RGS12 (NM_198229) Human Untagged Clone
CAT＃: SC309374
RGS12 (untagged)-Human regulator of G-protein signaling 12 (RGS12), transcript variant 1
CNY 27930.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC309374 representing NM_198229.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTTAGAGCTGGGGAGGCCTCCAAACGCCCATTGCCTGGGCCGTCGCCCCCAAGGGTGCGGAGTGTG GAGGTTGCCCGGGGGAGGGCCGGCTACGGATTCACGCTTTCGGGACAGGCACCCTGTGTGCTCAGCTGC GTCATGAGAGGGAGCCCTGCGGATTTCGTGGGCCTCCGAGCTGGAGACCAGATACTTGCTGTCAATGAA ATCAACGTGAAAAAAGCATCTCATGAAGATGTAGTGAAATTAATTGGGAAGTGCTCTGGTGTCCTTCAC ATGGTGATTGCTGAAGGCGTCGGCCGCTTCGAATCCTGTTCCAGTGATGAAGAAGGGGGACTCTATGAA GGAAAAGGCTGGCTGAAGCCCAAGCTGGATTCTAAAGCACTAGGTATAAACAGAGCAGAGCGAGTCGTG GAGGAAATGCAGTCTGGTGGAATTTTCAATATGATTTTTGAAAACCCGAGCCTTTGTGCGAGCAATTCA GAGCCCTTGAAATTGAAACAAAGATCCCTTTCAGAGTCGGCCGCAACTCGATTTGATGTTGGACATGAA AGTATAAATAATCCAAATCCCAACATGCTTTCTAAGGAGGAAATATCAAAAGTTATTCATGATGATTCG GTTTTCAGCATTGGACTAGAAAGTCATGACGATTTTGCATTGGATGCAAGTATTTTAAACGTGGCGATG ATCGTGGGCTACTTAGGCTCCATTGAGCTTCCTTCCACGAGCTCCAACCTGGAGTCCGACAGCTTGCAA GCCATCCGCGGCTGCATGCGGCGCCTGCGGGCAGAGCAGAAAATCCACTCGCTGGTGACCATGAAGATC ATGCACGACTGTGTGCAGCTGAGCACTGACAAGGCTGGAGTCGTGGCCGAGTACCCGGCCGAGAAGCTG GCCTTCAGCGCCGTGTGCCCGGACGACCGGCGATTTTTCGGGTTGGTTACCATGCAGACGAATGACGAC GGGAGCCTGGCCCAGGAGGAGGAGGGCGCCCTGCGGACTTCCTGCCACGTGTTCATGGTGGACCCAGAC TTGTTTAATCACAAGATCCACCAAGGCATTGCTCGGCGGTTTGGGTTTGAGTGCACGGCCGACCCAGAC ACCAATGGCTGTCTGGAATTCCCGGCGTCCTCCCTCCCCGTCCTGCAGTTCATCTCTGTCCTGTACCGA GACATGGGTGAGCTGATTGAGGGCATGCGGGCCCGCGCCTTTCTGGACGGGGACGCCGATGCCCACCAG AACAACAGCACCAGCAGCAACAGTGACAGCGGCATTGGGAACTTCCACCAGGAGGAGAAGAGCAACCGG GTCCTTGTGGTGGACCTGGGTGGGAGCTCGAGCAGACACGGCCCCGGAGGCAGCGCGTGGGACGGTGTG GGTGGGAGGGGTGCCCAGCCCTGGGGTGCTCCCTGGACTGGGCCCTTCTGTCCGGACCCCGAAGGGAGC CCCCCATTTGAGGCCGCTCATCAGACTGACAGGTTCTGGGACCTAAACAAGCACCTAGGGCCAGCCTCT CCTGTGGAGGTGCCCCCAGCTTCCTTGAGGAGCTCAGTCCCCCCTTCCAAGAGGGGCACCGTGGGTGCT GGCTGTGGTTTCAACCAGCGCTGGCTCCCGGTCCACGTGCTCCGGGAGTGGCAGTGCGGACACACCAGC GACCAGGACTCTTACACAGATTCCACCGATGGCTGGTCCAGCATCAACTGCGGCACACTGCCCCCTCCT ATGAGCAAGATCCCCGCAGACCGCTACAGGGTGGAGGGCAGCTTCGCGCAGCCCCCGCTGAATGCCCCG AAGAGGGAGTGGTCCAGGAAGGCCTTTGGAATGCAAAGCATTTTTGGTCCCCATCGAAATGTTCGAAAG ACTAAGGAAGATAAAAAGGGCTCAAAATTTGGGCGGGGAACTGGACTCACTCAGCCTTCTCAACGCACG TCTGCTCGGAGATCATTTGGGAGATCCAAGAGATTCAGTATCACTCGCTCCCTTGATGATCTTGAGTCT GCAACTGTGTCTGATGGCGAGTTGACGGGCGCCGACCTGAAGGACTGCGTCAGCAACAACAGCCTGAGC AGCAATGCCAGCCTCCCCAGCGTGCAGAGCTGCCGGCGCCTGCGTGAGAGGAGGGTCGCCAGCTGGGCC GTGTCCTTTGAGCGCCTGCTGCAGGACCCCGTCGGTGTCCGCTACTTCTCTGATTTTCTAAGGAAAGAA TTCAGTGAAGAAAACATTTTATTCTGGCAGGCCTGTGAATATTTTAATCATGTTCCTGCACATGACAAA AAGGAGCTTTCCTACAGGGCCCGGGAGATTTTCAGTAAGTTTCTCTGCAGCAAAGCCACCACCCCGGTC AACATCGACAGCCAGGCCCAGCTAGCAGACGACGTCCTCCGCGCACCTCACCCAGACATGTTCAAGGAG CAGCAGCTGCAGATCTTCAATCTCATGAAGTTTGATAGCTACACTCGCTTTCTGAAGTCCCCGCTGTAC CAGGAATGCATCCTGGCGGAAGTGGAGGGCCGTGCACTCCCGGACTCGCAGCAGGTCCCCAGCAGCCCG GCTTCCAAGCACAGCCTCGGTTCAGACCACTCCAGTGTGTCCACGCCAAAAAAGTTAAGTGGAAAATCA AAATCCGGCCGATCCCTGAATGAAGAGCTGGGGGATGAGGACAGCGAGAAGAAGCGGAAAGGCGCGTTT TTCTCGTGGTCGCGGACCAGGAGCACCGGGAGGTCCCAGAAAAAGAGGGAGCACGGGGACCACGCAGAC GACGCCCTGCATGCCAATGGAGGCCTGTGTCGCCGAGAGTCGCAGGGCTCTGTGTCCTCTGCGGGGAGC CTGGACCTGTCGGAGGCCTGCAGGACTTTGGCACCCGAGAAGGACAAGGCCACCAAGCACTGCTGCATT CATCTCCCGGATGGGACATCCTGCGTGGTGGCTGTCAAGGCGGGCTTCTCCATCAAAGACATCCTGTCC GGACTCTGTGAGCGGCATGGCATCAACGGGGCGGCCGCGGACCTCTTCCTGGTGGGCGGGGACAAGCCT CTGGTGCTGCACCAAGACAGTAGCATCTTGGAGTCAAGGGACCTGCGCCTAGAAAAGCGCACCTTGTTT CGGCTGGATCTTGTTCCGATTAACCGGTCAGTGGGACTCAAGGCCAAGCCCACCAAGCCCGTCACGGAG GTGCTGCGGCCCGTGGTGGCCAGATACGGCCTGGACCTCAGTGGCCTGCTGGTGAGGCTGAGTGGAGAG AAGGAGCCCCTGGACCTTGGCGCCCCTATATCGAGTCTGGACGGACAGCGGGTTGTCTTGGAGGAGAAG GATCCTTCCAGAGGAAAGGCATCCGCAGATAAACAGAAAGGTGTGCCAGTGAAACAGAACACAGCTGTA AATTCCAGCTCCAGAAACCACTCGGCTACGGGAGAGGAAAGAACACTAGGCAAGTCTAATTCTATTAAA ATAAAAGGAGAAAATGGAAAAAATGCTAGGGATCCCCGGCTTTCAAAGAGAGAAGAATCTATTGCAAAG ATTGGGAAAAAAAAATATCAGAAAATTAATTTGGACGAAGCAGAGGAGTTTTTTGAGCTTATTTCCAAA GCTCAGAGCAACAGAGCAGATGACCAACGTGGGCTGCTAAGGAAGGAAGACCTGGTGTTGCCAGAGTTC CTCCGTTTACCTCCTGGTTCCACAGAACTCACCCTCCCCACTCCAGCTGCTGTGGCCAAGGGCTTTAGC AAGAGAAGCGCCACAGGCAACGGCCGGGAGAGCGCCTCCCAGCCTGGCGAGCAGTGGGAGCCAGTCCAG GAGAGCAGCGACAGCCCGTCCACCAGCCCGGGCTCAGCCTCCAGCCCCCCTGGACCTCCTGGGACGACC CCCCCCGGGCAGAAGTCTCCCAGCGGGCCCTTCTGCACTCCCCAGTCCCCCGTCTCCCTCGCGCAGGAG GGCACCGCCCAGATCTGGAAGAGGCAGTCTCAGGAAGTGGAGGCCGGGGGCATCCAGACGGTGGAGGAT GAGCACGTGGCCGAGCTGACCCTGATGGGGGAGGGGGACATCAGCAGCCCCAACAGCACCTTGCTGCCG CCGCCCTCCACCCCCCAGGAAGTGCCAGGACCTTCCAGACCAGGAAGTGGGACCCATGGCAGCCGAGAC CTCCCAGTCAACAGAATCATCGATGTGGATCTTGTAACTGGCTCGGCGCCCGGGCGGGATGGTGGCATA GCGGGGGCACAGGCTGGCCCTGGGAGGTCGCAGGCCAGTGGTGGGCCTCCTACATCAGACCTCCCTGGC TTGGGCCCCGTCCCGGGTGAGCCTGCTAAGCCCAAGACCAGCGCTCACCACGCCACCTTCGTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_198229 |
| Insert Size | 4344 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_198229.2 |
| RefSeq Size | 5486 bp |
| RefSeq ORF | 4344 bp |
| Locus ID | 6002 |
| UniProt ID | O14924 |
| Protein Families | Druggable Genome |
| MW | 156.4 kDa |
| Gene Summary | This gene encodes a member of the 'regulator of G protein signaling' (RGS) gene family. The encoded protein may function as a guanosine triphosphatase (GTPase)-activating protein as well as a transcriptional repressor. This protein may play a role in tumorigenesis. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their biological nature has not been determined. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (1) encodes the longest isoform (1). |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224508 | RGS12 (Myc-DDK-tagged)-Human regulator of G-protein signaling 12 (RGS12), transcript variant 1 |
CNY 14848.00 |
|
| RC224508L1 | Lenti ORF clone of Human regulator of G-protein signaling 12 (RGS12), transcript variant 1, Myc-DDK-tagged |
CNY 12730.00 |
|
| RC224508L2 | Lenti ORF clone of Human regulator of G-protein signaling 12 (RGS12), transcript variant 1, mGFP tagged |
CNY 17248.00 |
|
| RC224508L3 | Lenti ORF clone of Human regulator of G-protein signaling 12 (RGS12), transcript variant 1, Myc-DDK-tagged |
CNY 12730.00 |
|
| RC224508L4 | Lenti ORF clone of Human regulator of G-protein signaling 12 (RGS12), transcript variant 1, mGFP tagged |
CNY 12730.00 |
|
| RG224508 | RGS12 (tGFP-tagged) - Human regulator of G-protein signaling 12 (RGS12), transcript variant 1 |
CNY 11880.00 |
