EML1 (NM_004434) Human Untagged Clone
CAT＃: SC318296
EML1 (untagged)-Human echinoderm microtubule associated protein like 1 (EML1), transcript variant 2
CNY 7220.00
Product images
CNY 6281.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | BH; ELP79; EMAP; EMAP-1; EMAPL |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318296 representing NM_004434.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAGGACGGCTTCTCCAGCTACAGCAGCCTGTACGACACGTCCTCGCTGCTCCAGTTCTGCAACGAT GACAGCGCTTCTGCTGCAAGTAGCATGGAGGTGACAGACCGCATTGCTTCACTGGAGCAGAGAGTCCAG ATGCAAGAAGACGACATCCAGCTGCTCAAATCAGCTCTAGCTGATGTGGTTCGGCGGCTGAACATTACT GAGGAACAGCAGGCCGTGCTTAACAGGAAAGGACCTACCAAAGCAAGACCACTGATGCAGACCCTGCCT TTAAGAACCACGGTCAACAATGGCACTGTGTTACCAAAGAAACCTACTGGCTCTCTACCATCCCCCTCC GGGGTCAGGAAAGAAACTGCTGTGCCAGCAACCAAAAGTAACATCAAGAGGACCAGCTCTTCTGAACGA GTGTCTCCTGGGGGTCGAAGGGAAAGCAATGGGGATTCCAGAGGAAACCGGAATCGCACAGGCTCCACC AGCAGCTCTTCCAGTGGCAAAAAGAACAGTGAAAGCAAACCCAAGGAGCCTGTATTCAGTGCAGAAGAA GGCTATGTAAAAATGTTTCTTCGTGGACGCCCTGTTACCATGTACATGCCCAAAGATCAAGTGGATTCT TACAGCTTGGAAGCAAAAGTAGAACTTCCAACCAAGAGACTCAAGCTGGAATGGGTCTATGGGTACAGG GGTCGAGACTGCCGTAACAACCTGTACTTGCTTCCGACGGGAGAGACCGTCTACTTCATCGCATCCGTG GTGGTGTTATACAACGTGGAGGAGCAACTGCAGAGGCATTACGCTGGCCACAACGATGACGTGAAGTGC CTAGCAGTTCATCCTGATCGGATCACGATAGCAACAGGACAAGTTGCGGGCACATCGAAGGATGGAAAA CAATTGCCCCCACATGTGCGCATCTGGGATTCTGTGACATTGAATACTCTCCACGTCATTGGAATAGGT TTTTTTGACCGAGCAGTCACCTGTATTGCATTCTCAAAATCTAATGGAGGAACCAATCTCTGTGCTGTG GATGACTCCAACGACCATGTGCTCTCTGTATGGGACTGGCAGAAAGAAGAAAAACTAGCAGATGTGAAG TGCTCTAATGAAGCTGTGTTTGCTGCGGATTTCCACCCCACGGACACCAACATCATAGTTACTTGTGGA AAATCACATCTCTACTTTTGGACACTAGAAGGAAGCTCCCTTAATAAGAAGCAAGGATTATTCGAGAAA CAAGAAAAGCCAAAGTTTGTCCTCTGTGTGACTTTCTCTGAAAACGGTGACACCATTACTGGAGATTCA AGTGGCAACATCTTAGTATGGGGAAAAGGTACAAATCGAATAAGCTATGCAGTTCAGGGGGCCCATGAG GGTGGCATTTTTGCACTTTGTATGTTAAGAGATGGCACACTGGTGTCGGGAGGTGGGAAAGACCGAAAG CTCATTTCTTGGAGCGGAAACTATCAAAAACTTCGTAAAACGGAGATTCCAGAACAGTTTGGTCCAATA CGGACAGTGGCCGAGGGGAAAGGCGATGTGATCTTGATTGGCACAACTCGAAACTTTGTCCTGCAGGGC ACTCTGTCAGGGGACTTCACACCCATTACTCAGGGTCACACTGATGAGCTCTGGGGACTGGCCATCCAT GCCTCAAAATCTCAGTTCTTGACCTGTGGGCATGACAAGCATGCCACTCTCTGGGACGCTGTGGGTCAC CGTCCCGTCTGGGACAAAATAATAGAGGATCCAGCTCAGTCTTCTGGTTTTCATCCTTCAGGGTCTGTG GTTGCAGTCGGAACACTCACTGGGAGGTGGTTTGTGTTTGACACAGAAACAAAAGACTTGGTCACCGTT CACACAGATGGAAACGAACAGCTCTCTGTAATGCGATACTCACCAGATGGGAATTTCTTAGCCATAGGC TCACATGACAACTGCATCTATATATATGGCGTTAGTGACAACGGGAGGAAGTACACGCGAGTGGGCAAG TGCTCGGGTCATTCCAGCTTCATTACTCACCTGGACTGGTCTGTAAACTCACAGTTCCTCGTGTCAAAT TCCGGAGACTACGAAATCCTCTACTGGGTTCCCTCTGCCTGTAAGCAAGTCGTAAGTGTGGAAACTACA AGAGACATTGAATGGGCTACCTATACCTGCACTTTGGGATTCCATGTTTTTGGAGTGTGGCCAGAAGGC TCGGACGGAACCGACATCAATGCCGTCTGTCGGGCCCATGAGAAGAAACTCCTGTCAACAGGCGACGAC TTTGGCAAAGTGCACCTCTTCTCATACCCCTGCTCGCAGTTCAGGGCTCCAAGCCACATCTACGGCGGG CACAGCAGCCATGTCACCAATGTCGATTTCCTCTGTGAAGACAGCCACCTCATCTCCACGGGCGGGAAA GACACAAGCATCATGCAGTGGCGCGTCATTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_004434 |
| Insert Size | 2448 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_004434.2 |
| RefSeq Size | 4479 bp |
| RefSeq ORF | 2448 bp |
| Locus ID | 2009 |
| UniProt ID | O00423 |
| Domains | WD40, HELP |
| MW | 89.9 kDa |
| Gene Summary | Human echinoderm microtubule-associated protein-like is a strong candidate for the Usher syndrome type 1A gene. Usher syndromes (USHs) are a group of genetic disorders consisting of congenital deafness, retinitis pigmentosa, and vestibular dysfunction of variable onset and severity depending on the genetic type. The disease process in USHs involves the entire brain and is not limited to the posterior fossa or auditory and visual systems. The USHs are catagorized as type I (USH1A, USH1B, USH1C, USH1D, USH1E and USH1F), type II (USH2A and USH2B) and type III (USH3). The type I is the most severe form. Gene loci responsible for these three types are all mapped. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206372 | EML1 (Myc-DDK-tagged)-Human echinoderm microtubule associated protein like 1 (EML1), transcript variant 2 |
CNY 5968.00 |
|
| RC206372L3 | Lenti ORF clone of Human echinoderm microtubule associated protein like 1 (EML1), transcript variant 2, Myc-DDK-tagged |
CNY 7700.00 |
|
| RC206372L4 | Lenti ORF clone of Human echinoderm microtubule associated protein like 1 (EML1), transcript variant 2, mGFP tagged |
CNY 7700.00 |
|
| RG206372 | EML1 (tGFP-tagged) - Human echinoderm microtubule associated protein like 1 (EML1), transcript variant 2 |
CNY 7568.00 |
|
| SC117349 | EML1 (untagged)-Human echinoderm microtubule associated protein like 1 (EML1), transcript variant 2 |
CNY 10216.00 |
