RFFL (NM_057178) Human Untagged Clone
CAT＃: SC313138
RFFL (untagged)-Human ring finger and FYVE-like domain containing 1 (RFFL), transcript variant 1
CNY 5990.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CARP2; fring; FYVE-RING finger protein SAKURA; RIFIFYLIN; ring finger and FYVE-like domain containing 1; RNF34L; RNF189 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF sequence for NM_057178 edited
ATGTGGGCAACCTGCTGCAACTGGTTCTGCCTGGATGGACAGCCTGAGGAGGTCCCACCA CCCCAGGGAGCCAGGATGCAGGCCTATTCCAACCCTGGGTACAGCTCCTTCCCTTCCCCA ACAGGCTTGGAACCAAGCTGCAAGTCCTGTGGGGCTCACTTTGCAAACACGGCCAGGAAG CAGACCTGCTTGGACTGTAAGAAAAATTTTTGCATGACCTGTTCGAGCCAAGTAGGGAAT GGGCCCCGCCTCTGCCTTCTCTGCCAACGGTTTCGAGCTACAGCCTTTCAGCGAGAGGAG CTCATGAAGATGAAGGTGAAGGACTTGAGGGACTATCTCAGCCTCCATGACATCTCTACC GAAATGTGCCGGGAGAAAGAAGAGCTGGTGCTCTTGGTCCTTGGCCAGCAGCCTGTAATC TCCCAGGAGGACAGGACTCGTGCCTCCACCTTGTCCCCAGACTTTCCTGAGCAGCAGGCC TTCCTGACCCAGCCTCACTCCAGCATGGTTCCACCTACCTCACCCAACCTCCCCTCTTCA TCTGCACAAGCCACCTCTGTTCCCCCAGCCCAGGTTCAGGAGAATCAGCAGGCCAATGGC CATGTGTCTCAGGATCAAGAGGAACCCGTCTACCTGGAGAGCGTGGCCAGAGTACCTGCT GAGGATGAGACCCAGTCTATTGACTCAGAGGACAGCTTTGTCCCAGGCCGAAGGGCCTCT CTGTCTGACCTGACTGACCTGGAGGACATTGAAGGCCTGACAGTGCGGCAGCTGAAAGAG ATCTTGGCTCGCAACTTTGTCAACTACAAGGGCTGCTGTGAGAAGTGGGAGCTGATGGAG AGAGTGACCCGGCTATACAAGGATCAGAAAGGACTCCAGCACCTGGTCAGTGGTGCCGAA GACCAAAACGGGGGAGCAGTACCATCAGGCTTGGAGGAGAACCTGTGTAAGATCTGCATG GACTCACCCATTGACTGTGTTCTTCTGGAGTGTGGCCACATGGTAACCTGTACCAAGTGT GGCAAGCGCATGAATGAATGTCCCATCTGCCGGCAGTATGTAATCCGAGCTGTGCATGTC TTCCGGTCCTGA >OriGene 5' read for NM_057178 unedited
TTCAAATTTTGTAATACGAACTCACTATAGGGCGGCCGCGAATTCGCACGAGGGAGGGCG GCCGCAGCAGATTTTATCATGTGGGCAACCTGCTGCAACTGGTTCTGCCTGGATGGACAG CCTGAGGAGGTCCCACCACCCCAGGGAGCCAGGATGCAGGCCTATTCCAACCCTGGGTAC AGCTCCTTCCCTTCCCCAACAGGCTTGGAACCAAGCTGCAAGTCCTGTGGGGCTCACTTT GCAAACACGGCCAGGAAGCAGACCTGCTTGGACTGTAAGAAAAATTTTTGCATGACCTGT TCGAGCCAAGTAGGGAATGGGCCCCGCCTCTGCCTTCTCTGCCAACGGTTTCGAGCTACA GCCTTTCAGCGAGAGGAGCTCATGAAGATGAAGGTGAAGGACTTGAGGGACTATCTCAGC CTCCATGACATCTCTACCGAAATGTGCCGGGAGAAAGAAGAGCTGGTGCTCTTGGTCCTT GGCCAGCAGCCTGTAATCTCCCAGGAGGACAGGACTCGTGCCTCCACCTTGTCCCCAGAC TTTCCTGAGCAGCAGGCCTTCCTGACCCAGCCTCACTCCAGCATGGTTCCACCTACCTCA CCCAACCTCCCCTCTTCATCTGCACAAGCCACCTCTGTTCCCCCAGCCCAGGTTCAGGAG AATCAGCAGGCCAATGGCCATGTGTCTCAAGATCAAGAGGAACCCGTCTACCTGGAGAGC GTGGCCAGAGTACCTGCTGAGGATGAGACCCAGTCTATTGACTNCAGAGACAGCTTTGTC CCCAGCCCGAAGGCCTCTCTGTCTGACCTGACTGACCTGNNAGACATTGAANGCCTGACA GTGCNGCAGCTGAAAGAGATCTGGCTCGCAACTTGTCACTACAGGNCTGCTGTGAAAATG GGACTGATGGANAGATGACCCGCTTACAAGATCAGAAGACTNCGACCTGGTCATGGTGCG AGACAAAACGGGAA |
| Restriction Sites | Please inquire |
| ACCN | NM_057178 |
| Insert Size | 5500 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to be a perfect match to NM_057178.3. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_057178.3, NP_476519.1 |
| RefSeq Size | 4143 bp |
| RefSeq ORF | 1092 bp |
| Locus ID | 117584 |
| Protein Families | Druggable Genome |
| Gene Summary | E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby indirectly regulating cell migration through the mTORC2 complex. Ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate cell death downstream of death domain receptors in the extrinsic pathway of apoptosis. Negatively regulates the tumor necrosis factor-mediated signaling pathway through targeting of RIPK1 to ubiquitin-mediated proteasomal degradation. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. May also play a role in endocytic recycling.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Both variants encode the same protein. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC212645 | RFFL (Myc-DDK-tagged)-Human ring finger and FYVE-like domain containing 1 (RFFL), transcript variant 1 |
CNY 3656.00 |
|
| RC212645L3 | Lenti ORF clone of Human ring finger and FYVE-like domain containing 1 (RFFL), transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC212645L4 | Lenti ORF clone of Human ring finger and FYVE-like domain containing 1 (RFFL), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RG212645 | RFFL (tGFP-tagged) - Human ring finger and FYVE-like domain containing 1 (RFFL), transcript variant 1 |
CNY 4370.00 |
