GEFT (ARHGEF25) (NM_001111270) Human Untagged Clone
CAT＃: SC318957
ARHGEF25 (untagged)-Human Rho guanine nucleotide exchange factor (GEF) 25 (ARHGEF25), transcript variant 3
CNY 9980.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | GEFT; p63RhoGEF |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC318957 representing NM_001111270.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAAGCCCCCGGACCGCCCCGCCCCTGGCCGCACTGACCGGATACTGGGGGTCATGGGGGGCATGCTG CGCGCATGCGCCCTCCCTGGGCAGGAGGGGCCCCCAAGGAGAAGCCCTCTAGGGTTGGTGGGTACCGAG CCAGAGTCTGAACGTACGGAGGGAGATCACAGAAGGGATCGCGAACATGAGGTCCTCGCCGGGGCTCTG CAGCCCGAATCCTATTCCATTGCGGGCAGTGAGGGGAGTATATCGGCTTCTGCTGCCTCCGGTCTGGCT GCCCCCTCTGGCCCCAGCTCTGGCCTCAGCTCTGGCCCCTGTTCCCCAGGCCCCCCAGGGCCCGTCAGT GGCCTGAGGAGATGGTTGGATCATTCCAAACATTGTCTCAGTGTGGAAACTGAGGCAGACAGTGGTCAG GCAGGACCATATGAGAACTGGATGTTGGAGCCAGCTCTAGCCACAGGAGAGGAGCTGCCGGAACTGACC TTGCTGACCACACTGTTGGAGGGCCCTGGAGATAAGACGCAGCCACCTGAAGAGGAGACTTTGTCCCAA GCCCCTGAGAGTGAGGAGGAACAGAAGAAGAAGGCTCTGGAAAGGAGTATGTATGTCCTGAGTGAACTG GTAGAAACAGAGAAAATGTACGTGGACGACTTGGGGCAGATTGTGGAGGGTTATATGGCCACCATGGCT GCTCAGGGGGTCCCCGAGAGTCTTCGAGGCCGTGACAGGATTGTGTTTGGGAATATCCAGCAAATCTAT GAGTGGCACCGAGACTATTTCTTGCAAGAGCTACAACGGTGTCTGAAAGATCCTGATTGGCTGGCTCAG CTATTCATCAAACACGAGCGCCGGCTGCATATGTATGTGGTGTACTGTCAGAATAAGCCCAAGTCAGAG CATGTGGTGTCAGAGTTTGGGGACAGCTACTTTGAGGAGCTCCGGCAGCAGCTGGGGCACCGCCTGCAG CTGAACGACCTCCTCATCAAACCTGTGCAGCGGATCATGAAATACCAGCTGCTGCTCAAGGATTTTCTC AAGTATTACAATAGAGCTGGGATGGATACTGCAGACCTAGAGCAAGCTGTGGAGGTCATGTGCTTTGTG CCCAAGCGCTGCAACGATATGATGACGCTGGGGAGATTGCGGGGATTTGAGGGCAAACTGACTGCTCAG GGGAAGCTCTTGGGCCAGGACACTTTCTGGGTCACCGAGCCTGAGGCTGGAGGGCTGCTGTCTTCCCGA GGTCGAGAGAGGCGCGTCTTCCTCTTTGAGCAAATCATCATCTTCAGTGAAGCCCTGGGAGGAGGAGTG AGAGGTGGAACACAGCCTGGATATGTATACAAGAACAGCATTAAGGTGAGCTGCCTGGGACTGGAGGGG AACCTCCAAGGTGACCCTTGCCGCTTTGCACTGACCTCCAGAGGGCCAGAGGGTGGGATCCAGCGCTAT GTCCTGCAGGCTGCAGACCCTGCTATCAGTCAGGCCTGGATCAAGCATGTGGCTCAGATCTTGGAGAGC CAACGGGACTTCCTCAACGCATTGCAGTCACCCATTGAGTACCAGAGACGGGAGAGCCAGACCAACAGC CTGGGGCGGCCAAGAGGGCCTGGAGTGGGGAGCCCTGGAAGAATTCGGCTTGGAGATCAGGCCCAGGGC AGCACACACACACCCATCAATGGCTCTCTCCCCTCTCTGCTGCTGTCACCCAAAGGGGAGGTGGCCAGA GCCCTCTTGCCACTGGATAAACAGGCCCTTGGTGACATCCCCCAGGCTCCCCATGACTCTCCTCCAGTC TCTCCAACTCCAAAAACCCCTCCCTGCCAAGCCAGACTTGCCAAGCTGGATGAAGATGAGCTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001111270 |
| Insert Size | 1860 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001111270.2 |
| RefSeq Size | 2257 bp |
| RefSeq ORF | 1860 bp |
| Locus ID | 115557 |
| UniProt ID | Q86VW2 |
| MW | 68.1 kDa |
| Gene Summary | Rho GTPases alternate between an inactive GDP-bound state and an active GTP-bound state, and GEFs facilitate GDP/GTP exchange. This gene encodes a guanine nucleotide exchange factor (GEF) which interacts with Rho GTPases involved in contraction of vascular smooth muscles, regulation of responses to angiotensin II and lens cell differentiation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (3) differs in the 5' UTR, and in the presence and absence of exons in the 5' coding region, compared to variant 1. The resulting isoform (3) contains a distinct N-terminus and is longer than isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226030 | ARHGEF25 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 25 (ARHGEF25), transcript variant 3 |
CNY 4608.00 |
|
| RC226030L3 | Lenti-ORF clone of ARHGEF25 (Myc-DDK-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 25 (ARHGEF25), transcript variant 3 |
CNY 7320.00 |
|
| RC226030L4 | Lenti-ORF clone of ARHGEF25 (mGFP-tagged)-Human Rho guanine nucleotide exchange factor (GEF) 25 (ARHGEF25), transcript variant 3 |
CNY 7320.00 |
|
| RG226030 | ARHGEF25 (tGFP-tagged) - Human Rho guanine nucleotide exchange factor (GEF) 25 (ARHGEF25), transcript variant 3 |
CNY 5990.00 |
