SGCE (NM_001099401) Human Untagged Clone
CAT＃: SC316529
SGCE (untagged)-Human sarcoglycan, epsilon (SGCE), transcript variant 1
CNY 3656.00
CNY 7320.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DYT11; epsilon-SG; ESG |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>SC316529 representing NM_001099401.
Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCAATTGCCCCGGTGGTGGGAGCTGGGAGACCCCTGTGCTTGGACGGGACAGGGTCGGGGGACACGC AGGATGAGCCCCGCGACCACTGGCACATTCTTGCTGACAGTGTACAGTATTTTCTCCAAGGTACACTCC GATCGGAATGTATACCCATCAGCAGGTGTCCTCTTTGTTCATGTTTTGGAAAGAGAATATTTTAAGGGG GAATTTCCACCTTACCCAAAACCTGGCGAGATTAGTAATGATCCCATAACATTTAATACAAATTTAATG GGTTACCCAGACCGACCTGGATGGCTTCGATATATCCAAAGGACACCATATAGTGATGGAGTCCTATAT GGGTCCCCAACAGCTGAAAATGTGGGGAAGCCAACAATCATTGAGATAACTGCCTACAACAGGCGCACC TTTGAGACTGCAAGGCATAATTTGATAATTAATATAATGTCTGCAGAAGACTTCCCGTTGCCATATCAA GCAGAATTCTTCATTAAGAATATGAATGTAGAAGAAATGTTGGCCAGTGAGGTTCTTGGAGACTTTCTT GGCGCAGTGAAAAATGTGTGGCAGCCAGAGCGCCTGAACGCCATAAACATCACATCGGCCCTAGACAGG GGTGGCAGGGTGCCACTTCCCATTAATGACCTGAAGGAGGGCGTTTATGTCATGGTTGGTGCAGATGTC CCGTTTTCTTCTTGTTTACGAGAAGTTGAAAATCCACAGAATCAATTGAGATGTAGTCAAGAAATGGAG CCTGTAATAACATGTGATAAAAAATTTCGTACTCAATTTTACATTGACTGGTGCAAAATTTCATTGGTT GATAAAACAAAGCAAGTGTCCACCTATCAGGAAGTGATTCGTGGAGAGGGGATTTTACCTGATGGTGGA GAATACAAACCCCCTTCTGATTCTTTGAAAAGCAGAGACTATTACACGGATTTCCTAATTACACTGGCT GTGCCCTCGGCAGTGGCACTGGTCCTTTTTCTAATACTTGCTTATATCATGTGCTGCCGACGGGAAGGC GTGGAAAAGAGAAACATGCAAACACCAGACATCCAACTGGTCCATCACAGTGCTATTCAGAAATCTACC AAGGAGCTTCGAGACATGTCCAAGAATAGAGAGATAGCATGGCCCCTGTCAACGCTTCCTGTGTTCCAC CCTGTGACTGGGGAAATCATACCTCCTTTACACACAGACAACTATGATAGCACAAACATGCCATTGATG CAAACGCAGCAATGGAGTTTCGCTCCTGTTGCCCAGGCTGGAGTGCAATGGAGCGATCTTGGCTCACTG CAGCCTCCGCCTCCCAGGAACTTGCCACATCAGACTCAGATTCCCCAACAGCAGACTACAGGTAAATGG TATCCCTGA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001099401 |
| Insert Size | 1389 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001099401.1. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001099401.1 |
| RefSeq Size | 1784 bp |
| RefSeq ORF | 1389 bp |
| Locus ID | 8910 |
| UniProt ID | O43556 |
| Protein Families | Transmembrane |
| MW | 52.5 kDa |
| Gene Summary | This gene encodes the epsilon member of the sarcoglycan family. Sarcoglycans are transmembrane proteins that are components of the dystrophin-glycoprotein complex, which link the actin cytoskeleton to the extracellular matrix. Unlike other family members which are predominantly expressed in striated muscle, the epsilon sarcoglycan is more broadly expressed. Mutations in this gene are associated with myoclonus-dystonia syndrome. This gene is imprinted, with preferential expression from the paternal allele. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. A pseudogene associated with this gene is located on chromosome 2. [provided by RefSeq, Oct 2016] Transcript Variant: This variant (1) lacks an alternate in-frame exon in the 5' coding region and contains an additional in-frame exon in the 3' coding region, compared to variant 5. It encodes isoform 1, which shares the same termini, but is shorter than isoform 5. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
SGCE missense mutations that cause myoclonus-dystonia syndrome impair {varepsilon}-sarcoglycan trafficking to the plasma membrane: modulation by ubiquitination and torsinA,
,Christopher T. Esapa, Adrian Waite, Matthew Locke, Matthew A. Benson, Michaela Kraus, R.A. Jeffrey McIlhinney, Roy V. Sillitoe, Philip W. Beesley, Derek J. Blake,
Hum Mol Genet. 2007 Feb 1;16(3):327-42.
[SGCE]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC217105 | SGCE (Myc-DDK-tagged)-Human sarcoglycan, epsilon (SGCE), transcript variant 1 |
CNY 3656.00 |
|
| RC217105L3 | Lenti ORF clone of Human sarcoglycan, epsilon (SGCE), transcript variant 1, Myc-DDK-tagged |
CNY 5990.00 |
|
| RC217105L4 | Lenti ORF clone of Human sarcoglycan, epsilon (SGCE), transcript variant 1, mGFP tagged |
CNY 5990.00 |
|
| RG217105 | SGCE (tGFP-tagged) - Human sarcoglycan, epsilon (SGCE), transcript variant 1 |
CNY 4470.00 |
