RASGEF1A (NM_145313) Human Untagged Clone
CAT＃: SC317802
RASGEF1A (untagged)-Human RasGEF domain family, member 1A (RASGEF1A)
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CG4853 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC317802 representing NM_145313.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCCCAGACGTCCGTTGTCTTCTCCAGCATCCTTGGGCCCAGCTGTAGCGGACAGGTGCAGCCTGGC ATGGGGGAGCGTGGAGGCGGGGCCGGTGGCGGCTCCGGGGACCTCATCTTCCAAGATGGACACCTCATC TCTGGGTCCCTGGAGGCCCTGATGGAGCACCTTGTTCCCACGGTGGACTATTACCCCGATAGGACGTAC ATCTTCACCTTTCTCCTGAGCTCCCGGGTCTTTATGCCCCCTCATGACCTGCTGGCCCGCGTGGGGCAG ATCTGCGTGGAGCAGAAGCAGCAGCTGGAAGCCGGGCCTGAAAAGGCCAAGCTGAAGTCTTTCTCAGCC AAGATCGTGCAGCTCCTGAAGGAGTGGACCGAGGCCTTCCCCTATGACTTCCAGGATGAGAAGGCCATG GCCGAGCTGAAAGCCATCACACACCGTGTCACCCAGTGTGATGAGGAGAATGGCACAGTGAAGAAGGCC ATTGCCCAGATGACACAGAGCCTGTTGCTGTCCTTGGCTGCCCGGAGCCAGCTCCAGGAACTGCGAGAG AAGCTCCGGCCACCGGCTGTAGACAAGGGGCCCATCCTCAAGACCAAGCCACCAGCCGCCCAGAAGGAC ATCCTGGGCGTGTGCTGCGACCCCCTGGTGCTGGCCCAGCAGCTGACTCACATTGAGCTGGACAGGGTC AGCAGCATTTACCCTGAGGACTTGATGCAGATCGTCAGCCACATGGACTCCTTGGACAACCACAGGTGC CGAGGGGACCTGACCAAGACCTACAGCCTGGAGGCCTATGACAACTGGTTCAACTGCCTGAGCATGCTG GTGGCCACTGAGGTGTGCCGGGTGGTGAAGAAGAAACACCGGACCCGCATGTTGGAGTTCTTCATTGAT GTGGCCCGGGAGTGCTTCAACATCGGGAACTTCAACTCCATGATGGCCATCATCTCTGGCATGAACCTC AGTCCTGTGGCAAGGCTGAAGAAAACTTGGTCCAAGGTCAAGACAGCCAAGTTTGATGTCTTGGAGCAT CACATGGACCCGTCCAGCAACTTCTGCAACTACCGTACAGCCCTGCAGGGGGCCACGCAGAGGTCCCAG ATGGCCAACAGCAGCCGTGAAAAGATCGTCATCCCTGTGTTCAACCTCTTCGTTAAGGACATCTACTTC CTGCACAAAATCCATACCAACCACCTGCCCAACGGGCACATTAACTTTAAGAAATTCTGGGAGATCTCC AGACAGATCCATGAGTTCATGACATGGACACAGGTAGAGTGTCCTTTCGAGAAGGACAAGAAGATTCAG AGTTACCTGCTCACGGCGCCCATCTACAGCGAGGAAGCTCTCTTCGTCGCCTCCTTTGAAAGTGAGGGT CCCGAGAACCACATGGAAAAAGACAGCTGGAAGACCCTCAGGACCACCCTTCTGAACAGAGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_145313 |
| Insert Size | 1446 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_145313.3 |
| RefSeq Size | 3243 bp |
| RefSeq ORF | 1446 bp |
| Locus ID | 221002 |
| UniProt ID | Q8N9B8 |
| MW | 54.6 kDa |
| Gene Summary | Guanine nucleotide exchange factor (GEF) with specificity for RAP2A, KRAS, HRAS, and NRAS (in vitro). Plays a role in cell migration.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate 5' terminal exon and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC205498 | RASGEF1A (Myc-DDK-tagged)-Human RasGEF domain family, member 1A (RASGEF1A) |
CNY 3656.00 |
|
| RC205498L3 | Lenti ORF clone of Human RasGEF domain family, member 1A (RASGEF1A), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC205498L4 | Lenti ORF clone of Human RasGEF domain family, member 1A (RASGEF1A), mGFP tagged |
CNY 5890.00 |
|
| RG205498 | RASGEF1A (tGFP-tagged) - Human RasGEF domain family, member 1A (RASGEF1A) |
CNY 5256.00 |
其它RASGEF1A产品
