KIF1A (NM_004321) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ATSV; C2orf20; HSN2C; MRD9; NESCAVS; SPG30; UNC104
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC308993 representing NM_004321. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCGGGGCTTCGGTGAAGGTGGCGGTGCGGGTCCGCCCCTTCAATTCCCGGGAAATGAGCCGTGAC TCCAAGTGCATCATTCAGATGTCTGGAAGCACCACCACCATTGTTAACCCCAAACAGCCCAAGGAGACG CCCAAAAGCTTCAGCTTTGACTACTCCTACTGGTCGCACACCTCACCTGAGGACATCAACTACGCGTCG CAGAAGCAGGTGTACCGGGACATCGGCGAGGAGATGCTGCAGCATGCCTTTGAGGGATACAACGTGTGC ATCTTCGCCTATGGGCAGACGGGTGCCGGCAAGTCCTACACCATGATGGGCAAGCAGGAGAAGGACCAG CAGGGCATCATCCCACAGCTCTGCGAGGACCTCTTCTCTCGGATCAACGACACGACCAACGACAACATG TCCTACTCCGTGGAGGTCAGCTACATGGAGATTTACTGTGAGCGCGTCCGTGACCTCCTGAACCCCAAG AACAAGGGCAACCTTCGCGTGAGGGAGCACCCACTGCTGGGGCCCTACGTGGAGGACCTCTCCAAGCTG GCTGTCACCTCCTACAATGACATCCAGGACCTCATGGACTCAGGGAACAAGGCCAGGACCGTGGCGGCC ACCAACATGAATGAGACCAGCAGTCGCTCCCACGCCGTCTTCAACATCATCTTCACCCAGAAGCGCCAT GACGCAGAGACCAATATCACCACGGAGAAGGTGAGCAAAATCAGCCTGGTGGACCTGGCTGGGAGCGAG CGGGCTGACTCCACGGGAGCCAAGGGCACGCGCCTCAAGGAGGGGGCCAACATCAACAAGTCGCTGACC ACCCTGGGCAAGGTCATCTCCGCCCTGGCTGAAATGGACTCCGGACCCAACAAGAACAAGAAAAAGAAG AAGACAGATTTCATTCCGTACCGAGATTCCGTGTTGACCTGGCTCCTCCGGGAAAACCTGGGCGGTAAC TCAAGGACAGCTATGGTGGCAGCCTTGAGTCCTGCAGACATCAACTACGATGAGACCCTTAGCACGCTG AGGTATGCTGACCGGGCCAAGCAGATCCGCTGCAATGCTGTCATCAATGAGGACCCCAACAACAAGCTG ATCCGCGAGCTGAAGGATGAGGTGACCCGGCTGCGGGACCTTCTGTACGCCCAGGGTCTTGGCGACATC ACTGACATGACCAATGCCCTGGTGGGTATGAGCCCCTCATCCTCGCTCTCAGCCCTGTCCAGCCGCGCG GCCTCCGTGTCCAGCCTCCACGAGCGCATCTTGTTTGCCCCGGGCAGCGAGGAGGCCATTGAAAGACTG AAGGAAACAGAGAAGATCATAGCTGAGCTCAATGAGACCTGGGAGGAGAAGCTGCGGCGGACAGAAGCC ATCCGGATGGAGAGGGAAGCCCTGCTGGCCGAGATGGGTGTGGCCATGAGGGAGGATGGCGGCACCTTG GGCGTATTCTCTCCCAAAAAGACACCACACCTCGTCAACCTGAACGAGGACCCGCTGATGTCTGAGTGC CTGCTCTACTACATCAAGGATGGGATCACCAGAGTGGGCAGGGAGGATGGCGAGAGGCGGCAGGACATT GTTCTGAGTGGGCACTTCATCAAGGAGGAGCACTGCGTCTTCCGGAGCGACTCCAGGGGAGGCAGCGAA GCTGTGGTGACCTTGGAGCCCTGTGAGGGGGCAGACACCTACGTCAATGGCAAGAAAGTCACAGAGCCC AGCATCCTGCGTTCAGGAAACCGCATCATCATGGGTAAGAGCCATGTGTTCCGGTTCAACCACCCCGAG CAGGCCCGGCAGGAGCGTGAGCGCACGCCTTGTGCGGAGACGCCAGCTGAGCCTGTGGACTGGGCCTTC GCCCAGCGTGAGCTGCTGGAGAAGCAGGGCATCGACATGAAGCAGGAGATGGAGCAGAGGCTCCAGGAA CTGGAGGACCAGTACCGCCGCGAGCGGGAGGAGGCCACCTACCTGCTGGAGCAGCAGCGGCTGGACTAT GAGAGCAAGCTGGAGGCTCTGCAGAAGCAGATGGACTCCAGGTACTACCCGGAGGTGAACGAGGAGGAG GAGGAGCCCGAGGATGAAGTCCAGTGGACAGAGCGGGAGTGTGAGCTGGCGCTCTGGGCCTTCCGGAAG TGGAAGTGGTACCAGTTCACGTCTCTGCGGGACCTGCTGTGGGGCAACGCCATCTTCCTCAAGGAGGCC AATGCCATCAGCGTGGAGCTGAAAAAGAAGGTACAATTCCAGTTTGTCCTCCTGACGGACACACTCTAC TCCCCTCTGCCACCCGACCTGCTGCCCCCAGAGGCCGCCAAAGACCGAGAGACGCGGCCCTTCCCCCGC ACCATTGTGGCCGTGGAGGTCCAGGACCAGAAGAACGGGGCCACCCACTACTGGACGCTGGAGAAGCTC AGGCAGCGTCTGGACCTGATGCGGGAGATGTACGACCGCGCTGCAGAGGTGCCCTCCAGTGTCATCGAG GACTGTGACAACGTGGTGACCGGCGGAGACCCCTTCTATGACCGCTTCCCCTGGTTCCGGCTGGTGGGC AGGGCCTTCGTGTACCTGAGCAACCTGCTGTACCCCGTTCCCCTGGTACACCGTGTGGCAATCGTCAGC GAGAAGGGCGAGGTGAAGGGCTTCCTCCGCGTGGCCGTCCAGGCCATCTCAGCCGATGAAGAGGCCCCT GATTATGGCTCTGGCGTCCGCCAGTCGGGAACTGCTAAAATCTCCTTTGATGACCAGCATTTTGAAAAG TTCCAGTCCGAGTCTTGCCCCGTGGTGGGGATGTCCCGCTCGGGAACCTCCCAGGAAGAGCTTCGCATC GTGGAGGGCCAGGGCCAGGGTGCAGACGTGGGGCCCTCAGCCGATGAAGTCAACAACAACACCTGTTCA GCAGTGCCCCCAGAAGGCCTCCTCCTAGACAGCTCTGAGAAAGCCGCCCTGGATGGGCCCCTGGATGCT GCCCTGGACCACCTCCGCCTGGGCAACACCTTCACCTTCCGTGTGACAGTCCTGCAGGCGTCCAGCATC TCTGCCGAATATGCCGACATCTTCTGCCAGTTCAACTTCATCCACCGCCACGACGAGGCCTTCTCCACA GAGCCCCTGAAGAACACAGGCAGAGGCCCCCCACTTGGCTTCTACCACGTCCAGAACATCGCAGTGGAG GTGACCAAGTCCTTCATTGAGTACATCAAGAGCCAGCCCATTGTTTTCGAGGTCTTTGGCCACTACCAG CAGCACCCGTTCCCGCCCCTCTGCAAGGACGTGCTCAGCCCCCTGAGGCCCTCGCGCCGCCACTTCCCT CGGGTCATGCCACTGTCCAAGCCAGTGCCCGCCACCAAGCTCAGCACACTGACGCGGCCCTGTCCGGGA CCCTGCCACTGCAAGTACGACCTGCTGGTCTACTTCGAGATCTGTGAGCTGGAGGCCAACGGCGATTAC ATCCCGGCCGTGGTGGACCACCGTGGGGGCATGCCATGCATGGGGACCTTCCTCCTCCACCAGGGCATC CAGCGACGGATTACGGTGACACTACTGCATGAGACAGGCAGCCATATCCGCTGGAAGGAAGTGCGCGAG CTGGTCGTGGGCCGCATCCGAAACACTCCAGAGACCGACGAGTCCCTGATCGACCCCAACATCTTGTCT CTCAACATCCTCTCTTCCGGATACATCCACCCAGCCCAAGATGACCGGACCTTTTACCAATTTGAGGCT GCGTGGGACAGCTCCATGCACAACTCTCTCCTGCTGAACCGGGTCACCCCTTATCGAGAGAAAATCTAC ATGACACTCTCCGCTTATATCGAGATGGAGAACTGCACCCAGCCGGCTGTTGTCACCAAGGACTTCTGC ATGGTCTTCTATTCCCGTGATGCCAAGCTGCCAGCCTCGCGCTCCATCCGCAACCTCTTTGGCAGTGGG AGCCTTCGGGCCTCAGAGAGTAACCGTGTGACTGGTGTGTACGAGCTCAGCCTGTGCCACGTGGCTGAC GCGGGCAGCCCAGGGATGCAGCGCCGGCGCCGACGAGTCCTGGACACATCTGTGGCCTATGTCCGGGGC GAGGAGAACCTGGCAGGCTGGAGGCCCCGGAGTGACAGTCTCATTCTGGACCACCAGTGGGAGCTGGAG AAGCTGAGCCTCCTGCAGGAGGTGGAGAAGACTAGGCACTACCTGCTCCTGCGGGAGAAGCTGGAGACC GCCCAGCGGCCTGTCCCGGAGGCACTGTCCCCGGCCTTCAGCGAGGACTCTGAGTCCCATGGCTCCTCC AGCGCCTCCTCCCCGCTCTCGGCTGAGGGCCGCCCATCACCCCTGGAGGCTCCCAACGAGAGGCAGCGG GAGCTGGCCGTCAAGTGCTTGCGCCTGCTCACGCACACATTCAACAGAGAGTACACACACAGCCACGTC TGCGTCAGTGCCAGCGAGAGCAAGCTCTCCGAGATGTCTGTCACCCTGCTCCGGGACCCGTCGATGTCC CCTCTAGGGGTGGCCACTCTCACCCCCTCCTCCACTTGCCCCTCTCTGGTTGAAGGGCGGTACGGTGCC ACTGACCTGAGGACCCCGCAGCCCTGCTCCCGGCCAGCCAGCCCAGAGCCCGAGCTGCTGCCAGAGGCC GACTCCAAGAAGCTCCCTTCCCCTGCCCGGGCAACAGAGACAGACAAGGAGCCCCAGCGCCTGCTGGTC CCTGACATCCAGGAGATCCGAGTCAGCCCGATCGTTTCCAAGAAGGGGTACCTGCACTTCCTGGAGCCG CACACGTCAGGCTGGGCCAGGCGCTTCGTGGTGGTGCGGCGCCCCTATGCCTACATGTACAACAGCGAC AAGGACACCGTGGAGCGGTTCGTGCTCAACCTGGCCACTGCCCAGGTGGAGTACAGTGAGGACCAGCAG GCTATGCTCAAGACACCCAACACATTCGCGGTGTGCACGGAACACCGCGGCATCCTGCTGCAGGCCGCC AGCGACAAGGACATGCATGACTGGCTGTACGCCTTCAACCCCCTCCTGGCCGGGACCATACGGTCCAAG CTCTCCAGAAGGAGGTCTGCCCAGATGCGGGTCTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_004321
Insert Size	5073 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_004321.6
RefSeq Size	8743 bp
RefSeq ORF	5073 bp
Locus ID	547
UniProt ID	Q12756
Domains	FHA, kinesin, PH
Protein Families	Druggable Genome
MW	191.1 kDa
Gene Summary	The protein encoded by this gene is a member of the kinesin family and functions as an anterograde motor protein that transports membranous organelles along axonal microtubules. Mutations at this locus have been associated with spastic paraplegia-30 and hereditary sensory neuropathy IIC. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Apr 2012] Transcript Variant: This variant (2) lacks two alternate in-frame exons in the coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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