KLHL24 (NM_017644) Human Untagged Clone
CAT＃: SC314153
KLHL24 (untagged)-Human kelch-like 24 (Drosophila) (KLHL24)
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DRE1; EBSSH; KRIP6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC314153 representing NM_017644.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTACTAATATTGGGACGCAGACTAAACAGAGAGGATCTTGGGGTGCGTGATTCCCCAGCAACTAAG CGAAAAGTTTTTGAAATGGACCCCAAATCTCTGACAGGTCATGAGTTTTTTGACTTCTCTTCAGGATCA TCCCATGCCGAAAACATACTCCAGATATTTAATGAATTTCGTGATAGCCGCTTATTCACAGATGTTATC ATTTGTGTGGAAGGAAAAGAATTTCCTTGCCATAGAGCTGTGCTCTCAGCCTGTAGCAGCTACTTCAGA GCTATGTTTTGTAATGACCACAGGGAAAGCCGAGAAATGTTGGTTGAGATCAATGGTATTTTAGCTGAA GCTATGGAATGTTTTTTGCAGTATGTTTATACTGGAAAGGTGAAGATCACTACAGAGAATGTACAGTAT CTCTTTGAGACATCAAGCCTCTTTCAGATTAGTGTTCTCCGTGATGCATGTGCCAAGTTCTTGGAGGAG CAACTTGATCCTTGTAATTGCTTAGGAATCCAGCGCTTTGCTGATACCCATTCACTCAAAACACTCTTC ACAAAATGCAAAAATTTTGCGTTACAGACTTTTGAGGATGTATCCCAGCACGAAGAATTTCTTGAGCTT GACAAAGATGAACTTATTGATTATATTTGTAGTGATGAACTTGTTATTGGTAAAGAGGAGATGGTTTTT GAAGCCGTCATGCGTTGGGTCTATCGTGCCGTTGATCTGAGAAGACCACTGTTACACGAGCTCCTGACA CATGTGAGACTCCCTCTGTTGCATCCCAACTACTTTGTTCAAACAGTTGAAGTGGACCAATTGATCCAG AATTCTCCTGAGTGTTATCAGTTGTTGCATGAAGCAAGACGGTACCACATACTTGGGAATGAAATGATG TCCCCAAGGACTAGGCCACGCAGGTCCACTGGCTATTCTGAGGTGATAGTTGTCGTTGGAGGATGTGAG CGAGTTGGAGGATTTAATCTTCCATACACTGAGTGCTACGATCCTGTAACAGGAGAATGGAAGTCTTTG GCTAAGCTTCCAGAATTTACCAAATCAGAGTATGCAGTCTGTGCTCTAAGGAATGACATTCTTGTTTCA GGTGGAAGAATCAACAGCCGTGATGTCTGGATTTATAACTCACAGTTAAATATTTGGATCAGAGTTGCC TCTCTCAATAAAGGCAGATGGCGTCACAAAATGGCTGTCCTCCTTGGTAAAGTATATGTTGTCGGTGGC TATGATGGGCAAAACAGACTTAGCAGCGTAGAATGTTATGATTCCTTTTCAAATCGATGGACTGAAGTT GCTCCCCTTAAGGAAGCCGTGAGTTCTCCTGCAGTGACTAGCTGTGTAGGCAAACTGTTTGTGATTGGT GGAGGACCTGATGATAATACTTGTTCTGATAAGGTTCAATCTTATGATCCAGAAACCAATTCTTGGCTA CTTCGTGCAGCTATCCCAATTGCCAAAAGGTGTATAACAGCTGTATCCCTAAACAACCTGATCTATGTT GCCGGTGGACTGACCAAGGCAATATACTGTTACGATCCAGTTGAAGATTACTGGATGCACGTACAGAAT ACATTCAGCCGTCAGGAAAACTGTGGTATGTCTGTGTGTAATGGTAAAATATATATCCTGGGCGGAAGA CGGGAAAATGGAGAAGCCACAGACACTATTCTCTGTTATGATCCTGCAACAAGTATCATCACAGGGGTA GCTGCAATGCCCAGGCCAGTGTCCTATCATGGCTGTGTGACTATTCATAGATACAATGAGAAATGCTTT AAACTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_017644 |
| Insert Size | 1803 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_017644.3 |
| RefSeq Size | 7331 bp |
| RefSeq ORF | 1803 bp |
| Locus ID | 54800 |
| UniProt ID | Q6TFL4 |
| Domains | Kelch |
| MW | 68.4 kDa |
| Gene Summary | The protein encoded by this gene is a ubiquitin ligase substrate receptor and is regulated by autoubiquitination. Variations in the translation initiation codon of this gene have been found, which result in an N-terminally truncated but more stable protein due to loss of the autoubiquitination function. The more stable mutant protein causes an increased ubiquitin and degradation of keratin 14, which leads to skin fragility and the potentially life-threatening disease epidermolysis bullosa. The encoded protein is also involved in the regulation of kainate receptors. [provided by RefSeq, Mar 2017] Transcript Variant: This variant (7), as well as variants 8-15, encodes isoform b. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221144 | KLHL24 (Myc-DDK-tagged)-Human kelch-like 24 (Drosophila) (KLHL24) |
CNY 4464.00 |
|
| RC221144L3 | Lenti-ORF clone of KLHL24 (Myc-DDK-tagged)-Human kelch-like 24 (Drosophila) (KLHL24) |
CNY 6460.00 |
|
| RC221144L4 | Lenti-ORF clone of KLHL24 (mGFP-tagged)-Human kelch-like 24 (Drosophila) (KLHL24) |
CNY 6460.00 |
|
| RG221144 | KLHL24 (tGFP-tagged) - Human kelch-like 24 (Drosophila) (KLHL24) |
CNY 6064.00 |
