ECM29 (KIAA0368) (NM_001080398) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ECM29
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC315900 representing NM_001080398. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGACCTCACGGACAAAGCGAAGGCAGAAAGTTAAAATCAGCATATCGAAAATAAATCACTCTAGTTAC CGAAAAGAACATAGGCCGTTTTGGGAGCACCAGCCACCTCCACACCAGAACTGGGCTGTCGGGGGCCGC TGCGGCGCAGGGGCTCGTCCGGGACTGAGTCAGCGCCGGGGCCGAGAAGTTCGGCGGGAGGCGCAGGGC GGGGCTTCCTCCCGCCGACTCCGAGCCCGGCTCTACTTTTCTGAGTCCACGTCTCTTCCTTTGGACAGG CGTGGCAGCCCGGGCCCGCTCGGCCCAGCCCCTCACGGACTGCGGCGCGCGGCTCCCCGGGGCCCGGCC CGCCGAACGCCTCGCTCTACAGCCCTCACCTCGCGGCGCCGAGCGCAGCGTCCGCGGCATGGAGACCGG CTCCGACTCAGGTCAGAGGCCCGCCCCCGGGCCGCGGGGGACTGGCCCTGCGCGCGCGGCGGCGCGGTT AGGCCGGGCCGCCCGCACAGGCCGCCGCGTGTCGAGTCCGCGCGGGGAGCGCACGCGGGGCGGGCGGCG ACAGATCAGCTTGAACGGGTCTTTTTACGACTTGGCCATGCTGAAACAGATGAACAATTACAGAATATT ATATCTAAATTCCTTCCTCCTGTTTTGCTCAAACTCTCTAGCACCCAAGAAGGAGTACGTAAAAAGGTA ATGGAACTGCTGGTCCATCTGAATAAACGTATAAAAAGCCGCCCCAAAATACAACTTCCAGTAGAGACA CTGTTGGTTCAGTACCAGGACCCTGCTGCAGTTTCCTTTGTCACAAATTTTACTATAATTTATGTTAAA ATGGGCTATCCTCGCCTACCAGTGGAAAAACAATGTGAACTGGCCCCTACGCTTCTTACTGCCATGGAA GGGAAGCCTCAGCCACAGCAGGATAGCTTAATGCATCTTTTAATACCAACCCTTTTTCACATGAAATAC CCTGTTGAATCATCAAAATCAGCTTCTCCATTTAATCTTGCTGAGAAACCAAAGACTGTGCAGCTGCTT TTGGACTTCATGCTAGATGTCCTTCTGATGCCTTATGGTTACGTGTTAAATGAATCCCAGAGTCGCCAA AATTCATCTTCAGCACAGGGTTCTTCTTCAAACAGTGGCGGAGGTTCTGGAATCCCACAGCCTCCTCCG GGAATGAGCTTTTATGCAGCCAAACGAGTTATTGGTGATAACCCATGGACACCTGAACAATTGGAACAG TGCAAATTGGGAATCGTGAAATTCATAGAAGCTGAACAGGTGCCTGAACTTGAAGCTGTTCTCCACTTG GTGATTGCCTCTAGTGATACACGCCACAGTGTGGCAACGGCAGCAGACCTGGAATTGAAAAGCAAACAG AGCTTAATTGACTGGAATAATCCTGCCATCATTAATAAGATGTACAAGGTGTACCTTGGAGATATACCA CTGAAGACAAAAGAGGGTGCAGTTCTGAAGCCAGAGTTGAAAAGGGACCCTGTCAGTACAAGAGTCAAG TTAAAGATTGTCCCCCATCTCCTCCGCTCTAGACAAGCTGCTGAAACGTTCCCAGCCAACATTCAGGTG GTGTATGATGGACTTTTTGGTACAAATACAAATTCAAAGTTAAGAACATTATCCCTGCAATTTGTGCAT CATATTTGTATAACCTGTCCAGAAATCAAGATTAAGCCATTAGGTCCAATGCTTTTGAATGGCCTCACC AAGCTAATCAATGAATACAAAGAGGACCCTAAACTACTGTCAATGGCATATTCAGCTGTTGGAAAACTC TCCAGTCGGATGCCACATTTATTCACTAAGGATATAGCGCTTGTGCAGCAGCTTTTTGAAGCCCTTTGC AAGGAAGAGCCTGAGACTCGACTTGCTATTCAAGAAGCTTTATCTATGATGGTTGGAGCGTATAGTACT TTGGAAGGGGCACAGCGAACTCTCATGGAGGCACTTGTGGCTTCGTACTTAATAAAGCCTGAAGTTCAA GTTCGACAAGTGGCTGTGAAATTTGCCAGTACGGTGTTTCCCTCAGATCATATCCCTTCCAGATATTTG CTGCTACTGGCTGCAGGAGATCCACGTGAAGAAGTTCATGGAGAAGCACAACGCGTATTAAGGTGTCTT CCAGGTAGAAACAGAAAAGAAAGTACTTCTGAGCAGATGCCTTCCTTCCCAGAAATGGTTTATTACATC CAAGAAAAGGCTTCTCATCGAATGAAAACTCCAGTCAAGTACATGACCGGGACCACTGTCCTTCCATTT AACCCAGCAGCCTTTGGAGAGATCGTTCTGTACTTGCGCATGTGCCTTGCGCACAGTGCGGGGGTGGTG CCCACCTCTCAGAGTTTGGCTGATATGCAGGATCATGCCCCAGCCATTGGGCGCTACATACGGACTTTA ATGTCAAGCGGGCAGATGGCACCCTCATCATCTAACAAGAGTGGGGAGACTAACCCTGTCCAGATCTAC ATTGGCCTGCTTCAGCAGCTGTTAGCAGGTGTTGGAGGTTTGCCGGTTATGTACTGTCTATTGGAAGCT GTGTCAGTGTATCCAGAAAAGCTGGCTACCAAATTTGTAGACAAAACAGAATGGATAAAGAGTCTGATG AATAACAGTAAAGAAGAAATGCGCGAACTGGCAGCGTTGTTTTATTCTGTAGTGGTATCAACAGTGTCG GGGAATGAGTTGAAATCAATGATAGAACAGCTTATAAAGACTACAAAAGACAATCACAGCCCGGAGATA CAGCATGGATCCTTGCTTGCATTGGGATTCACGGTGGGAAGGTATTTGGCTAAAAAGAAAATGAGAATG TCAGAGCAACAAGACCTGGAGAGAAATGCTGACACCCTCCCTGATCAAGAGGAACTCATTCAGAGTGCT ACAGAAACAATAGGCTCATTTTTGGACAGTACATCACCCCTCCTGGCAATTGCTGCCTGCACAGCCCTG GGTGAAATTGGCAGAAATGGTCCACTTCCAATCCCCAGTGAGGGATCTGGCTTTACCAAATTGCATCTT GTAGAAAGCTTACTAAGTAGAATACCTTCCAGTAAAGAAACAAATAAGATGAAAGAACGAGCAATCCAA ACACTGGGATATTTTCCAGTTGGGGATGGAGATTTTCCTCACCAGAAACTCCTCTTGCAAGGTCTGATG GATTCTGTGGAGGCCAAGCAGATAGAACTTCAGTTCACTATTGGCGAAGCCATTACCAGTGCTGCAATA GGAACTAGTTCTGTGGCTGCCCGAGATGCCTGGCAAATGACTGAAGAGGAATATACTCCACCTGCTGGA GCCAAAGTGAATGATGTGGTTCCATGGGTGTTGGATGTGATTTTAAATAAACATATCATCAGCCCCAAC CCACACGTGAGGCAAGCAGCCTGCATCTGGCTCCTTTCCCTTGTCAGGAAGCTAAGTACCCACAAAGAA GTGAAGTCTCATCTTAAAGAAATTCAAAGTGCATTTGTTTCAGTTCTATCAGAAAATGATGAACTTAGC CAAGATGTTGCATCAAAGGGCCTTGGGTTGGTTTATGAACTAGGCAATGAACAAGATCAACAGGAATTG GTTTCTACACTTGTGGAAACACTTATGACTGGCAAAAGAGTTAAACATGAAGTTTCTGGAGAGACAGTG GTATTTCAAGGGGGAGCTCTTGGCAAAACACCAGATGGTCAGGGCCTTTCTACTTACAAGGAACTTTGT TCTCTGGCAAGTGATCTTAGCCAGCCAGATCTGGTGTATAAATTTATGAATTTAGCCAACCATCATGCA ATGTGGAACTCTAGGAAGGGTGCTGCTTTTGGTTTTAATGTAATTGCTACCAGAGCTGGAGAGCAGCTG GCTCCTTTTCTGCCTCAGCTAGTTCCTCGACTTTATCGTTACCAGTTTGATCCCAACCTTGGCATTCGA CAGGCCATGACAAGTATTTGGAATGCGTTGGTCACTGACAAATCCATGGTGGATAAATATTTGAAAGAA ATTCTTCAAGATTTGGTTAAGAACCTTACAAGCAATATGTGGCGAGTTCGAGAATCCAGCTGTTTAGCT TTGAATGATTTATTGAGAGGAAGACCCTTAGATGACATCATTGATAAACTTCCAGAAATTTGGGAAACG CTTTTTAGAGTACAAGATGATATCAAGGAATCTGTACGAAAAGCGGCAGAACTAGCTCTGAAAACTCTG AGCAAGGTCTGTGTGAAAATGTGTGACCCTGCCAAAGGAGCAGCTGGCCAGAGAACCATCGCTGCCCTT CTGCCTTGCCTTCTGGACAAAGGAATGATGAGCACCGTGACGGAAGTTCGAGCCCTCAGCATTAACACC CTTGTGAAGATCAGCAAAAGTGCAGGAGCCATGTTGAAACCGCATGCACCAAAACTCATTCCAGCTCTG CTAGAGTCCTTAAGTGTATTGGAGCCCCAAGTTCTCAATTATTTGAGCCTCCGGGCGACAGAGCAAGAA AAGGCTGCGATGGATAGTGCTCGGCTTAGTGCTGCCAAATCTTCTCCAATGATGGAAACAATCAACATG TGCCTGCAATACCTTGATGTGTCAGTGCTGGGCGAGCTAGTTCCTAGGTTGTGTGAACTGATCAGAAGT GGTGTAGGTCTTGGAACTAAGGGTGGCTGTGCCAGTGTCATTGTGTCATTAACTACTCAGTGTCCTCAG GACCTAACACCTTACTCAGGTAAACTTATGAGTGCTTTGCTGAGTGGCCTGACAGATCGGAACAGTGTG ATTCAGAAATCTTGTGCATTTGCTATGGGCCATTTAGTTCGGACCTCACGGGATAGCAGCACTGAAAAA CTCCTGCAGAAGCTCAATGGGTGGTATATGGAGAAAGAAGAACCTATCTACAAGACCTCTTGTGCTTTG ACTATTCATGCTATTGGACGATACAGCCCTGATGTATTAAAGAATCATGCAAAAGAAGTCCTGCCTCTG GCATTTTTAGGCATGCATGAAATTGCTGATGAGGAGAAATCCGAAAAAGAAGAATGTAATTTATGGACC GAAGTGTGGCAGGAAAACGTACCTGGATCCTTTGGTGGCATTCGATTATACCTGCAGGAGTTAATTACT ATTACCCAGAAGGCTTTGCAGTCTCAGTCCTGGAAAATGAAAGCCCAGGGTGCAATTGCCATGGCATCA ATTGCAAAACAGACTAGCTCTCTAGTACCTCCATATCTCGGAATGATACTGACCGCATTGCTGCAAGGC CTGGCTGGAAGAACGTGGGCAGGAAAGGAGGAGCTATTGAAAGCCATTGCCTGTGTGGTGACAGCTTGC AGTGCAGAGCTGGAAAAGTCTGTGCCCAATCAACCCAGCACAAATGAAATTCTTCAAGCTGTTCTGAAG GAATGTAGCAAAGAGAATGTCAAATACAAGATTGTAGCAATCAGCTGTGCAGCTGATATCTTGAAGGCC ACCAAAGAGGACAGATTCCAGGAGTTCTCTAACATTGTCATACCTCTCATCAAGAAGAACTCACTTGAA AGCAGTGGGGTCCGGACAACCAAAAATGAAGAGGAGAATGAAAAGGAAAAGGAGCTCCAGCTGGAATAT CTGCTGGGTGCCTTTGAAAGCCTGGGCAAAGCCTGGCCGCGAAACGCGGAGACCCAACGTTGTTATCGT CAGGAGCTGTGCAAACTGATGTGTGAACGGCTAAAACTCAGCACGTGGAAAGTGCAGCTAGGAGTCCTG CAATCAATGAATGCCTTTTTTCAGGGGTTAATGCTTTTGGAAGAAGAACATGCCGATCCTGAGGCTTTG GCTGAAATTCTGCTTGAAACTTGTAAATCAATCACATATTCTTTAGAAAATAAGACCTACTCATCTGTG AGAACAGAAGCTTTATCTGTGATAGAATTGCTGCTTAAAAAACTTGAAGAATCTAAACAGTGGGAATGT TTGACATCTGAATGCAGAGTGCTCCTAATTGAGTCTTTAGCTACTATGGAGCCAGACAGCAGACCTGAA CTGCAGGAGAAAGCAGCGTTACTGAAGAAAACACTTGAAAATCTGGAATAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001080398
Insert Size	6054 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001080398.1
RefSeq Size	7391 bp
RefSeq ORF	6054 bp
Locus ID	23392
MW	223.7 kDa
Gene Summary	Adapter/scaffolding protein that binds to the 26S proteasome, motor proteins and other compartment specific proteins. May couple the proteasome to different compartments including endosome, endoplasmic reticulum and centrosome. May play a role in ERAD and other enhanced proteolyis (PubMed:15496406). Promotes proteasome dissociation under oxidative stress (By similarity).[UniProtKB/Swiss-Prot Function]

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