MRPL19 (NM_014763) Human Untagged Clone

CAT#: SC317426

MRPL19 (untagged)-Human mitochondrial ribosomal protein L19 (MRPL19), nuclear gene encoding mitochondrial protein



  "NM_014763" in other vectors (6)

CNY 6,270.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
MRPL19 Antibody - C-terminal region
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms L19mt; MRP-L15; MRP-L19; MRPL15; RLX1; RPML15
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC317426 representing NM_014763.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGGCGGCCTGCATTGCAGCGGGGCACTGGGCTGCAATGGGCCTAGGCCGGAGTTTCCAAGCCGCCAGG
ACTCTGCTCCCCCCGCCGGCCTCTATCGCCTGCAGGGTCCACGCGGGGCCTGTCCGGCAGCAGAGCACT
GGGCCTTCCGAGCCCGGTGCGTTCCAACCGCCGCCGAAACCGGTCATCGTGGACAAGCACCGCCCCGTG
GAACCGGAACGCAGGTTCTTGAGTCCTGAATTCATTCCTCGAAGGGGAAGAACAGATCCTCTGAAATTT
CAAATAGAAAGAAAAGATATGTTAGAAAGGAGAAAAGTACTCCACATTCCAGAGTTCTATGTTGGAAGT
ATTCTTCGTGTTACTACAGCTGACCCATATGCCAGTGGAAAAATCAGCCAGTTTCTGGGGATTTGCATT
CAGAGATCAGGAAGAGGACTTGGAGCTACTTTCATCCTTAGGAATGTTATCGAAGGACAAGGTGTCGAG
ATTTGCTTTGAACTTTATAATCCTCGGGTCCAGGAGATTCAGGTGGTCAAATTAGAGAAACGGCTGGAT
GATAGCTTGCTATACTTACGAGATGCCCTTCCTGAATATAGCACTTTTGATGTGAATATGAAGCCAGTA
GTACAAGAGCCTAACCAAAAAGTTCCTGTTAATGAGCTGAAAGTAAAAATGAAGCCTAAGCCCTGGTCT
AAACGCTGGGAACGTCCAAATTTTAATATTAAAGGAATCAGATTTGATCTTTGTTTAACTGAACAGCAA
ATGAAAGAAGCTCAGAAGTGGAATCAGCCATGGCTTGAATTTGATATGATGAGGGAATATGATACTTCA
AAAATTGAAGCTGCAATATGGAAGGAAATTGAAGCGTCGAAAAGGTCTTGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_014763
Insert Size 879 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014763.3
RefSeq Size 7827 bp
RefSeq ORF 879 bp
Locus ID 9801
UniProt ID P49406
Domains Ribosomal_L19
MW 33.5 kDa
Gene Summary Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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