ZRANB3 (NM_032143) Human Untagged Clone
CAT＃: SC315157
ZRANB3 (untagged)-Human zinc finger, RAN-binding domain containing 3 (ZRANB3)
CNY 10450.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | 4933425L19Rik; AH2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC315157 representing NM_032143.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCTAGGGTTCATAACATAAAAAAGTCTCTTACACCTCACATTTCTTGTGTGACAAATGAATCTGAT AATCTGCTGGATTTTTTGCCTGACAGACTAAGAGCAAAGCTACTTCCATTCCAGAAAGATGGCATCATT TTTGCCCTCAAAAGAAATGGCAGGTGTATGGTGGCTGATGAAATGGGTCTAGGAAAGACAATCCAGGCA ATTGGAATTACTTACTTCTATAAAGAGGAATGGCCTCTGTTAATAGTGGTCCCTTCGTCTCTGAGGTAC CCTTGGACAGAAGAAATTGAAAAATGGATCCCAGAGCTAAGTCCAGAAGAAATCAATGTTATTCAGAAT AAAACTGATGTTAGGAGAATGTCGACCAGTAAAGTGACAGTTCTGGGTTATGGTCTCTTAACCGCAGAT GCAAAGACTTTGATAGATGCACTGAATAATCAGAACTTCAAAGTAGTTATAGTGGATGAATCACACTAC ATGAAATCCAGAAATGCAACTCGCAGCAGGATTTTATTGCCAATAGTACAGAAAGCCAGACGAGCCATT CTTCTTACAGGAACACCAGCTTTAGGAAGGCCTGAAGAGCTTTTTATGCAGATTGAAGCTCTCTTTCCA CAAAAATTTGGAAGATGGACCGACTATGCAAAAAGATACTGTAATGCACACATCAGATATTTTGGTAAA AGACCTCAGTGGGATTGTAGAGGGGCATCAAATCTTAATGAACTTCACCAGCTATTAAGTGACATAATG ATTAGAAGATTAAAGACTGAAGTTTTAACCCAGCTACCCCCTAAAGTCAGACAGCGTATTCCATTTGAT CTTCCATCAGCAGCTGCCAAGGAATTGAATACCAGCTTTGAAGAGTGGGAAAAAATAATGAGAACTCCA AATTCAGGTGCCATGGAGACAGTCATGGGGTTGATAACTCGCATGTTTAAACAAACTGCTATTGCCAAG GCAGGTGCTGTAAAGGATTATATTAAGATGATGCTTCAGAATGATTCGCTTAAATTTCTGGTTTTTGCT CACCATTTAAGCATGCTCCAAGCTTGCACAGAAGCAGTCATCGAAAATAAGACTCGTTACATTAGGATA GATGGAAGTGTTTCATCTTCAGAAAGAATACATCTGGTTAATCAGTTTCAAAAGGATCCTGACACTCGC GTGGCTATCCTAAGCATTCAGGCTGCTGGCCAGGGATTAACATTTACTGCAGCAAGTCATGTTGTATTT GCTGAGTTGTACTGGGACCCTGGACATATAAAACAAGCAGAAGACCGAGCTCACAGAATTGGCCAGTGC AGTTCTGTGAATATTCACTACCTTATTGCAAATGGAACCCTAGACACCCTTATGTGGGGAATGTTGAAT CGCAAGGCTCAAGTTACAGGGAGCACACTGAACGGTAGGAAAGAAAAAATTCAGGCTGAGGAAGGTGAT AAGGAAAAATGGGATTTCCTGCAGTTTGCTGAAGCTTGGACTCCAAATGACAGTTCTGAAGAGTTAAGG AAGGAAGCTTTGTTCACTCACTTCGAAAAAGAAAAACAGCATGATATTCGATCATTTTTTGTACCACAA CCTAAAAAAAGACAGTTGATGACCTCCTGTGACGAATCAAAGAGATTCCGGGAGGAAAATACTGTAGTG TCATCAGACCCTACAAAAACAGCTGCAAGAGATATCATCGATTATGAAAGTGATGTTGAACCTGAAACG AAAAGATTGAAATTGGCTGCCTCGGAAGACCACTGCAGTCCGTCGGAAGAGACACCATCCCAGTCCAAG CAAATCCGAACTCCACTCGTGGAAAGTGTACAGGAGGCCAAGGCCCAGTTAACCACCCCAGCCTTTCCT GTAGAGGGCTGGCAATGTAGTCTCTGCACCTATATCAATAATTCAGAGTTACCTTATTGTGAAATGTGT GAGACTCCTCAAGGCAGTGCTGTTATGCAAATAGATAGCCTCAACCATATCCAGGATAAAAACGAGAAG GATGATTCTCAGAAAGACACCTCCAAAAAGGTTCAAACTATCTCAGACTGTGAAAAACAAGCCCTTGCA CAGTCAGAACCTGGCCAGTTGGCTGACAGCAAGGAAGAAACACCAAAAATTGAGAAAGAAGACGGACTT ACATCCCAGCCAGGTAATGAACAGTGGAAGAGTTCAGACACTTTGCCAGTGTATGACACCTTAATGTTC TGTGCAAGTAGGAATACTGACCGGATTCACATCTATACTAAGGATGGAAAACAGATGAGCTGTAATTTC ATTCCTCTGGATATAAAATTAGACCTTTGGGAAGATTTACCAGCAAGCTTTCAGCTGAAACAATATCGC TCACTGATTTTGAGATTTGTTCGAGAATGGAGTAGTCTAACTGCCATGAAGCAAAGGATAATCAGGAAA AGTGGACAGCTATTCTGTAGCCCAATTCTTGCTTTGGAAGAGATCACAAAGCAACAAACCAAACAAAAT TGCACCAAAAGATACATAACCAAAGAAGATGTTGCCGTAGCCTCAATGGACAAAGTGAAGAATGTTGGG GGCCATGTCCGTCTGATCACAAAGGAGTCCAGGCCACGGGATCCTTTCACAAAAAAACTTCTTGAAGAT GGAGCCTGTGTCCCATTTCTAAATCCATACACAGTCCAGGCAGATCTCACTGTGAAGCCCTCTACATCC AAAGGCTATTTGCAAGCTGTGGATAATGAAGGAAATCCACTTTGCCTTCGCTGTCAGCAACCCACTTGC CAGACTAAGCAAGCATGTAAAGCGAACTCTTGGGATTCACGGTTTTGCTCTCTGAAATGTCAGGAAGAG TTTTGGATTCGATCTAATAACAGTTACCTGAGAGCCAAAGTATTTGAAACTGAACATGGTGTGTGTCAG CTCTGTAATGTGAACGCACAAGAACTCTTTTTACGTCTGAGAGATGCCCCTAAAAGTCAGAGGAAGAAT CTTCTGTATGCTACCTGGACTTCAAAGCTCCCATTAGAACAGCTAAATGAAATGATAAGAAACCCAGGG GAAGGACATTTCTGGCAGGTGGATCACATCAAGCCAGTGTATGGGGGAGGAGGACAGTGTTCCCTGGAC AACCTGCAGACTCTCTGCACAGTCTGTCACAAAGAGAGAACTGCCAGACAAGCTAAGGAAAGAAGCCAG GTGAGAAGACAATCTCTAGCATCAAAGCATGGATCAGACATCACACGATTTTTGGTAAAGAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_032143 |
| Insert Size | 3240 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032143.3 |
| RefSeq Size | 6740 bp |
| RefSeq ORF | 3240 bp |
| Locus ID | 84083 |
| UniProt ID | Q5FWF4 |
| Domains | zf-RanBP, HNH, HNHc |
| Protein Families | Druggable Genome |
| MW | 123.2 kDa |
| Gene Summary | DNA annealing helicase and endonuclease required to maintain genome stability at stalled or collapsed replication forks by facilitating fork restart and limiting inappropriate recombination that could occur during template switching events (PubMed:21078962, PubMed:22704558, PubMed:22705370, PubMed:22759634, PubMed:26884333). Recruited to the sites of stalled DNA replication by polyubiquitinated PCNA and acts as a structure-specific endonuclease that cleaves the replication fork D-loop intermediate, generating an accessible 3'-OH group in the template of the leading strand, which is amenable to extension by DNA polymerase (PubMed:22759634). In addition to endonuclease activity, also catalyzes the fork regression via annealing helicase activity in order to prevent disintegration of the replication fork and the formation of double-strand breaks (PubMed:22705370, PubMed:22704558).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222784 | ZRANB3 (Myc-DDK-tagged)-Human zinc finger, RAN-binding domain containing 3 (ZRANB3) |
CNY 7512.00 |
|
| RC222784L1 | Lenti ORF clone of Human zinc finger, RAN-binding domain containing 3 (ZRANB3), Myc-DDK-tagged |
CNY 9912.00 |
|
| RC222784L2 | Lenti ORF clone of Human zinc finger, RAN-binding domain containing 3 (ZRANB3), mGFP tagged |
CNY 10260.00 |
|
| RC222784L3 | Lenti ORF clone of Human zinc finger, RAN-binding domain containing 3 (ZRANB3), Myc-DDK-tagged |
CNY 10260.00 |
|
| RC222784L4 | Lenti ORF clone of Human zinc finger, RAN-binding domain containing 3 (ZRANB3), mGFP tagged |
CNY 10260.00 |
|
| RG222784 | ZRANB3 (tGFP-tagged) - Human zinc finger, RAN-binding domain containing 3 (ZRANB3) |
CNY 9220.00 |
