MAGED4B (NM_177537) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6 series
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_177537, the custom clone sequence may differ by one or more nucleotides ATGGCTGAGGGAAGCTTCAGCGTGCAATCGGAAAGCTACAGTGTTGAAGACATGGATGAG GGTAGCGACGAAGTCGGGGAGGAAGAGATGGTTGAAGGCAACGACTATGAAGAATTCGGT GCGTTTGGTGGCTATGGCACCCTCACCAGCTTTGACATCCATATCCTCAGAGCCTTCGGA AGCTTGGGTCCAGGCCTTCGCATCTTATCGAATGAGCCCTGGGAACTGGAAAACCCTGTG CTGGCCCAGACCCTGGTGGAGGCATTGCAGCTGGATCCGGAAACACTTGCCAATGAGACG GCCGCCCGTGCTGCCAACGTAGCCCGCGCCGCCGCCTCCAACCGTGCGGCTCGGGCCGCT GCCGCCGCTGCCCGTACCGCCTTCAGTCAGGTGGTCGCTAGCCACCGGGTGGCCACGCCG CAGGTCTCAGGAGAGGATACCCAGCCCACGACCTACGCCGCCGAGGCTCAGGGGCCCACC CCTGAGCCACCCCTTGCTTCTCCGCAGACCTCCCAGATGTTAGTCACCAGTAAGATGGCT GCCCCCGAGGCTCCGGCAACCTCCGCACAGTCCCAGACAGGCTCCCCGGCCCAGGAGGCT GCTACTGAGGGCCCTAGTAGCGCCTGTGCTTTCTCTCAGGCTCCGTGTGCCAGGGAGGTG GACGCCAACCGGCCCAGCACAGCCTTCCTGGGCCAGAATGATGTCTTCGATTTCACTCAG CCGGCAGGTGTCAGTGGCATGGCCTTCCCGCGCCCCAAGAGACCTGCCCCAGCCCAAGAG GCTGCCACAGAGGGCCCCAGTGCTGCCTCTGGTGTGCCCCAGACGGGACCTGGCAGGGAG GTGGCAGCCACCCGGCCCAAGACCACCAAGTCGGGGAAGGCGCTGGCCAAGACTCGGTGG GTGGAGCCTCAGAATGTTGTGGCAGCAGCTGCTGCCAAGGCCAAGATGGCCACGAGCATC CCTGAGCCGGAGGGTGCAGCTGCTGCCACTGCTCAGCACAGTGCTGAGCCCTGGGCCAGG ATGGGAGGCAAGAGGACCAAGAAGTCCAAGCACCTGGATGATGAGTATGAGAGCAGCGAG GAGGAGAGAGAGACTCCCGCGGTCCCACCCACCTGGAGAGCATCACAGCCCTCATTGACG GTGCGGGCTCAGTTGGCCCCTCGGCCCCCGATGGCCCCGAGGTCCCAGATACCCTCAAGG CACGTACTGTGCCTGCCCCCCCGCAACGTGACCCTTCTGCAGGAGAGGGCAAATAAGTTG GTGAAATACCTGATGATTAAGGACTACAAGAAGATCCCCATCAAGCGCGCAGACATGCTG AAGGATGTCATCAGAGAATATGATGAACATTTCCCTGAGATCATTGAACGAGCAACGTAC ACCCTGGAAAAGAAGTTTGGGATCCACCTGAAGGAGATCGACAAGGAAGAACACCTGTAT ATTCTTGTCTGCACACGGGACTCCTCAGCTCGCCTCCTTGGAAAAACCAAGGACACTCCC AGGCTGAGTCTCCTCTTGGTGATTCTGGGCGTCATCTTCATGAATGGCAACCGTGCCAGC GAGGCTGTCCTCTGGGAGGCACTACGCAAGATGGGACTGCGCCCTGGGGTGAGGCACCCA TTCCTCGGCGATCTGAGGAAGCTCATCACAGATGACTTTGTGAAGCAGAAGTACCTGGAA TACAAGAAGATCCCCAACAGCAACCCACCTGAGTATGAATTCCTCTGGGGCCTGCGAGCC CGCCATGAGACCAGCAAGATGAGGGTCCTGAGATTCATCGCCCAGAATCAGAACCGAGAC CCCCGGGAATGGAAGGCTCATTTCTTGGAGGCTGTGGATGATGCTTTCAAGACAATGGAT GTGGATATGGCCGAGGAACATGCCAGGGCCCAGATGAGGGCCCAGATGAATATCGGGGAT GAAGCGCTGATTGGACGGTGGAGCTGGGATGACATACAAGTCGAGCTCCTGACCTGGGAT GAGGACGGAGATTTTGGCGATGCCTGGGCCAGGATCCCCTTTGCTTTCTGGGCCAGATAC CATCAGTACATTCTGAATAGCAACCGTGCCAACAGGAGGGCCACGTGGAGAGCTGGCGTC AGCAGTGGCACCAATGGAGGGGCCAGCACCAGCGTCCTAGATGGCCCCAGCACCAGCTCC ACCATCCGGACCAGAAATGCTGCCAGAGCTGGCGCCAGCTTCTTCTCCTGGATCCAG
Restriction Sites	Please inquire
ACCN	NM_177537
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_177537.1, NP_803881.1
RefSeq Size	2585 bp
RefSeq ORF	2220 bp
Locus ID	81557
UniProt ID	Q96JG8
Gene Summary	This gene is a member of the MAGED gene family. It is expressed only in brain and ovary, and some transcript variants of this gene are specifically expressed in glioma cells. This gene is clustered with other MAGED genes on chromosome Xp11. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, May 2011] Transcript Variant: This variant (3, also known as MAGE-E1b) uses an alternate donor splice site at the penultimate exon compared to variant 1. This results in a frame-shift, early translation termination, and a shorter isoform (2) missing 2 aa from the C-terminus compared to isoform 1.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.