GEFT (ARHGEF25) (NM_182947) Human Untagged Clone
CAT＃: SC307541
ARHGEF25 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 25 (ARHGEF25), transcript variant 1
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GEFT; p63RhoGEF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC307541 representing NM_182947.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCGGGGGGGGCACAAAGGGGGTCGCTGTGCCTGTCCCCGTGTGATCCGAAAAGTGCTGGCAAAATGC GGCTGCTGCTTCGCCCGGGGGGGACGTGAATCCTATTCCATTGCGGGCAGTGAGGGGAGTATATCGGCT TCTGCTGCCTCCGGTCTGGCTGCCCCCTCTGGCCCCAGCTCTGGCCTCAGCTCTGGCCCCTGTTCCCCA GGCCCCCCAGGGCCCGTCAGTGGCCTGAGGAGATGGTTGGATCATTCCAAACATTGTCTCAGTGTGGAA ACTGAGGCAGACAGTGGTCAGGCAGGACCATATGAGAACTGGATGTTGGAGCCAGCTCTAGCCACAGGA GAGGAGCTGCCGGAACTGACCTTGCTGACCACACTGTTGGAGGGCCCTGGAGATAAGACGCAGCCACCT GAAGAGGAGACTTTGTCCCAAGCCCCTGAGAGTGAGGAGGAACAGAAGAAGAAGGCTCTGGAAAGGAGT ATGTATGTCCTGAGTGAACTGGTAGAAACAGAGAAAATGTACGTGGACGACTTGGGGCAGATTGTGGAG GGTTATATGGCCACCATGGCTGCTCAGGGGGTCCCCGAGAGTCTTCGAGGCCGTGACAGGATTGTGTTT GGGAATATCCAGCAAATCTATGAGTGGCACCGAGACTATTTCTTGCAAGAGCTACAACGGTGTCTGAAA GATCCTGATTGGCTGGCTCAGCTATTCATCAAACACGAGCGCCGGCTGCATATGTATGTGGTGTACTGT CAGAATAAGCCCAAGTCAGAGCATGTGGTGTCAGAGTTTGGGGACAGCTACTTTGAGGAGCTCCGGCAG CAGCTGGGGCACCGCCTGCAGCTGAACGACCTCCTCATCAAACCTGTGCAGCGGATCATGAAATACCAG CTGCTGCTCAAGGATTTTCTCAAGTATTACAATAGAGCTGGGATGGATACTGCAGACCTAGAGCAAGCT GTGGAGGTCATGTGCTTTGTGCCCAAGCGCTGCAACGATATGATGACGCTGGGGAGATTGCGGGGATTT GAGGGCAAACTGACTGCTCAGGGGAAGCTCTTGGGCCAGGACACTTTCTGGGTCACCGAGCCTGAGGCT GGAGGGCTGCTGTCTTCCCGAGGTCGAGAGAGGCGCGTCTTCCTCTTTGAGCAAATCATCATCTTCAGT GAAGCCCTGGGAGGAGGAGTGAGAGGTGGAACACAGCCTGGATATGTATACAAGAACAGCATTAAGGTG AGCTGCCTGGGACTGGAGGGGAACCTCCAAGGTGACCCTTGCCGCTTTGCACTGACCTCCAGAGGGCCA GAGGGTGGGATCCAGCGCTATGTCCTGCAGGCTGCAGACCCTGCTATCAGTCAGGCCTGGATCAAGCAT GTGGCTCAGATCTTGGAGAGCCAACGGGACTTCCTCAACGCATTGCAGTCACCCATTGAGTACCAGAGA CGGGAGAGCCAGACCAACAGCCTGGGGCGGCCAAGAGGGCCTGGAGTGGGGAGCCCTGGAAGAATTCGG CTTGGAGATCAGGCCCAGGGCAGCACACACACACCCATCAATGGCTCTCTCCCCTCTCTGCTGCTGTCA CCCAAAGGGGAGGTGGCCAGAGCCCTCTTGCCACTGGATAAACAGGCCCTTGGTGACATCCCCCAGGCT CCCCATGACTCTCCTCCAGTCTCTCCAACTCCAAAAACCCCTCCCTGCCAAGCCAGACTTGCCAAGCTG GATGAAGATGAGCTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_182947 |
| Insert Size | 1743 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_182947.3 |
| RefSeq Size | 2563 bp |
| RefSeq ORF | 1743 bp |
| Locus ID | 115557 |
| UniProt ID | Q86VW2 |
| MW | 63.9 kDa |
| Gene Summary | Rho GTPases alternate between an inactive GDP-bound state and an active GTP-bound state, and GEFs facilitate GDP/GTP exchange. This gene encodes a guanine nucleotide exchange factor (GEF) which interacts with Rho GTPases involved in contraction of vascular smooth muscles, regulation of responses to angiotensin II and lens cell differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (1) represents the longest transcript (1) and encodes isoform 1. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207415 | ARHGEF25 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 25 (ARHGEF25), transcript variant 1 |
CNY 4320.00 |
|
| RC207415L1 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 25 (ARHGEF25), transcript variant 1, Myc-DDK-tagged |
CNY 6720.00 |
|
| RC207415L2 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 25 (ARHGEF25), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RC207415L3 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 25 (ARHGEF25), transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC207415L4 | Lenti ORF clone of Human Rho guanine nucleotide exchange factor (GEF) 25 (ARHGEF25), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RG207415 | ARHGEF25 (tGFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 25 (ARHGEF25), transcript variant 1 |
CNY 5920.00 |
