGGATGGGGAGCACTTC CTGGCGGTGGCCAACCACCGGGAAGGCGACAACCACAACATCGACAGTGTCATCTACAAGTGGAACCCG GCAACCCGGCTCTTCGAGGCCAACCAGACCATCGCCACCTCCGGCGCCTACGACTGGGAGTTCTTCAGT GTGGGGCCCTACTCGTTCCTGGTGGTGGCCAACACCTTCAACGGCACCTCCACCAAGGTGCACTCGCAC CTCTACATCCGACTCCTGGGCTCCTTCCAGCTCTTCCAGTCCTTCCCGACGTTCGGTGCTGCAGACTGG GAGGTCTTCCAGATCGGGGAGAGGATCTTCCTCGCTGTGGCAAACAGTCACAGCTACGATGTGGAGATG CAAGTCCAGAATGATTCCTATGTCATCAACTCCGTCATCTACGAGCTGAACGTGACCGCGCAGGCCTTT GTCAAGTTCCAGGACATTCTCACCTGCAGTGCTCTGGACTGGGAGTTTTTCTCGGTGGGAGAAGATTAT TTCCTGGTGGTGGCCAACTCCTTCGATGGGCGTACCTTCTCGGTGAACAGTATTATTTACAGGTGGCAG GGCTACGAGGGCTTCGTGGCGGTGCACAGCCTCCCCACCGTCGGCTGCAGGGACTGGGAGGCCTTCAGC ACCACGGCTGGTGCCTACCTCATCTACTCCAGCGCCAAGGAGCCCCTCTCCAGGGTCCTGCGGCTGAGG ACACGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_144991 |
| Insert Size | 2010 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_144991.2 |
| RefSeq Size | 3980 bp |
| RefSeq ORF | 2010 bp |
| Locus ID | 54084 |
| UniProt ID | Q8WU66 |
| MW | 74.9 kDa |
| Gene Summary | This gene encodes a protein that contains a N-terminal thrombospondin-type laminin G domain and several tandem arranged epilepsy-associated repeats (EARs). A mutation in this gene is the cause of autosomal recessive deafness-98. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012] Transcript Variant: This variant (1) encodes the longer isoform (1). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216516 | TSPEAR (Myc-DDK-tagged)-Human thrombospondin-type laminin G domain and EAR repeats (TSPEAR) |
CNY 4904.00 |
|
| RC216516L3 | Lenti ORF clone of Human thrombospondin-type laminin G domain and EAR repeats (TSPEAR), Myc-DDK-tagged |
CNY 9030.00 |
|
| RC216516L4 | Lenti ORF clone of Human thrombospondin-type laminin G domain and EAR repeats (TSPEAR), mGFP tagged |
CNY 9030.00 |
|
| RG216516 | TSPEAR (tGFP-tagged) - Human thrombospondin-type laminin G domain and EAR repeats (TSPEAR) |
CNY 7890.00 |
