DOCK7 (NM_033407) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DEE23; EIEE23; ZIR2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC309820 representing NM_033407. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCGAGCGCCGCGCCTTCGCCCAGAAGATCAGCAGAACGGTGGCAGCCGAAGTTAGGAAGCAGATC TCCGGACAATATAGTGGTTCTCCCCAACTGCTCAAAAACCTTAATATTGTTGGCAATATATCCCATCAC ACCACAGTGCCCCTTACCGAAGCAGTAGATCCAGTGGATTTGGAAGATTACCTCATTACTCATCCTTTG GCTGTGGATTCTGGGCCTTTACGGGATTTGATTGAATTTCCTCCAGATGATATTGAAGTTGTTTATAGT CCTCGGGACTGCAGAACTCTTGTTTCAGCTGTACCTGAAGAAAGTGAAATGGATCCACATGTTAGAGAC TGTATAAGAAGTTATACAGAAGACTGGGCAATTGTCATCAGAAAATATCATAAATTGGGAACAGGATTT AATCCCAATACATTAGATAAACAGAAAGAAAGGCAAAAAGGTTTGCCAAAACAAGTTTTTGAATCTGAT GAAGCTCCAGATGGCAACAGCTACCAGGATGATCAAGATGACCTTAAAAGACGTTCAATGTCAATAGAT GATACCCCAAGGGGTAGCTGGGCCTGTAGTATCTTTGACTTGAAAAATTCACTTCCTGATGCTTTGCTT CCCAATTTACTTGATCGAACTCCAAATGAAGAAATAGACCGTCAGAATGATGACCAAAGGAAATCAAAC CGTCACAAAGAACTTTTTGCTTTGCATCCATCACCAGATGAGGAAGAACCAATAGAACGGCTTAGTGTT CCTGATATACCCAAAGAACATTTTGGTCAAAGACTTCTTGTAAAATGCTTATCACTCAAGTTTGAAATT GAAATTGAACCCATTTTTGCAAGTTTGGCTTTATATGATGTCAAGGAAAAGAAAAAGATTTCAGAAAAC TTTTATTTTGACCTTAATTCTGAGCAGATGAAAGGGTTGTTACGTCCACATGTACCACCTGCTGCCATT ACTACCCTGGCAAGATCAGCAATTTTTTCTATCACTTATCCTTCCCAAGATGTTTTTCTTGTAATAAAG CTAGAAAAAGTCCTACAGCAAGGAGACATTGGAGAGTGTGCAGAACCATATATGATTTTCAAAGAAGCA GATGCCACCAAGAATAAAGAAAAACTGGAGAAACTGAAGAGTCAAGCAGATCAGTTTTGCCAAAGACTT GGGAAATATCGCATGCCTTTTGCTTGGACTGCAATCCATTTAATGAATATTGTTAGCAGTGCTGGGAGT TTGGAAAGAGATTCTACAGAAGTAGAAATCAGTACTGGAGAACGAAAAGGGTCTTGGTCAGAGAGGAGG AATTCTAGTATTGTTGGCAGACGATCACTTGAAAGGACAACAAGTGGAGATGATGCTTGTAACTTGACG AGCTTTCGACCAGCTACTCTCACAGTGACAAATTTTTTTAAGCAGGAAGGAGACCGCTTAAGTGATGAA GATCTCTACAAATTCCTTGCTGATATGAGAAGGCCATCTTCTGTCTTACGGCGACTAAGACCTATTACA GCTCAGCTCAAGATAGACATTTCTCCCGCACCTGAAAATCCCCATTATTGCCTAACTCCGGAGCTGCTT CAAGTGAAGCTTTACCCTGACAGTAGAGTTAGACCTACCAGAGAAATCTTAGAGTTTCCCGCAAGGGAT GTTTATGTTCCAAACACTACTTACAGAAATCTTCTCTACATATACCCTCAGAGTCTTAATTTTGCCAAT CGTCAAGGTTCTGCTAGAAATATAACAGTGAAAGTCCAGTTTATGTATGGAGAGGATCCAAGCAATGCC ATGCCGGTAATCTTTGGTAAATCTAGCTGTTCAGAATTTTCAAAGGAAGCCTATACAGCCGTAGTATAT CATAACAGGTCTCCTGATTTTCATGAAGAAATCAAGGTTAAGCTTCCTGCTACTTTAACTGACCATCAT CACTTGCTTTTTACTTTTTATCATGTTAGTTGTCAACAAAAACAAAATACTCCTCTTGAAACACCAGTT GGATATACATGGATACCAATGCTTCAGAATGGACGGTTGAAGACTGGCCAGTTTTGCTTGCCAGTCTCA TTGGAAAAACCACCACAGGCTTATTCTGTACTGTCTCCTGAGGTTCCTCTACCTGGCATGAAATGGGTA GATAATCACAAAGGTGTTTTTAATGTTGAAGTTGTTGCTGTTTCGTCTATCCATACACAAGATCCTTAT CTTGACAAATTTTTTGCTCTGGTCAATGCTCTGGATGAACACCTGTTCCCAGTCCGAATTGGGGACATG CGAATCATGGAAAATAACTTAGAAAATGAATTGAAGAGCAGTATTTCAGCACTGAATTCATCCCAGCTG GAACCAGTGGTCCGATTTCTTCATCTTCTGCTAGATAAACTGATACTTTTAGTTATTAGACCTCCTGTC ATTGCTGGCCAAATAGTTAACCTAGGTCAAGCATCTTTTGAAGCCATGGCATCAATTATAAATCGACTT CACAAAAACTTGGAAGGAAATCATGACCAGCATGGCAGAAACAGCCTTCTTGCATCATATATTCATTAT GTTTTCCGCCTACCAAATACTTACCCTAATTCATCATCACCAGGTCCTGGGGGTTTGGGAGGATCAGTG CATTATGCCACAATGGCTAGATCTGCGGTGAGACCTGCAAGCCTTAATTTAAATCGTTCTCGAAGCCTT AGTAATAGCAATCCAGATATATCTGGGACTCCCACGTCACCAGATGATGAAGTTCGATCAATCATCGGG AGTAAGGCTATGGATCGAAGTTGTAATCGTATGTCTTCGCACACAGAGACGTCAAGTTTCTTACAAACA TTAACGGGACGCTTACCAACTAAAAAGCTTTTTCACGAGGAGCTGGCTTTGCAGTGGGTTGTTTGCAGT GGCAGCGTTCGGGAATCAGCTTTGCAACAAGCCTGGTTCTTTTTTGAATTAATGGTAAAGAGCATGGTG CACCATTTATACTTTAATGATAAACTTGAGGCTCCAAGGAAAAGTCGTTTTCCAGAACGTTTCATGGAT GACATTGCAGCTCTTGTCAGCACGATTGCTAGTGATATAGTTTCACGATTTCAGAAGGACACAGAAATG GTTGAGAGACTCAATACAAGCCTTGCATTCTTTCTCAATGATCTGTTGTCTGTTATGGACAGAGGATTT GTTTTTAGCCTTATAAAGTCCTGCTATAAACAGGTGTCTTCAAAGCTTTACTCATTACCGAATCCCAGT GTTCTGGTGTCCTTGAGGCTGGATTTTCTACGAATCATCTGCAGTCATGAGCACTATGTTACATTAAAC TTACCCTGCAGCTTACTTACTCCACCTGCATCTCCATCACCTTCTGTTTCTTCTGCAACATCTCAGAGT TCTGGATTTTCTACGAATGTACAAGACCAAAAGATTGCAAATATGTTTGAATTATCCGTGCCTTTCCGC CAACAGCATTATTTGGCAGGACTTGTGTTAACAGAGCTGGCTGTCATTTTAGACCCTGATGCTGAAGGA CTGTTTGGATTGCATAAGAAAGTCATCAATATGGTACACAATTTACTCTCCAGTCACGACTCAGACCCG CGGTACTCTGACCCTCAGATAAAGGCTCGAGTGGCCATGTTGTATCTACCTCTGATTGGTATTATCATG GAAACTGTACCTCAGCTGTATGATTTTACAGAAACTCACAATCAACGAGGAAGACCAATTTGTATAGCC ACTGATGATTATGAAAGTGAGAGCGGAAGTATGATAAGCCAGACCGTTGCCATGGCAATCGCAGGGACA TCGGTCCCTCAACTAACAAGGCCTGGCAGTTTCCTCCTCACGTCAACGAGTGGCAGGCAACACACTACC TTTTCAGCAGAATCAAGTCGAAGCCTTTTGATCTGTCTACTTTGGGTTCTCAAAAATGCAGATGAAACA GTTCTACAGAAGTGGTTTACAGATCTCTCAGTCTTGCAGCTAAACCGGCTATTAGATCTGCTTTATCTC TGTGTGTCTTGCTTTGAGTATAAAGGGAAAAAAGTGTTTGAACGAATGAATAGCTTGACCTTTAAGAAA TCAAAAGACATGAGAGCAAAGCTTGAAGAAGCTATTCTTGGGAGCATAGGTGCCAGGCAAGAAATGGTA CGGCGAAGCCGAGGACAGCTCGGTACGTACACAATAGCTTCTCCTCCTGAGAGAAGCCCATCTGGAAGT GCCTTTGGAAGTCAAGAAAATTTGAGGTGGAGGAAAGATATGACTCACTGGCGTCAAAACACAGAGAAG CTTGACAAATCAAGAGCAGAGATTGAACACGAAGCACTGATTGATGGAAACCTGGCTACAGAAGCAAAC CTAATCATTTTAGATACATTAGAGATTGTTGTTCAGACCGTTTCTGTAACGGAATCCAAAGAGAGCATT CTTGGTGGAGTGCTAAAAGTGCTACTACACAGCATGGCCTGTAACCAAAGTGCAGTTTATCTACAACAC TGTTTTGCTACACAGAGAGCCTTGGTTTCAAAGTTTCCTGAACTCTTATTTGAAGAAGAGACAGAGCAG TGTGCTGATTTATGCCTCAGGCTTCTCCGACACTGTAGCAGTAGCATCGGTACAATACGGTCACACGCC AGTGCCTCCCTTTACCTACTAATGAGGCAAAACTTTGAGATTGGGAATAACTTTGCCAGGGTTAAAATG CAGGTAACAATGTCACTATCCTCCTTGGTGGGCACATCTCAGAATTTTAATGAAGAATTCTTAAGACGT TCTCTAAAGACTATATTGACATATGCTGAAGAAGATCTGGAATTGAGGGAAACAACATTTCCTGATCAG GTCCAGGATCTGGTTTTCAATCTCCATATGATTCTTTCTGATACTGTGAAAATGAAGGAACACCAGGAG GATCCTGAAATGTTGATTGATCTAATGTACAGAATTGCCAAGGGTTACCAGACCTCTCCAGATCTGCGA TTGACCTGGTTGCAGAACATGGCAGGCAAGCACTCAGAACGAAGCAATCATGCTGAAGCTGCACAGTGT CTAGTCCACTCAGCAGCACTTGTTGCTGAATATTTGAGCATGCTGGAGGACCGGAAATATCTTCCTGTG GGATGTGTAACATTTCAGAATATTTCATCTAATGTTTTAGAAGAATCTGCGGTCTCAGATGATGTGGTA TCTCCAGATGAAGAAGGTATCTGCTCTGGAAAATACTTTACTGAGTCAGGACTTGTGGGATTACTGGAA CAAGCAGCTGCTTCCTTCTCTATGGCTGGCATGTATGAAGCAGTTAATGAAGTTTACAAAGTACTTATT CCTATTCATGAAGCTAATCGGGATGCAAAGAAACTATCCACAATTCATGGTAAACTTCAAGAAGCATTC AGCAAAATTGTTCATCAGAGTACTGGCTGGGAGCGGATGTTTGGCACCTATTTTCGTGTTGGTTTTTAT GGAACCAAGTTCGGGGATTTGGATGAACAAGAATTTGTTTACAAGGAGCCTGCAATAACCAAACTTGCA GAGATATCTCACAGATTGGAGGGATTTTACGGAGAAAGATTTGGAGAGGATGTGGTTGAAGTAATCAAA GACTCTAATCCTGTAGACAAGTGTAAATTAGATCCTAACAAGGCATATATTCAGATTACCTATGTGGAG CCATACTTTGACACATATGAGATGAAGGACAGAATCACCTATTTCGACAAAAATTACAATCTTCGTCGA TTCATGTACTGTACACCCTTTACTTTAGATGGCCGTGCCCATGGGGAACTTCATGAACAATTCAAAAGG AAGACCATTCTGACTACGTCTCATGCCTTTCCTTATATTAAAACAAGGGTCAATGTCACTCATAAAGAA GAGATCATCTTAACACCAATTGAAGTTGCTATTGAGGACATGCAGAAAAAGACACAGGAGTTGGCATTT GCAACACATCAGGATCCCGCAGACCCCAAAATGCTTCAGATGGTACTCCAGGGATCTGTAGGCACCACA GTGAATCAGGGGCCTTTGGAAGTTGCCCAGGTTTTTCTGTCTGAAATACCTAGTGACCCAAAGCTCTTC AGACATCATAATAAACTGCGACTCTGCTTTAAAGATTTTACTAAAAGGTGTGAAGATGCCTTAAGAAAA AATAAGAGCTTAATTGGGCCGGATCAAAAGGAGTATCAAAGGGAACTGGAGAGAAACTATCATCGCCTT AAAGAGGCCCTACAGCCACTGATCAACAGAAAGATCCCTCAGTTATACAAGGCAGTATTGCCTGTCACC TGCCACAGAGATTCCTTCAGTCGAATGAGCCTTCGCAAAATGGATCTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_033407
Insert Size	6330 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_033407.3
RefSeq Size	7133 bp
RefSeq ORF	6330 bp
Locus ID	85440
UniProt ID	Q96N67
MW	239.4 kDa
Gene Summary	The protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that plays a role in axon formation and neuronal polarization. The encoded protein displays GEF activity toward RAC1 and RAC3 Rho small GTPases but not toward CDC42. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012] Transcript Variant: This variant (2) lacks an alternate in-frame exon and uses an alternate in-frame splice junction compared to variant 6. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 6.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.