NUF2 (NM_145697) Human Untagged Clone
CAT＃: SC306261
NUF2 (untagged)-Human NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae) (NUF2), transcript variant 1
CNY 5488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CDCA1; CT106; NUF2R |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene sequence for NM_145697 edited
GATTTTTGACTTTGCTTGTAGCTGCTCCCCGAACTCGCCGTCTTCCTGTCGGCGGCCGGC ACTGTAGATTAACAGGAAACTTCCAAGATGGAAACTTTGTCTTTCCCCAGATATAATGTA GCTGAGATTGTGATTCATATTCGCAATAAGATCTTAACAGGAGCTGATGGTAAAAACCTC ACCAAGAATGATCTTTATCCAAATCCAAAGCCTGAAGTCTTGCACATGATCTACATGAGA GCCTTACAAATAGTATATGGAATTCGACTGGAACATTTTTACATGATGCCAGTGAACTCT GAAGTCATGTATCCACATTTAATGGAAGGCTTCTTACCATTCAGCAATTTAGTTACTCAT CTGGACTCATTTTTGCCTATCTGCCGGGTGAATGACTTTGAGACTGCTGATATTCTATGT CCAAAAGCAAAACGGACAAGTCGGTTTTTAAGTGGCATTATCAACTTTATTCACTTCAGA GAAGCATGCCGTGAAACGTATATGGAATTTCTTTGGCAATATAAATCCTCTGCGGACAAA ATGCAACAGTTAAACGCCGCACACCAGGAGGCATTAATGAAACTGGAGAGACTTGATTCT GTTCCAGTTGAAGAGCAAGAAGAGTTCAAGCAGCTTTCAGATGGAATTCAGGAGCTACAA CAATCACTAAATCAGGATTTTCATCAAAAAACGATAGTGCTGCAAGAGGGAAATTCCCAA AAGAAGTCAAATATTTCAGAGAAAACCAAGCGTTTGAATGAACTAAAATTGTCGGTGGTT TCTTTGAAAGAAATACAAGAGAGTTTGAAAACAAAAATTGTGGATTCTCCAGAGAAGTTA AAGAATTATAAAGAAAAAATGAAAGATACGGTCCAGAAGCTTAAAAATGCCAGACAAGAA GTGGTGGAGAAATATGAAATCTATGGAGACTCAGTTGACTGCCTGCCTTCATGTCAGTTG GAAGTGCAGTTATATCAAAAGAAAATACAGGACCTTTCAGATAATAGGGAAAAATTAGCC AGTATCTTAAAGGAGAGCCTGAACTTGGAGGACCAAATTGAGAGTGATGAGTCAGAACTG AAGAAATTGAAGACTGAAGAAAATTCGTTCAAAAGACTGATGATTGTGAAGAAGGAAAAA CTTGCCACAGCACAATTCAAAATAAATAAGAAGCATGAAGATGTTAAGCAATACAAACGC ACAGTAATTGAGGATTGCAATAAAGTTCAAGAAAAAAGAGGTGCTGTCTATGAACGAGTA ACCACAATTAATCAAGAAATCCAAAAAATTAAACTTGGAATTCAACAACTAAAAGATGCT GCTGAAAGGGAGAAACTGAAGTCCCAGGAAATATTTCTAAACTTGAAAACTGCTTTGGAG AAATACCACGACGGTATTGAAAAGGCAGCAGAGGACTCCTATGCTAAGATAGATGAGAAG ACAGCTGAACTGAAGAGGAAGATGTTCAAAATGTCAACCTGATTAACAAAATTACATGTC TTTTTGTAAATGGCTTGCCATCTTTTAATTTTCTATTTAGAAAGAAAAGTTGAAGCGAAT GGAAGTATCAGAAGTACCAAATAATGTTGGCTTCATCAGTTTTTATACACTCTCATAAGT AGTTAATAAGATGAATTTAATGTAGGCTTTTATTAATTTATAATTAAAATAACTTGTGCA GCTATTCATGTCTCTACTCTGCCCCTTGTTGTAAATAGTTTGAGTAAAACAAAACTAGTT ACCTTTGAAATATATATATTTTTTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_145697 |
| Insert Size | 1800 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | Th eORF of this clone has been fully sequenced and found to be a perfect match to NM_145697.1. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_145697.1, NP_663735.1 |
| RefSeq Size | 2003 bp |
| RefSeq ORF | 1395 bp |
| Locus ID | 83540 |
| UniProt ID | Q9BZD4 |
| Protein Families | Druggable Genome |
| Gene Summary | This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218603 | NUF2 (Myc-DDK-tagged)-Human NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae) (NUF2), transcript variant 1 |
CNY 5488.00 |
|
| RC218603L1 | Lenti ORF clone of Human NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae) (NUF2), transcript variant 1, Myc-DDK-tagged |
CNY 7888.00 |
|
| RC218603L2 | Lenti ORF clone of Human NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae) (NUF2), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RC218603L3 | Lenti ORF clone of Human NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae) (NUF2), transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC218603L4 | Lenti ORF clone of Human NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae) (NUF2), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RG218603 | NUF2 (tGFP-tagged) - Human NUF2, NDC80 kinetochore complex component, homolog (S. cerevisiae) (NUF2), transcript variant 1 |
CNY 7088.00 |
