CGNL1 (NM_032866) Human Untagged Clone
CAT＃: SC315346
CGNL1 (untagged)-Human cingulin-like 1 (CGNL1)
CNY 13608.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | JACOP; PCING |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC315346 representing NM_032866.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGCTGTATTTCGGTGAATATCAACATGTGCAGCAGGAATATGGGGTCCATCTGAGACTCGCAAGT GATGATACCCAAAAATCAAGGAGTTCCCAGAACTCCAAGGCAGGCTCCTACGGTGTCAGTATTCGGGTC CAGGGAATTGATGGTCACCCCTATATTGTCCTGAATAACACAGAACGGTGCCTAGCAGGCACATCGTTT TCTGAAAATGGGCCACCCTTTCCACCTCCAGTGATAAATAACCTGCCTCTACATTCCAGCAATGGTTCT GTGCCAAAGGAGAACAGTGAAGAACTTCAGCTTCCAGAAAACCCATACGCCCAGCCTAGCCCAATAAGA AACCTGAAACAGCCCCTGCTCCATGAGGGCAAGAATGGAGTTCTAGATCGCAAAGACGGGTCTGTGAAG CCATCTCACCTGCTGAACTTTCAGAGGCATCCAGAGCTTTTGCAACCCTATGACCCTGAAAAGAATGAG TTGAATTTACAAAATCACCAGCCTTCTGAGAGTAATTGGCTAAAAACGTTGACAGAAGAAGGCATCAAC AATAAGAAGCCTTGGACTTGCTTTCCCAAACCTAGCAATTCCCAGCCTACCAGTCCCTCCTTGGAAGAC CCGGCCAAATCTGGTGTGACAGCTATTCGTTTATGCAGCTCCGTGGTCATAGAGGACCCCAAAAAGCAG ACCTCAGTGTGTGTAAACGTTCAGAGCTGCACCAAGGAGAGGGTGGGAGAGGAGGCCCTTTTCACTAGC GGGAGGCCCCTGACTGCCCACAGCCCACATGCCCACCCTGAAACCAAGAAAACCAGGCCAGATGTTCTT CCCTTCCGGCGACAGGATTCAGCGGGACCCGTCCTGGATGGAGCTCGGTCCCGGAGGTCCTCCTCGTCA TCCACAACTCCCACGTCAGCCAACTCTTTGTACAGGTTTTTACTGGATGATCAGGAATGTGCCATCCAT GCCGACAACGTCAATCGTCATGAAAACAGAAGGTATATTCCCTTCCTGCCAGGAACTGGACGGGATATT GATACAGGATCAATTCCTGGTGTGGATCAGTTAATTGAAAAATTTGATCAAAAACCTGGGCTTCAGAGA AGAGGAAGGTCTGGGAAGCGAAACAGAATTAATACAGATGACAGGAAAAGATCCAGAAGCGTGGATAGC GCCTTTCCTTTTGGCCTCCAAGGGAACTCGGAGTACCTGATTGAATTCAGTAGGAACTTGGGCAAGTCA AGCGAACACCTCCTCCGGCCTTCCCAGGTGTGCCCGCAGCGGCCACTGTCTCAGGAGCGCCGTGGGAAA CAGAGCGTGGGCCGCACCTTTGCAAAGCTGCAGGGAGCAGCGCACGGGGCTTCATGTGCCCACTCCAGG CCTCCCCAGCCGAACATAGATGGGAAAGTTCTGGAAACCGAAGGTAGTCAGGAAAGTACAGTGATCCGT GCGCCCTCCCTTGGTGCACAGAGTAAAAAGGAGGAGGAGGTGAAAACAGCCACCGCTACGCTGATGTTA CAGAACCGGGCAACAGCAACTTCGCCTGATTCTGGTGCCAAGAAAATTTCCGTGAAGACATTTCCTTCG GCCTCAAATACTCAGGCCACACCGGATCTCTTAAAGGGCCAGCAAGAGCTCACTCAGCAAACCAATGAG GAGACAGCTAAGCAGATTCTCTACAATTACCTCAAAGAAGGAAGCACTGATAATGACGATGCTACTAAA AGGAAAGTCAACTTGGTCTTTGAGAAAATCCAGACCTTAAAGTCTCGAGCAGCTGGGAGCGCCCAAGGA AATAACCAAGCTTGTAATTCCACATCTGAAGTCAAAGATCTATTGGAACAGAAAAGCAAGTTGACCATA GAAGTGGCTGAACTTCAGAGACAGCTTCAACTGGAAGTCAAGAATCAACAGAACATTAAAGAAGAGAGA GAGAGGATGAGAGCAAACCTAGAAGAGCTCCGAAGCCAACACAACGAAAAGGTGGAGGAGAACTCCACA TTGCAGCAACGACTGGAAGAAAGTGAAGGGGAGCTCCGGAAGAATCTGGAGGAGCTATTCCAGGTGAAG ATGGAACGGGAGCAGCATCAGACTGAGATCAGGGATCTCCAGGACCAGCTCTCAGAAATGCACGATGAA CTGGACAGTGCAAAGCGATCGGAGGACAGGGAGAAGGGAGCTCTGATTGAGGAGCTCTTACAGGCAAAA CAGGATCTTCAAGATCTGCTGATTGCCAAAGAGGAGCAAGAAGACCTCTTGAGAAAGCGAGAGCGTGAA CTCACCGCCCTGAAGGGAGCCCTGAAAGAAGAGGTTTCCAGCCATGATCAGGAGATGGACAAGCTGAAG GAGCAATATGATGCTGAGTTGCAGGCCCTGAGGGAGAGTGTGGAAGAAGCAACCAAGAATGTCGAGGTC TTGGCGAGCAGGAGCAACACTTCAGAGCAAGACCAGGCGGGGACTGAAATGCGCGTGAAGCTTCTGCAG GAGGAGAATGAGAAGCTGCAGGGAAGAAGCGAAGAGCTGGAGCGGAGAGTTGCTCAGCTTCAAAGGCAG ATCGAGGACCTGAAAGGCGATGAAGCCAAGGCGAAGGAAACGCTGAAGAAGTACGAGGGAGAAATACGA CAGTTAGAGGAGGCCCTTGTGCACGCCAGAAAGGAAGAAAAAGAAGCTGTGTCAGCCAGAAGGGCCCTG GAGAATGAACTGGAGGCTGCTCAGGGAAATCTGAGTCAGACTACCCAGGAGCAGAAGCAGTTGTCTGAG AAGCTCAAAGAGGAGAGTGAGCAGAAGGAGCAGCTAAGAAGGTTGAAGAACGAGATGGAGAATGAGCGG TGGCACCTGGGCAAAACCATTGAGAAACTGCAGAAGGAGATGGCAGACATTGTTGAGGCCTCCCGTACC TCAACCCTGGAGCTCCAGAACCAGCTGGATGAGTATAAGGAGAAAAACCGCAGGGAGCTCGCAGAAATG CAAAGACAGTTGAAGGAGAAAACGCTGGAGGCAGAAAAGTCCCGACTGACAGCCATGAAAATGCAGGAT GAGATGCGTCTGATGGAGGAAGAGTTACGGGACTACCAGAGAGCTCAGGATGAAGCACTCACAAAAAGG CAGCTTCTGGAGCAGACGCTGAAGGACCTGGAGTATGAGCTGGAAGCCAAGAGTCACCTCAAAGATGAC CGCAGCAGGCTGGTCAAGCAGATGGAGGACAAGGTGTCTCAACTGGAGATGGAACTGGAAGAAGAGAGA AACAACTCAGATTTGCTGTCTGAGAGGATCAGTAGGAGCAGGGAACAGATGGAGCAGTTGAGGAATGAG CTACTTCAGGAGAGAGCTGCGAGACAAGACTTGGAGTGCGACAAGATTTCCCTGGAGAGGCAGAACAAG GACTTAAAGAGCCGGATTATCCACCTGGAAGGTTCCTACAGGTCCAGCAAAGAGGGGCTGGTTGTGCAG ATGGAGGCCAGGATCGCGGAGCTGGAGGACCGCCTGGAGAGTGAGGAGAGGGATCGGGCCAATCTTCAG CTCAGCAACCGGCGGCTGGAGCGGAAAGTGAAGGAGCTGGTGATGCAGGTGGATGATGAGCACCTGTCA TTGACTGATCAGAAGGACCAGCTGAGCTTGCGTTTGAAAGCCATGAAGCGGCAGGTGGAGGAGGCTGAG GAGGAAATCGACAGACTGGAAAGTTCTAAAAAGAAGCTGCAGAGGGAGCTGGAGGAGCAGATGGACATG AATGAGCATCTGCAGGGGCAGCTCAACTCCATGAAGAAGGACTTAAGACTGAAGAAGCTGCCGAGTAAA GTGCTGGATGACATGGATGACGACGATGACCTCAGCACGGATGGGGGAAGCCTCTATGAGGCGCCTGTG AGCTACACATTCTCCAAGGACAGCACCGTCGCCAGCCAGATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_032866 |
| Insert Size | 3909 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032866.3 |
| RefSeq Size | 7235 bp |
| RefSeq ORF | 3909 bp |
| Locus ID | 84952 |
| UniProt ID | Q0VF96 |
| MW | 149.1 kDa |
| Gene Summary | This gene encodes a member of the cingulin family. The encoded protein localizes to both adherens and tight cell-cell junctions and mediates junction assembly and maintenance by regulating the activity of the small GTPases RhoA and Rac1. Heterozygous chromosomal rearrangements resulting in association of the promoter for this gene with the aromatase gene are a cause of aromatase excess syndrome. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC223121 | CGNL1 (Myc-DDK-tagged)-Human cingulin-like 1 (CGNL1) |
CNY 13600.00 |
|
| RC223121L3 | Lenti ORF clone of Human cingulin-like 1 (CGNL1), Myc-DDK-tagged |
CNY 7600.00 |
|
| RC223121L4 | Lenti ORF clone of Human cingulin-like 1 (CGNL1), mGFP tagged |
CNY 7600.00 |
|
| RG223121 | CGNL1 (tGFP-tagged) - Human cingulin-like 1 (CGNL1) |
CNY 15200.00 |
