BAZ2A (NM_013449) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	TIP5; WALp3
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC318689 representing NM_013449. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGAAATGGAGGCAAACGACCATTTTAACTTTACTGGCCTTCCCCCTGCACCTGCTGCCTCAGGACTG AAACCCTCTCCTTCCTCAGGGGAGGGCCTCTACACTAACGGGTCTCCCATGAACTTCCCCCAGCAAGGG AAAAGTTTGAATGGGGATGTGAATGTTAATGGCTTATCTACTGTATCTCACACTACTACTTCAGGGATT TTGAACTCTGCTCCCCACTCCTCCAGCACCTCACACCTCCATCACCCCAGCGTGGCCTACGACTGTCTC TGGAACTACTCACAGTACCCATCTGCCAATCCTGGCAGCAACCTCAAGGACCCACCCCTTCTCTCCCAG TTCTCGGGGGGACAATACCCACTCAACGGCATCCTTGGGGGCAGCCGGCAACCTTCATCCCCAAGTCAT AACACTAACCTTCGGGCTGGGAGCCAAGAGTTCTGGGCCAACGGTACCCAGAGTCCCATGGGGCTTAAC TTTGATTCACAAGAACTGTATGATTCCTTTCCTGACCAGAATTTTGAGGTGATGCCCAATGGACCCCCT AGTTTTTTCACCTCCCCACAGACTTCTCCTATGTTGGGATCTAGCATTCAAACCTTTGCACCCTCCCAG GAGGTAGGCAGTGGTATCCATCCTGATGAGGCAGCAGAAAAGGAGATGACTTCAGTTGTGGCAGAGAAT GGCACTGGCTTGGTAGGCAGCTTGGAGCTGGAAGAAGAGCAGCCAGAACTGAAGATGTGTGGCTACAAT GGCTCTGTCCCTTCTGTGGAATCGTTACACCAAGAGGTCTCAGTCCTGGTCCCTGACCCCACAGTGAGC TGTTTAGATGATCCTTCACATCTTCCTGATCAACTGGAAGACACTCCAATCCTCAGTGAAGACTCTCTG GAGCCCTTCAACTCTCTGGCACCAGAGCCAGTGAGTGGAGGACTATATGGTATTGATGACACGGAGCTG ATGGGTGCAGAGGACAAGCTGCCTCTTGAGGACAGCCCTGTGATTTCTGCCCTTGATTGCCCTTCCCTC AATAATGCTACTGCCTTCAGTCTCCTGGCAGATGATAGTCAAACATCAACCTCTATCTTTGCCAGTCCC ACCTCTCCACCTGTCCTAGGGGAGTCTGTCCTGCAAGATAACAGCTTTGACCTGAATAATGGTAGTGAC GCTGAACAGGAAGAAATGGAAACTCAATCTTCAGACTTCCCACCATCCCTGACCCAGCCAGCTCCTGAT CAGTCATCCACTATTCAGCTACATCCAGCAACCTCACCAGCAGTCTCGCCAACAACCTCCCCAGCAGTC TCCCTAGTGGTTTCTCCAGCAGCCTCCCCAGAAATCTCTCCAGAAGTTTGTCCCGCAGCTTCTACAGTT GTCTCTCCAGCAGTCTTCTCAGTGGTCTCTCCAGCTTCCTCAGCAGTCCTCCCAGCAGTCTCCTTAGAA GTCCCGTTGACGGCTTCAGTGACATCCCCAAAAGCCTCTCCCGTAACTTCCCCAGCAGCTGCCTTTCCA ACAGCCTCCCCAGCAAATAAGGATGTCAGCAGCTTTCTAGAAACCACTGCTGACGTGGAAGAGATCACT GGAGAAGGACTCACTGCTTCTGGTAGTGGTGATGTCATGAGGAGACGTATTGCTACCCCAGAAGAAGTT CGTCTTCCCCTCCAACATGGGTGGCGGAGAGAGGTGCGCATCAAGAAGGGCAGCCACCGATGGCAGGGG GAGACCTGGTATTATGGCCCCTGTGGGAAGAGGATGAAGCAATTTCCAGAAGTGATCAAGTACCTGAGC CGCAACGTGGTACACAGTGTCCGCCGAGAGCACTTCAGCTTCAGTCCCCGTATGCCTGTTGGAGATTTC TTTGAAGAAAGAGACACGCCAGAGGGCTTGCAGTGGGTGCAGCTCTCAGCAGAGGAGATCCCGTCGAGG ATTCAGGCAATTACTGGCAAACGGGGTCGACCTCGAAACACTGAGAAGGCTAAGACTAAGGAAGTCCCC AAGGTGAAACGGGGTCGAGGTCGGCCACCTAAGGTCAAAATCACTGAGCTATTGAACAAGACAGACAAC CGCCCCCTAAAGAAACTGGAGGCCCAAGAAACATTGAATGAGGAGGATAAAGCAAAGATTGCTAAAAGC AAGAAGAAGATGAGGCAGAAGGTTCAACGGGGAGAGTGTCAGACTACTATCCAAGGGCAGGCCAGAAAT AAGCGGAAACAAGAGACCAAGAGCTTAAAGCAGAAGGAAGCTAAGAAGAAATCCAAGGCTGAGAAAGAA AAAGGAAAGACAAAGCAGGAAAAACTGAAGGAAAAAGTCAAGAGGGAAAAGAAGGAGAAGGTAAAAATG AAGGAAAAGGAGGAGGTGACCAAAGCCAAGCCAGCCTGTAAAGCAGATAAGACCCTGGCCACACAGAGG CGCTTGGAGGAACGGCAGAGGCAGCAGATGATCTTGGAGGAAATGAAGAAGCCGACAGAGGATATGTGT CTGACTGACCACCAGCCCCTGCCTGACTTCTCACGAGTCCCTGGTCTGACATTGCCCAGTGGAGCCTTC TCAGACTGCTTGACCATTGTGGAGTTCCTGCATAGCTTTGGCAAGGTGCTGGGCTTTGATCCTGCCAAA GATGTGCCTAGCCTGGGGGTCCTGCAGGAGGGACTCCTGTGTCAAGGTGACAGCTTGGGTGAGGTGCAA GACCTGCTGGTCAGGCTGCTGAAGGCTGCACTCCATGATCCTGGCTTTCCCTCCTACTGTCAGTCCCTA AAGATCTTGGGGGAGAAGGTGTCTGAGATCCCACTGACAAGAGACAATGTGTCAGAGATCCTGCGCTGC TTCCTTATGGCATATGGAGTAGAGCCAGCCCTCTGTGACCGCCTGCGCACCCAGCCTTTTCAGGCCCAG CCACCCCAGCAGAAGGCTGCTGTCCTGGCCTTCCTTGTGCATGAGCTCAATGGCTCCACCCTCATCATC AATGAGATTGACAAGACTCTGGAGAGTATGTCCAGCTACAGGAAAAACAAGTGGATTGTTGAAGGCCGG CTCCGGAGGCTGAAAACTGTTCTGGCCAAGCGAACTGGGCGGTCTGAAGTAGAGATGGAAGGGCCAGAG GAATGCCTGGGACGGAGGCGCAGTTCTCGGATCATGGAGGAGACCAGTGGCATGGAAGAAGAGGAAGAA GAGGAGTCTATAGCAGCTGTCCCTGGCCGCAGGGGTCGAAGAGATGGAGAGGTTGATGCCACAGCATCT AGCATCCCAGAGCTAGAGCGCCAGATAGAAAAACTCAGCAAGCGTCAGCTTTTCTTTCGCAAAAAGCTG CTTCACTCATCCCAGATGCTTCGGGCGGTCTCCCTGGGTCAGGACCGCTACAGACGTCGCTACTGGGTA TTGCCGTATTTGGCTGGTATCTTTGTAGAAGGAACAGAGGGGAACTTAGTTCCTGAGGAGGTGATAAAG AAGGAAACTGACTCCTTAAAAGTGGCAGCCCATGCGTCACTCAACCCTGCCCTCTTCTCTATGAAGATG GAGTTAGCTGGCTCCAACACCACTGCCAGTTCTCCTGCCCGGGCCCGAGGCCGACCTCGAAAAACTAAG CCCGGGTCTATGCAACCTAGGCATCTTAAGTCCCCTGTCAGGGGTCAGGATTCAGAACAGCCCCAGGCC CAGCTTCAGCCTGAGGCTCAGCTTCATGCTCCTGCCCAGCCCCAGCCTCAGCTTCAGCTTCAGCTTCAG TCCCATAAGGGGTTCCTGGAGCAAGAAGGCTCCCCTTTGTCACTGGGTCAGAGCCAGCATGACCTCAGC CAGTCAGCCTTCCTGTCTTGGCTGAGCCAGACTCAGAGCCATAGCTCCCTGTTGAGCAGCTCAGTCCTC ACACCTGATAGCAGTCCGGGAAAACTAGACCCAGCTCCATCACAACCCCCGGAGGAGCCAGAGCCTGAT GAGGCAGAATCCAGCCCTGATCCTCAAGCACTCTGGTTTAACATCTCAGCCCAGATGCCCTGCAATGCT GCCCCCACACCGCCCCCTGCAGTTTCTGAGGACCAACCCACTCCCTCCCCTCAGCAGCTTGCCTCCTCC AAGCCAATGAATAGACCTAGTGCTGCCAACCCTTGTTCTCCAGTGCAGTTCTCTTCCACGCCCTTGGCT GGGTTGGCCCCTAAGAGGCGAGCAGGAGACCCTGGAGAAATGCCACAGAGTCCCACAGGGCTGGGACAG CCCAAACGGAGAGGGAGACCTCCCAGTAAGTTCTTCAAACAGATGGAACAGCGTTACCTAACCCAGCTG ACAGCCCAGCCTGTCCCACCTGAGATGTGCTCAGGCTGGTGGTGGATACGAGATCCTGAGATGTTGGAT GCCATGCTCAAGGCCCTACACCCCCGAGGTATCCGGGAGAAGGCACTTCACAAACACCTTAACAAGCAC AGGGACTTCTTGCAGGAAGTCTGCCTGCGGCCCTCAGCTGACCCCATCTTTGAGCCCAGGCAACTACCT GCCTTTCAAGAAGGGATTATGAGCTGGTCCCCCAAAGAGAAGACATACGAGACAGACCTAGCAGTGCTT CAATGGGTAGAGGAGCTGGAGCAGCGGGTTATCATGTCTGATCTGCAGATTCGGGGCTGGACATGTCCT AGCCCAGACTCTACCCGTGAAGACTTGGCCTACTGTGAGCACCTCTCCGACTCCCAGGAGGATATCACC TGGCGAGGTCGGGGCAGGGAGGGACTGGCACCTCAGCGTAAAACTACCAACCCTTTGGACCTGGCTGTG ATGCGGCTGGCTGCCCTGGAACAGAATGTAGAACGGCGGTACCTGCGGGAGCCCCTCTGGCCAACTCAT GAGGTTGTGCTGGAGAAGGCCCTGCTTAGCACACCTAATGGTGCCCCTGAGGGCACCACTACAGAGATA TCATATGAGATCACCCCTCGCATTCGTGTCTGGCGCCAGACCCTCGAGCGGTGCCGGAGCGCAGCCCAG GTGTGCTTGTGCCTGGGCCAGCTGGAGAGGTCCATTGCCTGGGAGAAGTCTGTCAACAAAGTGACATGT CTAGTCTGCCGGAAGGGTGACAATGATGAGTTTCTTCTGCTTTGTGATGGGTGTGACCGTGGCTGCCAC ATTTACTGCCATCGTCCCAAGATGGAGGCTGTCCCAGAAGGAGATTGGTTCTGTACTGTCTGTTTGGCT CAGCAGGTGGAGGGAGAATTCACTCAGAAGCCTGGTTTCCCAAAGCGTGGCCAGAAGCGGAAAAGTGGT TATTCGCTGAACTTCTCAGAGGGTGATGGCCGCCGACGCCGGGTACTGTTGAGGGGCCGAGAAAGCCCA GCAGCAGGGCCTCGGTACTCGGAAGAAGGGCTCTCCCCCTCCAAGCGGCGGCGACTCTCTATGCGGAAC CACCACAGTGATCTCACATTTTGCGAGATTATCCTGATGGAGATGGAGTCCCATGATGCAGCCTGGCCT TTCCTAGAGCCTGTGAACCCACGTTTGGTGAGTGGGTACCGGCGCATCATCAAAAATCCTATGGATTTT TCCACCATGCGGGAGCGGCTGCTCAGGGGAGGGTACACCAGCTCAGAGGAGTTTGCGGCTGATGCCCTC CTGGTATTTGACAACTGCCAGACTTTCAACGAGGATGACTCTGAAGTAGGCAAGGCTGGGCACATCATG CGCCGCTTCTTCGAGAGCCGCTGGGAGGAGTTTTATCAGGGAAAACAGGCCAATCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_013449
Insert Size	5718 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_013449.3
RefSeq Size	8934 bp
RefSeq ORF	5718 bp
Locus ID	11176
UniProt ID	Q9UIF9
Domains	AT_hook, BROMO, MBD, PHD, DDT
Protein Families	Druggable Genome
MW	211.2 kDa
Gene Summary	Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. In the complex, it plays a central role by being recruited to rDNA and by targeting chromatin modifying enzymes such as HDAC1, leading to repress RNA polymerase I transcription. Recruited to rDNA via its interaction with TTF1 and its ability to recognize and bind histone H4 acetylated on 'Lys-16' (H4K16ac), leading to deacetylation of H4K5ac, H4K8ac, H4K12ac but not H4K16ac. Specifically binds pRNAs, 150-250 nucleotide RNAs that are complementary in sequence to the rDNA promoter; pRNA-binding is required for heterochromatin formation and rDNA silencing (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longer isoform (1).

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