DENND4B (NM_014856) Human Untagged Clone
CAT＃: SC318637
DENND4B (untagged)-Human DENN/MADD domain containing 4B (DENND4B)
CNY 28790.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | KIAA0476 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318637 representing NM_014856.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGGAGGAGCGGCCCCCCCGGCTGGTGGATTACTTCGTGGTAGCTGGGCTTGCAGGGAACGGAGCA CCCATCCCTGAGGAAACGTGGGTTCCTGAACCCAGTGGGCCCCTGCGCCCTCCCCGGCCAGCTGAGCCC ATCACAGATGTGGCAGTCATCGCTAGGGCACTGGGCGAGGAAGTGCCCCAGGGCTACACATGCATCCAG GCTTCTGCTGGGGGCCACCCCTTGGAACTCAGTGCTGGACTTCTGGGTGGAACTCAACCCGTCATCTGC TACCGCAGGGGCCGTGACAAGCCCCCCCTCGTTGAGCTGGGGGTGTTGTATGAGGGGAAGGAACGTCCC AAGCCTGGCTTCCAAGTGCTTGACACGACACCCTACAGCCACTCAGCAAACCTGGCCCCTCCAGGCCCC GGGCACCCCCGCACCTACCTCACTTACCGGCGGGCAGCAGAGGGGGCAGGGCTGCATGCCCTGGGCATC ACTGACCTCTGCCTGGTGCTGCCCAGTAAGGGCGAGGGCACTCCTCATACTTACTGCCGGCTGCCCCGC AACCTCAACCCTGGCATGTGGGGCCCAGCAGTGTACCTGTGCTATAAGGTGGGCCTGGCGAAGGCCAAC ACGCTGGTGTACGAGGCAGAGCTGCTGGGCCGCTACCCGGAGGAGGACAATGAGGCGTTCCCGCTGCCC GAGTCAGTGCCCGTCTTCTGCCTGCCCATGGGGGCCACTATCGAGTGCTGGCCTGCCCAGACCAAGTAC CCCGTGCCCGTCTTCTCCACCTTTGTGCTCACGGGTGCAGCTGGTGATAAGGTGTATGGTGCCGCCCTG CAGTTCTACGAGGCGTTCCCAAGGGCCAGGCTATCAGAGCGACAGGCACGGGCACTGGGCCTGCTGAGC GCCGTGGAGCGGGGTCGGGCACTGGGGGGCAGAGCTGTGCGCAGCCGGCGTGCCATCGCTGTGCTGTCC CGCTGGCCTGCCTTCCCTGCCTTCCGCGCCTTCCTCACCTTCCTTTACCGCTACTCCGTCTCAGGCCCC CACCGCCTACCCTTGGAAGCGCACATCTCCCACTTCATTCACAACGTTCCCTTCCCTTCCCCACAGAGA CCCCGCATCCTAGTGCAGATGTCTCCCTATGACAACTTGCTCCTCTGTCAGCCTGTATCCTCACCCCTG CCCCTCAGTGGTGCCAGCTTCCTGCAGCTGCTGCAGAGCCTGGGCCCTGAGCTGGCTATCACACTGCTG CTGGCTGTGCTCACAGAGCACAAGCTGCTAGTCCACTCGCTGCGGCCAGACCTGCTCACCAGCGTCTGT GAGGCCCTCGTCTCGATGATCTTCCCACTGCACTGGCAGTGCCCCTACATTCCTCTGTGCCCGCTGGTG CTGGCAGATGTGCTGAGTGCCCCAGTGCCCTTCATTGTGGGTATCCACTCCAGCTACTTTGATCTGCAT GACCCGCCTGCTGATGTCATCTGTGTAGACCTTGATACCAACACGCTCTTCCAGACTGAGGAAAAGAAG CTCCTCTCCCCTCGGACCCTGCCCCGCAGACCCTACAAGGTTCTGCTGGCCACACTGACAAACCTGTAC CAGCAGCTGGACCAGACATACACTGGACCTGAGGAGGAAGCATCCCTGGAGTTCCTACTGACAGACTAC GAGGCAGTGTGTGGCCGCAGGGCCCGGCTGGAGCGCGAAGTCCAAGGAGCCTTCCTCCGCTTCATGGCC TGTCTGCTCAAGGGCTACCGGGTCTTCCTGCGCCCACTCACCCAGGCCCCCTCCGAGGGAGCTCGTGAT GTTGACAACCTTTTCTTCCTGCAGGGCTTCCTCAAATCCCGGGAACGCTCCAGCCACAAACTTTACTCT CAGCTGCTGCACACACAGATGTTCTCACAGTTCATTGAGGAGTGCTCTTTTGGCTCTGCTCGCCATGCT GCCCTTGAATTCTTTGACTCTTGTGTTGAAAAGGTCCACCCAGAGCAGGAGAAGCCTGAGCCGACACCC TTAGTGGAGCTAGAGGAGCTGTCAGGAAGTGAGCTCACTGTCTTTATCACACCTCCCGAGGAGCCTGCC TTACCAGAGGGCAGTGAATCCACTCCCCAGTACTGCTATGATGGATTCCCAGAGCTACGGGCTGAGTTG TTTGAGTCTCTTCAAGAGCAACCTGGGGCCCTGCCTGTGCCAGGCCCTTCCCGTAGCGCCCCCAGCAGT CCTGCTCCTCGCCGTACCAAACAGGAGATGAAAGTTGCACAGCGGATGGCACAGAAGTCAGCAGCTGTG CCTGAGCTGTGGGCCCGGTGCCTGCTGGGGCACTGCTATGGGCTGTGGTTCCTGTGTCTGCCTGCCTAT GTGCGGTCGGCACCCTCCCGAGTGCAGGCACTGCACACAGCCTACCATGTGCTGCGCCAGATGGAGAGC GGCAAGGTGGTGCTCCCTGATGAGGTGTGTTACCGGGTACTGATGCAGCTCTGCTCACACTATGGGCAG CCTGTGCTGTCTGTGCGGGTCATGCTGGAGATGCGTCAGGCAGGCATTGTGCCCAACACCATCACCTAT GGCTACTACAATAAGGCTGTGTTGGAAAGCAAGTGGCCGTCTGGCACACCAGGTGGGCGTCTGCGCTGG GCCAAGCTCCGGAATGTTGTCCTGGGGGCTGCTCAGTTCCGCCAGCCCTTGAGAGAACGGCAACAGCAG CAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCAGGAGCAGGTGTCAGCACATCAAGAGGCAGGC AGCTCCCAGGCAGAGCCCTATTTGGAGCGCCCTTCCCCTACTCGCCCTCTTCAGCGCCAGACTACTTGG GCTGGGCGAAGTCTGAGAGACCCAGCCTCACCCCCTGGACGCCTGGTGAAGAGTGGTAGCCTGGGCAGT GCCCGAGGGGCACAGCCCACTGTGGAGGCCGGTGTGGCCCACATGATAGAGGCCTTGGGGGTCCTGGAA CCCCGGGGATCACCTGTGCCCTGGCACGATGGAAGTCTCTCAGACCTGAGCCTGACAGGGGAGGAGCCG CTCCCTGGAGGCAGCCCAGGGGGCTCAGGCTCAGCCCTGAGTGCCCAGTCCACTGAGGCCCTGGAAGGG CTAAGTGGGCGGGGACCCAAGGCTGGTGGGCGACAGGATGAGGCAGGCACCCCCCGACGAGGGCTGGGT GCCCGCCTCCAACAGCTGCTCACTCCTTCCCGCCACTCCCCTGCCTCCCGCATTCCCCCACCTGAGCTG CCTCCTGACCTGCCACCCCCAGCCCGCCGCAGCCCCATGGACAGTCTTCTGCACCCCCGGGAGCGCCCT GGATCCACTGCCTCCGAGAGCTCAGCCTCTCTGGGCAGTGAGTGGGACCTCTCAGAATCTTCTCTCAGC AACCTGAGTCTTCGCCGTTCCTCAGAGCGCCTCAGTGACACCCCTGGATCCTTCCAGTCACCTTCCCTG GAAATTCTGCTGTCCAGCTGCTCCCTGTGCCGTGCCTGTGATTCGCTGGTGTATGATGAGGAAATCATG GCTGGCTGGGCACCTGATGACTCTAACCTCAACACAACCTGCCCCTTCTGCGCCTGCCCCTTTGTGCCC CTGCTCAGTGTCCAGACCCTTGATTCCCGGCCCAGTGTCCCCAGCCCCAAATCTGCTGGTGCCAGTGGC AGCAAAGATGCTCCTGTCCCTGGTGGTCCTGGCCCTGTGCTCAGTGACCGAAGGCTCTGCCTTGCTCTG GATGAGCCCCAGCTCTGCAACGGGCACATGGGGGGAGCCTCCCGGCGGGTTGAGAGTGGGGCATGGGCA TACCTGAGCCCCCTGGTGCTGCGTAAGGAGCTGGAGTCGCTGGTAGAGAACGAGGGCAGTGAGGTGCTG GCGTTGCCTGAACTGCCCTCTGCCCACCCCATCATCTTCTGGAACCTTTTGTGGTATTTCCAACGGCTA CGCCTGCCCAGTATTCTACCAGGCCTGGTGCTGGCCTCCTGTGATGGGCCTTCGCACTCCCAGGCCCCA TCTCCTTGGCTAACCCCTGATCCAGCCTCTGTTCAGGTACGGCTGCTGTGGGATGTACTGACCCCTGAC CCCAATAGCTGCCCACCTCTCTATGTGCTCTGGAGGGTCCACAGCCAGATCCCCCAGCGGGTGGTATGG CCAGGCCCTGTACCTGCATCCCTTAGTTTGGCACTGTTGGAGTCAGTGCTGCGCCATGTTGGACTCAAT GAAGTGCACAAGGCTGTGGGGCTCCTGCTGGAAACTCTAGGGCCCCCACCCACTGGCCTGCACCTGCAG AGGGGAATCTACCGTGAGATATTATTCCTGACAATGGCTGCTCTGGGCAAGGACCACGTGGACATAGTG GCCTTCGATAAGAAGTACAAGTCTGCCTTTAACAAGCTGGCCAGCAGCATGGGCAAGGAGGAGCTGAGG CACCGGCGGGCGCAGATGCCCACTCCCAAGGCCATTGACTGCCGAAAATGTTTTGGAGCACCTCCAGAA TGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_014856 |
| Insert Size | 4491 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014856.2 |
| RefSeq Size | 5688 bp |
| RefSeq ORF | 4491 bp |
| Locus ID | 9909 |
| UniProt ID | O75064 |
| Domains | DENN, PPR, dDENN |
| MW | 163.8 kDa |
| Gene Summary | Guanine nucleotide exchange factor (GEF) which may activate RAB10. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form.[UniProtKB/Swiss-Prot Function] |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC220670 | DENND4B (Myc-DDK-tagged)-Human DENN/MADD domain containing 4B (DENND4B) |
CNY 10240.00 |
|
| RC220670L3 | Lenti ORF clone of Human DENN/MADD domain containing 4B (DENND4B), Myc-DDK-tagged |
CNY 16820.00 |
|
| RC220670L4 | Lenti ORF clone of Human DENN/MADD domain containing 4B (DENND4B), mGFP tagged |
CNY 16820.00 |
|
| RG220670 | DENND4B (tGFP-tagged) - Human DENN/MADD domain containing 4B (DENND4B) |
CNY 16440.00 |
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