ADAMTS20 (NM_025003) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ADAM-TS20; ADAMTS-20; GON-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC318693 representing NM_025003. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTGGGTGGCCAAGTGGCTGACTGGGCTGCTCTACCATCTCTCGCTCTTCATCACCAGGTCTTGGGAA GTTGACTTCCACCCCAGGCAAGAAGCCCTGGTGAGGACACTGACCTCCTACGAAGTAGTGATCCCCGAG CGGGTCAATGAGTTTGGAGAAGTGTTCCCTCAGAGCCACCACTTCAGCCGGCAGAAACGCAGCTCCGAG GCGCTGGAACCCATGCCGTTCCGAACCCACTATCGCTTCACTGCCTACGGGCAGCTCTTCCAGCTGAAC CTGACCGCCGATGCATCCTTTCTGGCCGCCGGCTACACCGAGGTGCACTTGGGAACCCCGGAGCGCGGG GCCTGGGAGAGCGACGCAGGGCCCTCGGACCTGCGCCACTGCTTCTACCGCGGCCAGGTCAACTCACAG GAGGATTACAAGGCCGTCGTCAGCTTATGCGGAGGCCTGACGGGAACATTTAAAGGACAGAACGGTGAA TATTTCTTAGAACCTATAATGAAGGCAGATGGGAATGAATATGAAGATGGTCACAACAAGCCACATCTT ATATACAGACAAGACTTAAATAACTCTTTTCTGCAGACTCTGAAGTATTGCAGTGTGTCAGAAAGTCAA ATAAAGGAAACCAGTTTACCCTTTCATACCTACAGCAACATGAATGAAGATCTTAATGTAATGAAAGAA AGAGTTTTAGGACACACATCAAAAAATGTACCATTGAAAGATGAAAGAAGACATTCCAGGAAAAAACGT CTTATATCATATCCAAGATACATTGAAATTATGGTTACAGCTGATGCTAAAGTGGTTTCTGCTCATGGA TCGAATTTGCAAAACTATATACTGACTCTAATGTCAATTGTTGCAACAATCTACAAAGATCCAAGTATT GGAAATTTGATACACATAGTAGTGGTAAAATTAGTTATGATTCACCGTGAGGAGGAAGGACCAGTCATT AATTTTGATGGTGCTACCACATTAAAGAACTTTTGTTCATGGCAACAAACTCAGAATGACCTTGATGAT GTTCACCCTTCCCACCATGACACTGCTGTTCTTATCACTAGGGAAGACATTTGTTCATCTAAAGAGAAA TGTAACATGTTAGGTTTATCATATTTAGGTACCATATGTGATCCTTTACAAAGCTGCTTTATTAATGAA GAAAAAGGACTCATTTCTGCTTTTACTATAGCCCATGAGCTTGGGCACACACTTGGTGTTCAACATGAT GATAATCCTAGATGTAAAGAAATGAAAGTTACAAAGTATCATGTAATGGCCCCTGCTTTAAGTTTTCAC ATGAGTCCTTGGAGCTGGTCAAACTGTAGTCGGAAATATGTTACTGAATTCCTAGATACTGGTTACGGG GAATGTCTTCTTGACAAACCAGATGAAGAAATATATAATCTGCCTTCAGAACTTCCTGGATCACGATAT GATGGAAACAAGCAGTGTGAGCTTGCGTTTGGTCCTGGGTCACAAATGTGTCCCCATATAAATATATGC ATGCATCTGTGGTGCACAAGCACAGAAAAGCTTCACAAAGGCTGTTTCACTCAACACGTGCCACCAGCA GATGGAACAGACTGCGGTCCTGGAATGCATTGCCGTCATGGGCTATGTGTAAACAAAGAAACGGAAACA CGTCCTGTAAATGGTGAATGGGGACCATGGGAACCTTACAGTTCTTGTTCAAGAACATGTGGAGGCGGA ATCGAAAGTGCAACCAGGCGCTGTAATCGTCCTGAGCCAAGAAACGGAGGAAATTACTGTGTGGGCCGC AGGATGAAATTTCGATCATGTAATACTGATTCATGTCCAAAAGGCACACAAGACTTTCGAGAGAAGCAG TGCTCTGATTTTAATGGTAAACATTTGGACATCAGTGGCATTCCCTCTAATGTGAGGTGGCTTCCAAGA TACAGTGGCATTGGCACAAAGGATCGTTGTAAACTCTATTGTCAGGTTGCTGGAACCAATTATTTCTAC CTATTGAAGGATATGGTTGAAGATGGTACTCCTTGTGGAACTGAAACTCATGACATCTGTGTTCAAGGC CAGTGTATGGCAGCTGGTTGTGATCACGTGTTAAACTCCAGTGCCAAGATAGACAAATGTGGAGTGTGT GGTGGGGACAACTCTTCATGCAAGACAATAACAGGTGTCTTCAACAGTTCTCATTATGGTTATAATGTT GTTGTAAAGATTCCCGCAGGAGCAACAAACGTTGACATTCGTCAGTACAGCTATTCTGGACAACCAGAT GACAGTTACCTTGCATTATCTGACGCTGAAGGGAATTTTCTTTTCAATGGAAATTTTCTTCTAAGTACG TCAAAAAAAGAAATCAATGTGCAAGGAACAAGAACTGTTATTGAATACAGTGGATCAAATAACGCAGTT GAAAGAATTAATAGTACTAATCGACAAGAGAAAGAACTTATTTTGCAGGTGTTGTGTGTGGGTAATTTA TACAACCCTGATGTACATTATTCCTTCAATATCCCTTTGGAAGAGAGGAGTGACATGTTCACATGGGAC CCCTATGGACCATGGGAAGGCTGTACCAAAATGTGTCAAGGTCTTCAGCGAAGAAACATAACTTGCATA CATAAGAGTGATCATAGTGTTGTGTCTGATAAAGAATGTGACCACTTGCCACTTCCATCATTTGTTACT CAAAGTTGCAATACAGACTGTGAACTAAGGTGGCATGTTATTGGCAAAAGTGAATGTTCATCCCAATGT GGTCAAGGATATAGAACCTTGGACATCCATTGCATGAAGTATTCCATTCATGAAGGACAGACTGTTCAA GTTGATGACCACTACTGTGGTGACCAGCTTAAACCTCCTACCCAAGAACTATGCCATGGTAACTGTGTC TTCACAAGATGGCATTATTCAGAATGGTCTCAGTGTTCCAGGAGTTGTGGAGGAGGGGAAAGGTCTCGA GAATCTTATTGTATGAATAACTTTGGCCATCGTCTTGCTGACAATGAATGCCAAGAACTGTCCCGAGTG ACGAGAGAGAATTGCAATGAATTTTCCTGTCCCAGTTGGGCTGCTAGTGAATGGAGCGAGTGCCTTGTT ACATGTGGTAAAGGAACAAAGCAGCGGCAGGTATGGTGTCAGCTGAATGTAGATCACTTGAGTGATGGC TTCTGTAATTCAAGTACCAAACCTGAATCTCTGAGTCCATGTGAACTTCATACATGTGCTTCCTGGCAA GTAGGACCATGGGGTCCTTGCACAACCACATGTGGACATGGGTATCAGATGCGAGATGTTAAATGTGTC AATGAGCTAGCTAGTGCAGTGTTAGAGGACACAGAATGCCATGAAGCTAGTCGCCCCAGTGACAGACAG AGCTGTGTACTTACACCTTGCTCATTTATTTCTAAACTTGAGACCGCTTTATTACCAACTGTTCTCATA AAAAAGATGGCACAATGGCGACATGGTTCTTGGACCCCATGCTCCGTATCTTGTGGAAGAGGTACTCAA GCCCGCTATGTAAGCTGTCGTGATGCTCTTGATAGAATAGCAGATGAATCATATTGTGCCCACTTACCC CGACCTGCTGAAATATGGGACTGTTTTACCCCTTGTGGAGAGTGGCAAGCAGGGGATTGGTCACCCTGT TCAGCTTCCTGTGGCCATGGAAAAACAACTCGACAAGTTTTATGCATGAACTACCATCAGCCAATTGAT GAGAATTACTGTGATCCTGAAGTTCGCCCTTTGATGGAACAGGAATGTAGCCTGGCAGCCTGCCCTCCT GCACACAGCCACTTTCCTAGTTCCCCTGTGCAGCCAAGCTATTATCTAAGCACGAATTTGCCATTAACT CAAAAACTTGAAGATAATGAAAATCAGGTGGTCCATCCATCAGTCAGAGGAAACCAGTGGAGAACCGGA CCATGGGGATCATGCTCCAGCAGTTGTTCTGGAGGTCTTCAGCATAGGGCTGTGGTCTGCCAGGATGAA AATGGACAAAGTGCTAGTTACTGCGATGCAGCCTCCAAGCCTCCAGAGTTACAGCAATGTGGTCCAGGG CCTTGTCCACAGTGGAACTACGGAAATTGGGGAGAATGTTCACAAACATGTGGAGGAGGAATAAAATCA AGACTTGTAATATGTCAATTTCCCAATGGCCAAATATTAGAAGATCACAACTGTGAAATTGTAAACAAG CCACCTAGCGTAATACAGTGTCATATGCATGCTTGCCCTGCTGATGTGTCATGGCATCAGGAACCATGG ACATCGTGCTCAGCTTCTTGTGGTAAAGGTAGAAAGTACCGTGAAGTGTTTTGCATTGACCAATTCCAA AGGAAATTAGAAGACACAAATTGCAGTCAAGTACAGAAACCTCCAACTCACAAAGCCTGTAGATCTGTC AGATGCCCTTCATGGAAAGCCAATAGCTGGAATGAGTGCTCTGTGACCTGTGGCTCTGGAGTTCAGCAG AGGGATGTATACTGCAGACTGAAAGGTGTTGGTCAGGTGGTTGAAGAAATGTGTGATCAGTCCACCCGA CCTTGTTCTCAGAGGCGATGTTGGAGTCAGGACTGTGTGCAGCACAAGGGGATGGAAAGAGGGAGGCTG AATTGTTCAACATCATGTGAGAGAAAAGATTCACATCAACGAATGGAGTGCACAGATAACCAAATCAGA CAAGTGAATGAAATAGTCTATAATTCTTCAACCATATCTCTTACATCCAAGAATTGCAGGAACCCTCCT TGCAATTACATTGTGGTAACAGCAGACTCATCACAGTGTGCAAACAACTGTGGATTTAGTTACAGACAA AGGATTACATATTGCACCGAGATCCCATCTACTAAGAAACATAAGCTCCATCGACTTCGGCCTATAGTT TATCAAGAATGCCCTGTGGTGCCTTCCTCTCAGGTTTACCAATGCATTAACAGCTGTTTGCATTTGGCC ACTTGGAAAGTTGGAAAATGGAGCAAGTGCTCAGTGACTTGTGGAATTGGGATAATGAAGAGACAAGTG AAATGCATTACCAAACATGGTTTGTCCAGTGACTTATGTTTAAACCATTTAAAACCAGGTGCTCAAAAG AAATGTTATGCCAATGACTGTAAATCATTTACCACCTGCAAAGAAATTCAAGTGAAAAACCACATTAGA AAGGATGGTGACTATTACCTTAACATTAAGGGAAGAATAATAAAGATTTATTGTGCAGACATGTACTTG GAGAACCCTAAGGAATATTTAACACTGGTCCAAGGTGAAGAAAACTTTTCTGAAGTGTATGGCTTTAGA CTAAAAAATCCATATCAATGTCCTTTTAATGGGAGTAGAAGGGAAGACTGTGAATGTGACAATGGACAC TTAGCTGCTGGATACACTGTTTTCAGCAAAATAAGAATTGATCTCACTTCCATGCAAATTAAAACTACG GACCTTCTTTTTTCCAAAACAATATTTGGAAATGCAGTTCCATTTGCCACAGCTGGAGATTGCTACAGT GCTTTCAGATGCCCACAGGGACAGTTTAGCATTAATTTGTCAGGAACTGGGATGAAGATATCCAGCACA GCAAAGTGGCTCACTCAGGGGAGTTATACCTCTGTCAGCATACGAAGATCAGAGGATGGAACTAGATTT TTCGGCAAATGTGGAGGGTACTGTGGAAAGTGTCTTCCTCACATGACTACTGGTCTCCCAATTCAAGTC ATATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_025003
Insert Size	5733 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_025003.3
RefSeq Size	5733 bp
RefSeq ORF	5733 bp
Locus ID	80070
UniProt ID	P59510
MW	214.7 kDa
Gene Summary	The protein encoded by this gene is a member of the ADAMTS family of zinc-dependent proteases. The encoded protein has a signal peptide that is cleaved to release the mature peptide, which is secreted and found in the extracellular matrix. This protein may be involved in tissue remodeling. [provided by RefSeq, Sep 2011]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.