CPAMD8 (NM_015692) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ASGD8; K-CAP; VIP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC318696 representing NM_015692. Blue=Insert sequence Red=Cloning site Green=Tag(s) GTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTA CCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCC ATGGCGCCGGGCGCACTGCGCGGGGGCTGCGAACAAAGGGCCCCCGGCGGCGGCGCGAGGACGGCCGCG CTCGGACCCTGGCCCTGGCCCAGCCCTGGCCCGGCCCCCTCCCCAGGCGCGGCGCCCCCCAGGAGCCGA AAAATGAGCGGCGCCCTGCTCTGGCCGTTGCTCCCGCTCCTGCTCCTGCTGCTGTCGGCGCGGGACGGC GTGCGCGCCGCGCAGCCTCAGGCCCCGGGTTACTTGATTGCAGCTCCCTCTGTTTTTCGCGCGGGCGTG GAGGAAGTCATCAGCGTGACCATCTTTAACTCTCCAAGGGAAGTCACGGTCCAGGCTCAGCTGGTGGCC CAGGGTGAGCCGGTGGTGCAGAGCCAGGGAGCCATCCTGGATAAAGGGACAATCAAACTCAAGGTGCCC ACGGGCCTCCGGGGCCAAGCGCTTCTGAAAGTGTGGGGCCGCGGCTGGCAGGCGGAGGAGGGGCCCCTC TTTCACAACCAGACCTCGGTGACCGTGGACGGCCGGGGCGCTTCTGTATTCATCCAGACGGACAAGCCT GTGTACAGACCCCAGCACCGAGTGCTCATAAGCATCTTCACCGTCTCTCCAAATCTGAGGCCTGTCAAC GAGAAGCTGGAAGCCTACATCCTGGACCCCCGAGGCTCTCGGATGATAGAGTGGAGACACTTAAAGCCG TTCTGCTGCGGCATCACCAACATGAGCTTCCCCTTGTCCGACCAGCCTGTGTTGGGAGAATGGTTCATT TTTGTTGAAATGCAAGGCCACGCGTACAACAAGTCTTTTGAAGTTCAGAAGTATGTGTTGCCCAAGTTT GAGCTTCTGATTGACCCGCCCCGGTATATCCAAGACCTGGACGCCTGTGAGACAGGCACTGTGCGGGCC AGGTATACCTTTGGGAAACCTGTGGCTGGTGCCTTAATGATCAACATGACTGTTAATGGTGTAGGGTAC TACAGCCACGAGGTGGGACGCCCTGTCCTCAGAACAACCAAGATCCTCGGCTCCCGGGACTTCGACATC TGCGTGAGGGACATGATCCCAGCGGACGTCCCTGAGCACTTCCGGGGCAGGGTCAGCATCTGGGCCATG GTGACCAGTGTGGACGGGAGCCAGCAGGTCGCGTTCGATGACTCCACCCCCGTGCAGAGGCAGCTGGTG GACATCCGGTACTCCAAGGACACGAGGAAGCAGTTCAAGCCGGGCCTGGCCTACGTGGGGAAGGTGGAG CTATCCTACCCCGATGGCAGCCCAGCTGAGGGGGTGACGGTCCAGATTAAGGCAGAGCTGACACCAAAG GATAACATCTACACCAGTGAAGTTGTGTCCCAGCGTGGACTAGTGGGGTTTGAAATCCCCTCCATCCCC ACGTCAGCCCAGCACGTGTGGCTGGAGACCAAGGTGATGGCACTGAACGGGAAGCCTGTGGGGGCTCAG TACCTGCCCAGCTACCTCTCCCTCGGCAGCTGGTACTCCCCCAGCCAGTGCTACCTGCAGCTGCAGCCA CCCTCCCACCCACTGCAGGTTGGGGAAGAAGCCTATTTTTCTGTGAAGTCCACATGTCCCTGCAACTTT ACCCTGTACTACGAGGTGGCTGCACGGGGCAATATTGTGCTATCGGGCCAGCAGCCTGCCCACACCACC CAGCAGCGAAGCAAGCGGGCGGCCCCTGCCCTGGAGAAACCGATTCGTTTAACACACCTTTCTGAGACA GAGCCCCCACCAGCCCCAGAAGCTGAGGTCGACGTGTGTGTGACCTCTCTTCATCTGGCCGTGACCCCC AGCATGGTCCCCCTTGGTCGCCTGCTGGTCTTCTACGTCAGGGAGAATGGAGAAGGGGTCGCCGACAGC CTTCAGTTTGCAGTCGAGACCTTCTTCGAAAACCAGGTTTCAGTGACGTATTCAGCAAATGAGACCCAA CCTGGGGAGGTTGTCGACCTGCGGATCAGGGCTGCAAGGGGCAGCTGTGTGTGCGTCGCCGCAGTTGAT AAGAGTGTCTACCTGCTCAGGTCTGGGTTCCGGCTGACTCCTGCCCAGGTTTTCCAGGAACTGGAAGAT TATGATGTTTCTGATTCCTTTGGCGTGTCCAGGGAGGATGGTCCTTTTTGGTGGGCTGGGCTGACGGCA CAACGACGCCGGCGCTCCTCTGTCTTCCCGTGGCCTTGGGGCATCACCAAGGACTCTGGGTTTGCCTTC ACCGAAACGGGACTGGTGGTGATGACCGACCGAGTGAGCCTGAACCACCGGCAGGACGGTGGCCTCTAC ACCGATGAGGCTGTCCCCGCTTTCCAGCCCCACACAGGGAGCCTGGTGGCAGTGGCTCCTTCCAGGCAC CCCCCCAGAACAGAGAAGAGAAAAAGGACTTTCTTCCCCGAAACATGGATTTGGCATTGTCTCAACATC AGTGACCCATCTGGTGAGGGGACACTCAGTGTGAAGGTCCCGGACTCCATCACCAGCTGGGTGGGTGAG GCCGTGGCCCTGTCCACCTCTCAGGGCTTAGGCATCGCCGAGCCCTCCCTGCTGAAGACCTTCAAGCCC TTCTTCGTGGACTTCATGCTCCCCGCTCTCATCATCCGTGGGGAGCAGGTCAAGATCCCGCTCAGTGTC TACAACTACATGGGCACCTGCGCTGAGGTGTACATGAAGCTCTCGGTTCCCAAGGGCATCCAGTTTGTT GGGCATCCTGGCAAACGCCATGTGACCAAGAAGATGTGTGTGGCCCCCGGGGAGGCTGAGCCCATCTGG GTCGTTCTGTCCTTCAGCGACCTGGGACTCAACAACATCACGGCCAAAGCCCTTGCTTACGGAGACACA AATTGCTGCCGGGATGGGAGGTCCAGCAAACACCCTGAGGAGAATCACGCCGACAGGAGGGTCCCCATC GGGGTGGATCACGTCAGGCGCAGTGTGATGGTTGAGGCGGAAGGAGTCCCCCGGGCGTACACCTACAGC GCATTCTTCTGTCCCAGTGAGAGAGTCCACATCTCCACCCCCAACAAGTATGAGTTCCAGTATGTGCAG CGGCCACTGCGCCTCACCCGCTTTGATGTGGCTGTGCGAGCTCACAATGATGCCCGTGTGGCCTTGTCT TCTGGGCCCCAGGACACAGCAGGCATGATCGAGATCGTCCTGGGGGGGCATCAGAACACCAGGTCATGG ATCTCCACCAGCAAGATGGGAGAGCCCGTGGCCAGTGCACACACGGCCAAGATCCTCTCCTGGGATGAA TTCAGAACATTCTGGATCAGCTGGCGTGGTGGCCTTATCCAGGTTGGCCATGGTCCAGAGCCATCCAAT GAGTCTGTCATTGTGGCCTGGACCCTCCCGAGGCCACCAGAGGTCCAGTTCATTGGCTTTTCCACCGGC TGGGGCTCCATGGGTGAATTCCGAATCTGGAGGAAGATGGAGGTGGACGAGAGCTACAGCGAGGCCTTC ACCCTGGGGGTCCCACACGGCGCCATCCCTGGGTCTGAGCGAGCCACCGCCTCCATCATCGGGGACGTC ATGGGGCCAACCCTGAACCACCTCAACAACCTCCTGCGGCTGCCGTTTGGCTGTGGAGAGCAGAACATG ATCCACTTTGCACCCAACGTCTTTGTCTTGAAGTATCTTCAGAAAACCCAGCAGCTCAGCCCTGAGGTG GAGAGAGAGACCACCGACTACCTAGTACAAGGCTACCAGCGCCAGCTGACCTACAAGCGCCAGGATGGC TCCTACAGCGCGTTTGGGGAGCGGGACGCATCGGGGAGCATGTGGCTCACAGCCTTTGTCCTGAAGTCC TTCGCACAGGCTCGCAGCTTTATCTTCGTGGACCCCCGGGAGCTGGCTGCCGCCAAGAGCTGGATCATC CAGCAGCAGCAGGCCGATGGCTCCTTCCTGGCCGTGGGCAGGGTCCTGAACAAGGACATCCAGGGTGGG ATCCACGGCACTGTCCCGCTGACAGCCTACGTGGTGGTTGCTCTCCTGGAAACAGGCACAGCCTCAGAG GAGGAGAGAGGCTCCACTGACAAAGCGAGGCACTTCCTGGAGTCTGCTGCGCCCCTGGCCATGGACCCT TATAGCTGTGCCCTGACTACCTACGCGCTGACCCTGCTCCGCAGCCCGGCAGCCCCTGAGGCACTGCGC AAGCTCCGTAGCCTGGCCATCATGCGAGATGGGGTCACCCACTGGAGCCTGTCAAATTCCTGGGACGTG GACAAGGGCACATTCTTGAGCTTCAGTGACAGGGTCTCTCAGTCAGTGGTCTCGGCCGAGGTGGAAATG ACAGCCTACGCCCTTCTGACCTACACTCTGCTGGGTGACGTGGCTGCCGCCCTGCCTGTGGTGAAGTGG CTGTCCCAGCAGCGAAATGCACTTGGGGGCTTCTCCTCCACTCAGGACACCTGCGTGGCTCTGCAGGCC TTGGCTGAATATGCCATCTTGTCCTATGCTGGAGGCATCAACCTCACTGTCTCCCTGGCCTCCACCAAC CTGGACTACCAGGAAACCTTCGAGCTGCACAGGACCAACCAGAAGGTTCTGCAGACAGCAGCGATCCCC AGCCTCCCCACGGGGCTGTTTGTGAGTGCCAAGGGGGACGGCTGCTGCCTGATGCAGATTGATGTCACC TACAATGTGCCTGACCCGGTGGCCAAGCCAGCTTTCCAGCTGCTCGTAAGCCTCCAGGAGCCTGAGGCC CAGGGACGCCCGCCCCCCATGCCTGCCTCCGCAGCTGAGGGTTCCCGAGGAGACTGGCCCCCAGCTGAC GATGATGACCCAGCGGCCGATCAGCATCACCAGGAATACAAGGTGATGCTGGAGGTGTGCACCAGGTGG CTGCATGCAGGGTCTTCCAATATGGCTGTCCTGGAGGTGCCCCTGCTGTCAGGCTTCCGGGCAGACATC GAGAGCCTGGAGCAGCTGCTCCTTGACAAGCACATGGGGATGAAGAGGTATGAAGTGGCTGGACGCCGA GTGCTCTTCTACTTTGATGAGATCCCCAGCCGGTGCCTGACGTGCGTGCGGTTCCGTGCTCTCCGGGAG TGCGTGGTGGGCAGGACGTCGGCGCTGCCAGTCTCCGTGTACGACTACTACGAACCCGCCTTCGAGGCC ACTCGCTTCTACAACGTCAGCACCCACAGCCCACTCGCCCGGGAACTGTGCGCCGGACCCGCGTGCAAC GAAGTGGAGCGCGCCCCTGCCCGGGGCCCGGGCTGGTTCCCCGGCGAGTCGGGCCCTGCCGTGGCCCCT GAGGAGGGGGCGGCGATCGCGCGATGCGGCTGCGACCACGACTGCGGCGCCCAGGGGAACCCGGTGTGC GGCTCCGACGGGGTGGTCTACGCCAGCGCCTGCCGCCTGCGGGAGGCCGCCTGCCGCCAGGCCGCGCCC CTGGAGCCCGCGCCTCCCAGCTGCTGCGCCCTCGAGCAGCGGCTGCCGGCCTCGTCGTCCTCCACCTAC GGGGATGACCTGGCTTCTGTGGCCCCGGGGCCTTTACAGCAGGACGTGAAGCTGAATGGAGCCGGCCTT GAGGTGGAGGACTCAGACCCTGAGCCTGAAGGGGAGGCGGAGGACAGGGTCACAGCCGGGCCTCGGCCT CCTGTGAGCAGCGGGAACCTGGAAAGCAGCACCCAGAGCGCCAGCCCGTTCCACAGATGGGGCCAGACT CCGGCCCCTCAGAGACATAGTGGCCGGGTGGTGGGGGCCCACAGGCCAGGGCTTCTGAGCCCTGTCTTC GTCTACAGCCCAGCCTTTCAGAGTGGTGGGGAGGAGGGTTTATGGATGTCAAACACCTGCACCTTGAGA TAA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	AscI-RsrII
ACCN	NM_015692
Insert Size	5799 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_015692.2
RefSeq Size	5996 bp
RefSeq ORF	5799 bp
Locus ID	27151
MW	211.3 kDa
Gene Summary	This gene encodes a member of the protease inhibitor I39 (alpha-2-macroglobulin) family of proteins. These proteins are important in innate and acquired immunity. The encoded protein is membrane-associated and proteolytically processed to generate two chains. Mutations in this gene cause a form of anterior segment dysgenesis, a developmental disorder of the eye. [provided by RefSeq, May 2017] Transcript Variant: This variant (1) represents the longer transcript and encodes the protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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