ISM2 (NM_182509) Human Untagged Clone

CAT#: SC312830

ISM2 (untagged)-Human isthmin 2 homolog (zebrafish) (ISM2), transcript variant 3



  "NM_182509" in other vectors (4)

CNY 6270.00


货期*
4周

规格
    • 10 ug

Product images

推荐一起购买 (4)
Rabbit Polyclonal Anti-ISM2 Antibody
    • 100 ul

CNY 1999.00
CNY 3280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms TAIL1; THSD3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC312830 representing NM_182509.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGCGTGCGCTCCGCGACCGAGCCGGGCTCCTCCTCTGCGTGCTGCTGCTGGCGGCGCTGCTGGAGGCG
GCGCTAGGGCTCCCCGTGAAGAAGCCGCGGCTCCGCGGACCACGGCCTGGGAGCCTCACGAGGCTCGCA
GAGGTCTCAGCCTCCCCAGATCCTAGGCCTCTGAAGGAAGAGGAGGAGGCACCACTGCTCCCCAGAACC
CACCTGCAGGCAGAGCCACACCAACATGGATGCTGGACTGTCACTGAGCCAGCAGCCATGACCCCAGGC
AACGCCACCCCTCCCAGGACCCCAGAGGTTACTCCGTTGCGGCTGGAGCTGCAGAAGCTGCCGGGATTG
GCCAACACAACCTTGAGTACCCCTAACCCTGATACCCAGGCTTCAGCCTCCCCAGATCCTAGGCCTCTG
AGGGAAGAGGAGGAGGCACGACTGCTCCCCAGAACCCACCTGCAGGCAGAGCTACACCAACATGGATGT
TGGACTGTCACTGAGCCAGCAGCCCTGACCCCAGGGAATGCCACGCCTCCCAGGACCCAGGAGGTTACT
CCCTTGCTGCTGGAGCTGCAGAAGCTGCCAGAATTGGTCCACGCAACCTTGAGTACCCCTAACCCTGAT
AACCAGACTATGAGCCTCAGAAGGAGTGGAGTCCCTGGTCTCCCTGCAGTGGGAACTGCAGCACTGGCA
AGCAGCAGAGGACTCGGCCCTGTGGCTATGGCTGCACTGCCACCGAGACCCGTACCTGTGACCTGCCCT
CCTGTCCTGGCACTGAGGACAAGGACACCTTGGGCCTCCCCAGTGAGGAGTGGAAGCTCCTGGCCCGCA
ATGCTACGGACATGCATGATCAAGATGTGGACAGCTGTGAGAAGTGGCTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_182509
Insert Size 879 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_182509.3
RefSeq Size 2660 bp
RefSeq ORF 879 bp
Locus ID 145501
UniProt ID Q6H9L7
Protein Families Transmembrane
MW 31.3 kDa
Gene Summary The protein encoded by this gene contains a type 1 thrombospondin domain, which is present in thrombospondin, a number of proteins involved in the complement pathway, as well as in extracellular matrix proteins. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Mar 2009]
Transcript Variant: This variant (3) lacks an in-frame coding exon compared to variant 1. The resulting isoform (3) thus lacks an internal segment compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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