EFTUD1 (EFL1) (NM_024580) Human Untagged Clone
CAT＃: SC318504
EFTUD1 (untagged)-Human elongation factor Tu GTP binding domain containing 1 (EFTUD1), transcript variant 1
CNY 7808.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | EFTUD1; FAM42A; HsT19294; RIA1; SDS2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318504 representing NM_024580.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGTGCTCAACAGTTTGGATAAGATGATTCAACTCCAGAAAAACACTGCCAACATCAGGAATATTTGT GTTTTGGCTCATGTTGACCATGGAAAAACTACTCTGGCTGACTGTCTTATATCTAGCAATGGAATCATC TCCAGCCGCCTAGCAGGCAAGTTAAGGTACATGGACAGCAGAGAAGATGAACAGATCCGAGGGATCACT ATGAAATCCAGTGCCATTTCCCTACATTATGCAACAGGTAATGAGGAGTACCTGATCAATCTGATAGAC TCTCCAGGACACGTGGACTTTTCCTCAGAAGTATCAACCGCTGTTCGCATTTGTGATGGATGCATCATT GTGGTAGATGCTGTGGAAGGAGTCTGTCCACAGACACAGGCAGTTCTGCGACAAGCTTGGCTTGAAAAC ATCCGTCCGGTTTTAGTGATTAATAAGATTGATCGCTTGATAGTGGAACTGAAATTCACCCCACAAGAG GCCTATTCTCACCTCAAGAATATTTTAGAACAGATTAATGCGCTCACAGGGACTCTTTTTACTTCTAAA GTCCTAGAAGAAAGAGCAGAGAGGGAGACTGAATCCCAAGTGAATCCAAATTCTGAACAAGGAGAGCAA GTATATGACTGGAGCACTGGCTTGGAGGACACAGATGATTCTCACCTTTACTTCTCTCCAGAACAGGGA AATGTGGTGTTTACCAGTGCAATAGATGGGTGGGGCTTTGGAATTGAGCACTTCGCCAGAATCTACAGT CAAAAAATTGGCATCAAAAAGGAAGTTCTTATGAAAACCTTGTGGGGAGATTACTATATAAATATGAAG GCTAAAAAGATCATGAAGGGTGATCAGGCCAAAGGAAAGAAACCTTTATTTGTACAGTTGATCCTGGAA AATATATGGAGTTTGTATGATGCTGTTTTGAAAAAGGACAAAGACAAAATTGATAAAATAGTGACTTCT TTAGGATTAAAAATTGGAGCCCGGGAGGCACGACATTCAGACCCTAAAGTTCAGATCAACGCCATTTGC AGTCAGTGGCTACCCATATCCCATGCTGTTCTTGCTATGGTGTGTCAGAAACTTCCTAGTCCCCTTGAT ATTACAGCTGAGAGAGTGGAGAGACTGATGTGCACAGGATCACAAACTTTTGACTCTTTTCCACCAGAA ACTCAAGCACTGAAAGCAGCTTTTATGAAATGTGGAAGTGAGGACACTGCTCCAGTTATTATATTTGTT TCCAAAATGTTTGCAGTTGATGCTAAGGCCTTGCCTCAGAATAAGCCAAGGCCTCTCACTCAAGAAGAA ATTGCTCAGAGACGTGAGCGTGCAAGACAAAGGCATGCAGAGAAGCTTGCAGCAGCACAGGGACAGGCA CCCTTGGAGCCCACCCAAGATGGGAGTGCCATTGAAACATGTCCAAAAGGAGAGGAGCCAAGAGGTGAC GAGCAACAGGTGGAAAGTATGACCCCTAAACCTGTGCTCCAGGAAGAAAACAACCAAGAGTCTTTTATT GCATTTGCTCGGGTGTTCAGTGGTGTGGCTCGAAGAGGAAAGAAAATTTTTGTCTTGGGGCCCAAATAC AGTCCTCTTGAGTTTTTACGAAGGGTACCATTAGGCTTCTCAGCTCCACCAGATGGCCTCCCCCAAGTC CCCCACATGGCATACTGTGCTCTGGAAAACCTGTATCTTCTGATGGGAAGGGAACTGGAATATCTAGAG GAGGTACCTCCAGGAAATGTGCTAGGAATAGGAGGCCTTCAAGATTTTGTGCTGAAATCTGCAACACTG TGTAGCCTGCCATCCTGCCCACCATTTATACCACTCAACTTCGAAGCCACTCCTATTGTGAGAGTTGCT GTTGAACCAAAACATCCAAGTGAAATGCCTCAGCTCGTAAAAGGAATGAAACTGTTAAACCAGGCTGAT CCCTGTGTCCAGATTTTAATTCAGGAAACGGGAGAGCACGTTTTAGTCACAGCAGGAGAAGTCCACCTT CAGCGATGCCTGGATGACTTAAAAGAAAGGTTTGCAAAGATTCATATCAGTGTATCTGAACCTATTATT CCATTCAGAGAAACAATCACAAAACCCCCAAAAGTTGACATGGTCAATGAAGAAATAGGCAAACAGCAA AAAGTTGCAGTCATACACCAAATGAAAGAAGATCAAAGCAAAATCCCTGAAGGAATCCAAGTTGACTCT GACGGGCTAATCACCATAACAACTCCCAATAAACTTGCCACGCTCAGTGTTCGAGCCATGCCCCTTCCA GAAGAAGTCACCCAGATTCTGGAAGAAAATAGTGATTTGATTCGTTCTATGGAGCAGTTGACATCCTCT TTGAATGAGGGTGAAAATACTCACATGATTCATCAGAAGACCCAAGAGAAAATTTGGGAATTCAAAGGA AAACTGGAGCAACACCTAACAGGGAGAAGATGGAGGAACATTGTTGACCAAATCTGGTCATTTGGCCCA AGAAAATGTGGGCCCAACATACTAGTCAATAAAAGTGAAGATTTTCAGAACTCAGTATGGACAGGTCCA GCTGACAAAGCTTCAAAAGAAGCCAGTAGATACCGAGATTTGGGCAATAGCATTGTGAGTGGCTTCCAA CTAGCAACCCTCTCTGGCCCCATGTGTGAGGAGCCTCTCATGGGTGTCTGTTTTGTTCTGGAAAAATGG GACCTAAGTAAATTTGAGGAACAAGGAGCAAGTGATCTGGCAAAAGAGGGACAGGAGGAAAATGAAACC TGTTCTGGTGGAAATGAAAACCAAGAGCTACAAGATGGCTGCTCTGAGGCCTTTGAGAAGAGGACATCA CAGAAAGGAGAATCTCCACTCACTGACTGCTATGGACCTTTCTCAGGACAGCTAATTGCCACCATGAAA GAAGCATGTCGCTATGCACTGCAAGTGAAACCTCAGCGCCTGATGGCAGCTATGTACACATGTGACATC ATGGCCACTGGTGATGTTCTCGGTCGAGTCTATGCTGTCTTGTCAAAGAGAGAAGGTCGGGTACTTCAA GAAGAAATGAAAGAAGGGACAGACATGTTCATCATCAAGGCTGTGCTGCCTGTTGCTGAAAGCTTTGGT TTTGCTGATGAAATCAGGAAGAGGACAAGTGGCCTGGCCAGCCCACAACTAGTATTCAGCCATTGGGAG ATCATTCCCAGTGACCCCTTCTGGGTGCCAACTACTGAGGAGGAATACTTGCACTTTGGGGAGAAGGCT GACTCTGAGAACCAAGCCCGGAAGTACATGAACGCAGTACGAAAGCGGAAGGGGCTTTATGTGGAAGAA AAGATTGTGGAGCATGCAGAAAAGCAGAGGACACTCAGCAAAAATAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_024580 |
| Insert Size | 3363 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_024580.5 |
| RefSeq Size | 3685 bp |
| RefSeq ORF | 3363 bp |
| Locus ID | 79631 |
| UniProt ID | Q7Z2Z2 |
| Domains | EFG_C |
| MW | 125.4 kDa |
| Gene Summary | Involved in the biogenesis of the 60S ribosomal subunit and translational activation of ribosomes. Together with SBDS, triggers the GTP-dependent release of EIF6 from 60S pre-ribosomes in the cytoplasm, thereby activating ribosomes for translation competence by allowing 80S ribosome assembly and facilitating EIF6 recycling to the nucleus, where it is required for 60S rRNA processing and nuclear export. Has low intrinsic GTPase activity. GTPase activity is increased by contact with 60S ribosome subunits.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the the longest isoform (1). |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221410 | EFTUD1 (Myc-DDK-tagged)-Human elongation factor Tu GTP binding domain containing 1 (EFTUD1), transcript variant 1 |
CNY 11210.00 |
|
| RC221410L3 | Lenti-ORF clone of EFTUD1 (Myc-DDK-tagged)-Human elongation factor Tu GTP binding domain containing 1 (EFTUD1), transcript variant 1 |
CNY 13110.00 |
|
| RC221410L4 | Lenti-ORF clone of EFTUD1 (mGFP-tagged)-Human elongation factor Tu GTP binding domain containing 1 (EFTUD1), transcript variant 1 |
CNY 13110.00 |
|
| RG221410 | EFTUD1 (tGFP-tagged) - Human elongation factor Tu GTP binding domain containing 1 (EFTUD1), transcript variant 1 |
CNY 12350.00 |
