USP35 (NM_020798) Human Untagged Clone
CAT＃: SC316437
USP35 (untagged)-Human ubiquitin specific peptidase 35 (USP35)
CNY 19570.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC316437 representing NM_020798.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGACAAGATCTTGGAGGCGGTGGTGACGTCGTCATACCCGGTCAGCGTGAAGCAGGGGCTGGTTCGG CGCGTGCTGGAGGCGGCGCGGCAGCCGCTGGAGCGTGAGCAGTGCCTGGCGCTGCTGGCGCTGGGCGCG CGCCTCTACGTGGGCGGCGCGGAGGAGCTGCCGCGCCGCGTGGGCTGCCAGCTGCTGCACGTGGCCGGC CGCCACCACCCCGACGTCTTCGCCGAGTTCTTCAGCGCGCGTCGCGTGCTGCGCCTGCTGCAGGGTGGC GCCGGCCCCCCGGGCCCCCGCGCGCTCGCCTGCGTGCAGCTGGGTCTGCAGCTGCTGCCCGAGGGGCCT GCGGCCGACGAGGTGTTCGCGCTGCTGCGGCGCGAGGTGCTGCGCACCGTGTGCGAGCGCCCGGGCCCC GCGGCCTGCGCGCAGGTGGCACGGCTGCTGGCTCGCCACCCGCGCTGTGTGCCCGACGGACCCCACCGC CTGCTCTTCTGCCAGCAGCTGGTGCGTTGCCTCGGCCGCTTCCGCTGCCCAGCCGAAGGCGAGGAGGGC GCCGTGGAGTTCCTAGAGCAGGCCCAGCAGGTGAGCGGGCTCCTGGCGCAGCTGTGGCGCGCACAGCCC GCCGCCATCCTGCCCTGCCTCAAAGAGCTGTTCGCAGTCATCTCCTGCGCAGAGGAGGAGCCACCATCT AGCGCCCTGGCCAGCGTGGTCCAGCACCTCCCATTGGAGCTCATGGATGGTGTTGTCCGGAACCTCAGC AATGATGACAGTGTGACAGACTCGCAGATGCTGACTGCCATTAGCAGGATGATTGACTGGGTGTCCTGG CCCCTGGGGAAGAATATTGACAAGTGGATCATTGCACTGCTGAAGGGCCTGGCTGCTGTTAAGAAGTTC AGCATCTTGATCGAGGTTTCGCTCACCAAAATTGAGAAGGTTTTCTCTAAGCTGCTGTACCCCATCGTC CGGGGAGCTGCCTTGTCTGTGCTCAAGTACATGCTCCTGACCTTCCAGCACTCCCACGAAGCCTTCCAC CTGCTCCTCCCTCACATCCCCCCCATGGTGGCCTCTCTGGTCAAGGAGGACTCGAACTCGGGGACCAGC TGCCTGGAGCAGCTGGCGGAGCTGGTCCACTGCATGGTGTTCCGGTTCCCGGGCTTCCCGGATCTGTAT GAGCCTGTCATGGAGGCCATCAAGGACCTCCATGTTCCCAATGAGGACCGCATCAAGCAGCTGCTGGGG CAGGATGCCTGGACTTCGCAGAAGAGCGAGCTGGCGGGTTTCTATCCCCGGCTCATGGCCAAGTCAGAC ACGGGCAAGATTGGTCTCATCAACCTGGGCAACACATGCTATGTCAACAGCATCCTTCAGGCCTTATTC ATGGCGTCTGACTTCAGACATTGTGTGCTCCGCTTGACTGAGAACAACTCACAGCCCCTGATGACCAAG CTGCAGTGGCTCTTTGGCTTCCTAGAACACAGCCAGCGGCCTGCCATTTCCCCAGAGAACTTCCTCTCC GCATCCTGGACGCCCTGGTTCAGCCCTGGCACCCAGCAGGACTGCTCGGAGTATCTGAAGTACCTGCTG GATCGGCTGCACGAAGAGGAGAAAACGGGCACAAGGATCTGCCAGAAACTCAAGCAGTCCAGCTCGCCC TCTCCGCCCGAGGAGCCCCCGGCCCCAAGTTCAACCTCTGTGGAAAAAATGTTTGGAGGCAAGATAGTG ACTCGGATCTGCTGTCTCTGCTGCCTCAACGTCTCCTCCCGGGAGGAGGCCTTCACGGACCTCTCTCTC GCCTTCCCTCCTCCTGAGCGCTGTCGCCGCCGCCGCCTGGGCTCTGTGATGCGCCCCACAGAAGACATC ACAGCCCGGGAGTTGCCCCCACCAACCAGTGCACAGGGGCCAGGCAGGGTGGGTCCTCGGAGGCAAAGG AAACACTGCATCACAGAGGACACCCCCCCCACCAGCCTGTACATCGAAGGCCTGGACTCCAAGGAAGCT GGTGGGCAGAGCAGTCAGGAGGAAAGGATAGAGAGGGAGGAAGAAGGGAAGGAGGAGAGAACGGAGAAG GAAGAAGTGGGGGAGGAGGAGGAAAGCACCAGAGGGGAAGGAGAGAGGGAGAAAGAGGAGGAGGTGGAA GAGGAAGAAGAGAAGGTGGAGAAGGAGACAGAAAAGGAGGCTGAGCAGGAAAAGGAAGAAGACAGCCTG GGAGCGGGGACCCACCCGGATGCTGCCATCCCCTCCGGGGAGCGGACATGTGGCTCTGAGGGCTCCCGC TCCGTCCTGGACCTGGTTAACTACTTCCTGTCCCCCGAGAAGCTGACAGCAGAAAACCGCTACTACTGC GAGTCGTGTGCCTCCCTGCAGGATGCCGAGAAGGTGGTGGAGCTGAGCCAAGGGCCGTGCTACCTCATC CTCACACTGCTGCGCTTCTCTTTCGACCTGCGCACCATGCGGCGCCGCAAGATCCTGGATGACGTCTCC ATCCCCCTGCTGCTCCGCCTGCCACTGGCTGGTGGCCGTGGCCAGGCCTATGACCTCTGCAGTGTGGTG GTGCACTCTGGAGTGTCTTCGGAGAGTGGTCACTACTACTGCTATGCCCGTGAGGGCGCTGCCCGCCCT GCCGCTTCTCTGGGAACTGCCGATAGGCCAGAGCCCGAGAACCAGTGGTACCTGTTCAATGACACTCGG GTGTCCTTCTCTTCCTTCGAATCTGTCAGCAACGTCACCTCCTTCTTCCCTAAGGACACAGCCTATGTG CTGTTTTACCGGCAGCGGCCCAGGGAGGGGCCCGAGGCTGAGTTGGGCTCTTCTAGAGTCCGGACAGAG CCCACCCTGCACAAGGACTTGATGGAAGCCATTTCCAAAGACAACATCCTTTACCTACAGGAGCAGGAG AAGGAGGCCCGGAGCAGGGCGGCCTACATCTCTGCACTCCCCACATCTCCGCACTGGGGGAGGGGCTTT GATGAAGACAAGGATGAGGATGAAGGCTCTCCAGGGGGCTGCAATCCTGCAGGTGGCAATGGTGGTGAC TTCCACAGACTGGTCTTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_020798 |
| Insert Size | 3057 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020798.2 |
| RefSeq Size | 4311 bp |
| RefSeq ORF | 3057 bp |
| Locus ID | 57558 |
| UniProt ID | Q9P2H5 |
| Protein Families | Druggable Genome, Protease |
| MW | 113.4 kDa |
| Gene Summary | This gene encodes a member of the peptidase C19 family of ubiquitin-specific proteases. These deubiquitinating enzymes (DUBs) catalyze the removal of ubiquitin proteins from other proteins. The encoded protein associates with polarized mitochondria and has been shown to inhibit NF-kappa B activation and delay PARK2-mediated degradation of mitochondria. Expression of this gene is upregulated by the let-7a microRNA and reduced expression has been observed in human tumor tissues. [provided by RefSeq, Jul 2017] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219955 | USP35 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 35 (USP35) |
CNY 7088.00 |
|
| RC219955L1 | Lenti ORF clone of Human ubiquitin specific peptidase 35 (USP35), Myc-DDK-tagged |
CNY 9488.00 |
|
| RC219955L2 | Lenti ORF clone of Human ubiquitin specific peptidase 35 (USP35), mGFP tagged |
CNY 12070.00 |
|
| RC219955L3 | Lenti ORF clone of Human ubiquitin specific peptidase 35 (USP35), Myc-DDK-tagged |
CNY 12070.00 |
|
| RC219955L4 | Lenti ORF clone of Human ubiquitin specific peptidase 35 (USP35), mGFP tagged |
CNY 12070.00 |
|
| RG219955 | USP35 (tGFP-tagged) - Human ubiquitin specific peptidase 35 (USP35) |
CNY 11210.00 |
