SEC31A (NM_001077208) Human Untagged Clone
CAT＃: SC315648
SEC31A (untagged)-Human SEC31 homolog A (S. cerevisiae) (SEC31A), transcript variant 3
CNY 10450.00
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热销推荐Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ABP125; ABP130; HSPC275; HSPC334; NEDSOSB; SEC31L1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC315648 representing NM_001077208.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAGTTAAAGGAAGTAGATCGTACAGCCATGCAGGCATGGAGCCCTGCCCAGAATCACCCCATTTAC CTAGCAACAGGAACATCTGCTCAGCAATTGGATGCAACATTTAGTACGAATGCTTCCCTTGAGATATTT GAATTAGACCTCTCTGATCCATCCTTGGATATGAAATCTTGTGCCACATTCTCCTCTTCTCACAGGTAC CACAAGTTGATTTGGGGGCCTTATAAAATGGATTCCAAAGGAGATGTCTCTGGAGTTCTGATTGCAGGT GGTGAAAATGGAAATATTATTCTCTATGATCCTTCTAAAATTATAGCTGGAGACAAGGAAGTTGTGATT GCCCAGAATGACAAGCATACTGGCCCAGTGAGAGCCTTGGATGTGAACATTTTCCAGACTAATCTGGTA GCTTCTGGTGCTAATGAATCTGAAATCTACATATGGGATCTAAATAATTTTGCAACCCCAATGACACCA GGAGCCAAAACACAGCCGCCAGAAGATATCAGCTGCATTGCATGGAACAGACAAGTTCAGCATATTTTA GCATCAGCCAGTCCCAGTGGCCGGGCCACTGTATGGGATCTTAGAAAAAATGAGCCAATCATCAAAGTC AGTGACCATAGTAACAGAATGCATTGTTCTGGGTTGGCATGGCATCCTGATGTTGCTACTCAGATGGTC CTTGCCTCCGAGGATGACCGGTTACCAGTGATCCAGATGTGGGATCTTCGATTTGCTTCCTCTCCACTT CGTGTCCTGGAAAACCATGCCAGGGGGATTTTGGCAATTGCTTGGAGCATGGCAGATCCTGAATTGTTA CTGAGCTGTGGAAAAGATGCTAAGATTCTCTGCTCCAATCCAAACACAGGAGAGGTGTTATATGAACTT CCCACCAACACACAGTGGTGCTTCGATATTCAGTGGTGTCCCCGAAATCCTGCTGTCTTATCAGCTGCT TCGTTTGATGGGCGTATCAGTGTTTATTCTATCATGGGAGGAAGCACAGATGGTTTAAGACAGAAACAA GTTGACAAGCTTTCATCATCTTTTGGGAATCTTGATCCCTTTGGCACAGGACAGCCCCTTCCTCCGTTA CAAATTCCACAGCAGACTGCTCAGCATAGTATAGTGCTGCCTCTGAAGAAGCCGCCCAAGTGGATTCGA AGGCCTGTTGGTGCTTCTTTTTCATTTGGAGGCAAACTGGTTACGTTTGAGAATGTCAGAATGCCTTCT CATCAGGGAGCTGAGCAGCAGCAGCAGCAGCACCATGTGTTCATTAGTCAGGTTGTAACAGAAAAGGAG TTCCTCAGCCGATCAGACCAACTTCAGCAGGCTGTGCAGTCACAAGGATTTATCAATTATTGCCAAAAA AAAATTGATGCTTCTCAGACTGAATTTGAGAAAAATGTGTGGTCCTTTTTGAAGGTAAACTTTGAGGAT GATTCTCGTGGAAAATACCTTGAACTTCTAGGATACAGAAAAGAAGATCTAGGAAAGAAGATTGCTTTG GCCTTGAACAAAGTGGATGGAGCCAATGTGGCTCTTAAAGACTCTGACCAAGTAGCACAGAGTGATGGG GAGGAGAGCCCTGCTGCTGAAGAGCAGCTCTTGGGAGAGCACATTAAAGAGGAAAAAGAAGAATCTGAA TTTCTACCCTCATCTGGAGGAACATTTAATATCTCTGTCAGTGGGGACATTGATGGTTTAATTACTCAG GCTTTGCTGACGGGCAATTTTGAGAGTGCTGTTGACCTTTGTTTACATGATAACCGCATGGCCGATGCC ATTATATTGGCCATAGCAGGTGGACAAGAACTCTTGGCTCGAACCCAGAAAAAATACTTCGCAAAATCC CAAAGCAAAATTACCAGGCTCATCACTGCAGTGGTGATGAAGAACTGGAAAGAGATTGTTGAGTCTTGT GATCTTAAAAATTGGAGAGAGGCTTTAGCTGCAGTATTGACTTATGCAAAGCCGGATGAATTTTCAGCC CTTTGTGATCTTTTGGGAACCAGGCTTGAAAATGAAGGAGATAGCCTCCTGCAGACTCAAGCATGTCTC TGCTATATTTGTGCAGGGAATGTAGAGAAATTAGTTGCATGTTGGACTAAAGCTCAAGATGGAAGCCAC CCTTTGTCACTTCAGGATCTGATTGAGAAAGTTGTCATCCTGCGAAAAGCTGTGCAACTCACTCAAGCC ATGGACACTAGTACTGTAGGAGTTCTCTTGGCTGCGAAGATGAGTCAGTATGCCAATTTGTTGGCAGCT CAGGGCAGTATTGCTGCAGCCTTGGCTTTTCTTCCTGACAACACCAACCAGCCAAATATCATGCAGCTT CGTGACAGACTTTGTAGAGCACAAGGAGAGCCTGTAGCAGGACATGAATCACCTAAAATTCCGTACGAG AAACAGCAGCTCCCCAAGGGCAGGCCTGGACCAGTTGCTGGCCACCACCAGATGCCAAGAGTTCAAACT CAACAATATTATCCCCATGGAGAAAATCCTCCACCTCCGGGTTTCATAATGCATGGAAATGTTAATCCA AATGCTGCTGGTCAGCTTCCCACATCTCCAGGTCATATGCACACCCAGGTACCACCTTATCCACAGCCA CAGCCTTATCAACCAGCCCAGCCGTATCCCTTCGGAACAGGGGGGTCAGCAATGTATCGACCTCAGCAG CCTGTTGCTCCTCCTACTTCAAACGCTTACCCTAACACCCCTTACATATCTTCTGCTTCTTCCTATACT GGGCAGTCTCAGCTGTACGCAGCACAGCACCAGGCCTCTTCACCTACCTCCAGCCCTGCTACTTCTTTC CCTCCTCCCCCTTCCTCTGGAGCATCCTTCCAGCATGGCGGACCAGGAGCTCCACCATCATCTTCAGCT TATGCACTGCCTCCTGGAACAACAGGTCCTCAGAATGGTTGGAATGACCCTCCAGCTTTGAACAGAGTA CCCAAAAAGAAGAAGATGCCTGAAAACTTCATGCCTCCTGTTCCCATCACATCACCAATCATGAACCCG TTGGGTGACCCCCAGTCACAAATGCTGCAGCAACAGCCTTCAGCTCCAGTACCACTGTCAAGCCAGTCT TCATTCCCACAGCCACATCTTCCAGGTGGCCAGCCCTTCCATGGCGTACAGCAACCTCTTGGTCAAACA GGCATGCCACCATCTTTTTCAAAGCCCAATATTGAAGGTGCCCCAGGGGCTCCTATTGGAAATACCTTC CAGCATGTGCAGTCTTTGCCAACAAAAAAAATTACCAAGAAACCTATTCCAGATGAGCACCTCATTCTA AAGACCACATTTGAGGATCTTATTCAGCGCTGCCTTTCTTCAGCAACAGACCCTCAAACCAAGAGGAAG CTAGATGATGCCAGCAAACGTTTGGAGTTTCTGTATGATAAACTTAGGGAACAGACACTTTCACCAACA ATCACCAGTGGTTTACACAACATTGCAAGGAGCATTGAAACTCGAAACTACTCAGAAGGATTGACCATG CATACCCACATAGTTAGCACCAGCAACTTCAGTGAGACCTCTGCTTTCATGCCAGTTCTCAAAGTTGTT CTCACCCAGGCCAATAAGCTGGGTGTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001077208 |
| Insert Size | 3618 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001077208.3 |
| RefSeq Size | 4411 bp |
| RefSeq ORF | 3618 bp |
| Locus ID | 22872 |
| UniProt ID | O94979 |
| MW | 131.5 kDa |
| Gene Summary | The protein encoded by this gene shares similarity with the yeast Sec31 protein, and is a component of the outer layer of the coat protein complex II (COPII). The encoded protein is involved in vesicle budding from the endoplasmic reticulum (ER) and contains multiple WD repeats near the N-terminus and a proline-rich region in the C-terminal half. It associates with the protein encoded by the SEC13 homolog, nuclear pore and COPII coat complex component (SEC13), and is required for ER-Golgi transport. Monoubiquitylation of this protein by CUL3-KLHL12 was found to regulate the size of COPII coats to accommodate unusually shaped cargo. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015] Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 5. The encoded isoform (3) has the same N- and C-termini, but is shorter than isoform 1. Both variants 3 and 9 encode the same isoform (3). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC216802 | SEC31A (Myc-DDK-tagged)-Human SEC31 homolog A (S. cerevisiae) (SEC31A), transcript variant 3 |
CNY 12584.00 |
|
| RC216802L3 | Lenti ORF clone of Human SEC31 homolog A (S. cerevisiae) (SEC31A), transcript variant 3, Myc-DDK-tagged |
CNY 13970.00 |
|
| RC216802L4 | Lenti ORF clone of Human SEC31 homolog A (S. cerevisiae) (SEC31A), transcript variant 3, mGFP tagged |
CNY 13970.00 |
|
| RG216802 | SEC31A (tGFP-tagged) - Human SEC31 homolog A (S. cerevisiae) (SEC31A), transcript variant 3 |
CNY 14184.00 |
