SERCA3 (ATP2A3) (NM_174953) Human Untagged Clone
CAT＃: SC309449
ATP2A3 (untagged)-Human ATPase, Ca++ transporting, ubiquitous (ATP2A3), transcript variant 5
CNY 7336.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SERCA3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC309449 representing NM_174953.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGGCGGCGCATCTGCTCCCGGCCGCCGACGTGCTGCGCCACTTCTCGGTGACAGCCGAGGGCGGC CTGAGCCCGGCGCAGGTGACCGGCGCGCGGGAGCGCTACGGCCCCAACGAGCTCCCGAGTGAGGAAGGG AAGTCCCTGTGGGAGCTGGTGCTGGAACAGTTTGAGGACCTCCTGGTGCGCATCCTGCTGCTGGCTGCC CTTGTCTCCTTTGTCCTGGCCTGGTTCGAGGAGGGCGAGGAGACCACGACCGCCTTCGTGGAGCCCCTG GTCATCATGCTGATCCTCGTGGCCAACGCCATTGTGGGCGTGTGGCAGGAACGCAACGCCGAGAGTGCC ATCGAGGCCCTGAAGGAGTATGAGCCTGAGATGGGCAAGGTGATCCGCTCGGACCGCAAGGGCGTGCAG AGGATCCGTGCCCGGGACATCGTCCCAGGGGACATTGTAGAAGTGGCAGTGGGGGACAAAGTGCCTGCT GACCTCCGCCTCATCGAGATCAAGTCCACCACGCTGCGAGTGGACCAGTCCATCCTGACGGGTGAATCT GTGTCCGTGACCAAGCACACAGAGGCCATCCCAGACCCCAGAGCTGTGAACCAGGACAAGAAGAACATG CTGTTTTCTGGCACCAATATCACATCGGGCAAAGCGGTGGGTGTGGCCGTGGCCACCGGCCTGCACACG GAGCTGGGCAAGATCCGGAGCCAGATGGCGGCAGTCGAGCCCGAGCGGACGCCGCTGCAGCGCAAGCTG GACGAGTTTGGACGGCAGCTGTCCCACGCCATCTCTGTGATCTGCGTGGCCGTGTGGGTCATCAACATC GGCCACTTCGCCGACCCGGCCCACGGTGGCTCCTGGCTGCGTGGCGCTGTCTACTACTTCAAGATCGCC GTGGCCCTGGCGGTGGCGGCCATCCCCGAGGGCCTCCCGGCTGTCATCACTACATGCCTGGCACTGGGC ACGCGGCGCATGGCACGCAAGAACGCCATCGTGCGAAGCCTGCCGTCCGTGGAGACCCTGGGCTGCACC TCAGTCATCTGCTCCGACAAGACGGGCACGCTCACCACCAATCAGATGTCTGTCTGCCGGATGTTCGTG GTAGCCGAGGCCGATGCGGGCTCCTGCCTTTTGCACGAGTTCACCATCTCGGGTACCACGTATACCCCC GAGGGCGAAGTGCGGCAGGGGGATCAGCCTGTGCGCTGCGGCCAGTTCGACGGGCTGGTGGAGCTGGCG ACCATCTGCGCCCTGTGCAACGACTCGGCTCTGGACTACAACGAGGCCAAGGGTGTGTATGAGAAGGTG GGAGAGGCCACGGAGACAGCTCTGACTTGCCTGGTGGAGAAGATGAACGTGTTCGACACCGACCTGCAG GCTCTGTCCCGGGTGGAGCGAGCTGGCGCCTGTAACACGGTCATCAAGCAGCTGATGCGGAAGGAGTTC ACCCTGGAGTTCTCCCGAGACCGGAAATCCATGTCCGTGTACTGCACGCCCACCCGCCCTCACCCTACT GGCCAGGGCAGCAAGATGTTTGTGAAGGGGGCTCCTGAGAGTGTGATCGAGCGCTGTAGCTCAGTCCGC GTGGGGAGCCGCACAGCACCCCTGACCCCCACCTCCAGGGAGCAGATCCTGGCAAAGATCCGGGATTGG GGCTCAGGCTCAGACACGCTGCGCTGCCTGGCACTGGCCACCCGGGACGCGCCCCCAAGGAAGGAGGAC ATGGAGCTGGACGACTGCAGCAAGTTTGTGCAGTACGAGACGGACCTGACCTTCGTGGGCTGCGTAGGC ATGCTGGACCCGCCGCGACCTGAGGTGGCTGCCTGCATCACACGCTGCTACCAGGCGGGCATCCGCGTG GTCATGATCACGGGGGATAACAAAGGCACTGCCGTGGCCATCTGCCGCAGGCTTGGCATCTTTGGGGAC ACGGAAGACGTGGCGGGCAAGGCCTACACGGGCCGCGAGTTTGATGACCTCAGCCCCGAGCAGCAGCGC CAGGCCTGCCGCACCGCCCGCTGCTTCGCCCGCGTGGAGCCCGCACACAAGTCCCGCATCGTGGAGAAC CTGCAGTCCTTTAACGAGATCACTGCTATGACTGGCGATGGAGTGAACGACGCACCAGCCCTGAAGAAA GCAGAGATCGGCATCGCCATGGGCTCAGGCACGGCCGTGGCCAAGTCGGCGGCAGAGATGGTGCTGTCA GATGACAACTTTGCCTCCATCGTGGCTGCGGTGGAGGAGGGCCGGGCCATCTACAGCAACATGAAGCAA TTCATCCGCTACCTCATCTCCTCCAATGTTGGCGAGGTCGTCTGCATCTTCCTCACGGCAATTCTGGGC CTGCCCGAAGCCCTGATCCCTGTGCAGCTGCTCTGGGTGAACCTGGTGACAGACGGCCTACCTGCCACG GCTCTGGGCTTCAACCCGCCAGACCTGGACATCATGGAGAAGCTGCCCCGGAGCCCCCGAGAAGCCCTC ATCAGTGGCTGGCTCTTCTTCCGATACCTGGCTATCGGAGTGTACGTAGGCCTGGCCACAGTGGCTGCC GCCACCTGGTGGTTTGTGTATGACGCCGAGGGACCTCACATCAACTTCTACCAGCTGAGGAACTTCCTG AAGTGCTCCGAAGACAACCCGCTCTTTGCCGGCATCGACTGTGAGGTGTTCGAGTCACGCTTCCCCACC ACCATGGCCTTGTCCGTGCTCGTGACCATTGAAATGTGCAATGCCCTCAACAGCGTCTCGGAGAACCAG TCGCTGCTGCGGATGCCGCCCTGGATGAACCCCTGGCTGCTGGTGGCTGTGGCCATGTCCATGGCCCTG CACTTCCTCATCCTGCTCGTGCCGCCCCTGCCTCTCATTTTCCAGGTGACCCCACTGAGCGGGCGCCAG TGGGTGGTGGTGCTCCAGATATCTCTGCCTGTCATCCTGCTGGATGAGGCCCTCAAGTACCTGTCCCGG AACCACATGCACGCCTGTCTTTATCCAGGCCTTCTCAGGACAGTCTCGCAGGCCTGGAGTAGGCAGCCG CTGACCACCTCTTGGACCCCAGACCACACCGGGTTGGCCTCTTTGGGCCAGGGACACAGCATCGTCTCG CTGTCAGAGCTGCTCAGAGAGGGAGGAAGCAGGGAAGAAATGAGCCAGAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_174953 |
| Insert Size | 3159 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_174953.1 |
| RefSeq Size | 4898 bp |
| RefSeq ORF | 3159 bp |
| Locus ID | 489 |
| UniProt ID | Q93084 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Alzheimer's disease, Calcium signaling pathway |
| MW | 114.9 kDa |
| Gene Summary | This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (5) includes two additional exons in the 3' coding region but maintains the reading frame, compared to variant 1. Variant 5 encodes isoform e, which is longer than isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217328 | ATP2A3 (Myc-DDK-tagged)-Human ATPase, Ca++ transporting, ubiquitous (ATP2A3), transcript variant 5 |
CNY 7328.00 |
|
| RC217328L3 | Lenti ORF clone of Human ATPase, Ca++ transporting, ubiquitous (ATP2A3), transcript variant 5, Myc-DDK-tagged |
CNY 12350.00 |
|
| RC217328L4 | Lenti ORF clone of Human ATPase, Ca++ transporting, ubiquitous (ATP2A3), transcript variant 5, mGFP tagged |
CNY 12350.00 |
|
| RG217328 | ATP2A3 (tGFP-tagged) - Human ATPase, Ca++ transporting, ubiquitous (ATP2A3), transcript variant 5 |
CNY 11500.00 |
