KLHL22 (NM_032775) Human Untagged Clone
CAT＃: SC319908
KLHL22 (untagged)-Human kelch-like 22 (Drosophila) (KLHL22), transcript variant 1
CNY 4728.00
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | KELCHL |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_032775.2
CCGCCTCGCGCCTCCCGCCTCCCGCCTCCCGCCTCCCTCCAGCTGCGAGTGCGGCCTCGG
CTGGCGGCGGCACCAGGCCACAGTTGTAAGGGATCTTGTGGCTGTCAGGATGGCAGAGGA GCAGGAGTTCACCCAGCTCTGCAAGTTGCCTGCACAGCCCTCACACCCACACTGCGTGAA CAACACCTACCGCAGCGCACAGCACTCCCAGGCTCTGCTCCGAGGGCTGCTGGCTCTCCG GGACAGCGGAATCCTCTTCGATGTTGTGCTGGTGGTGGAGGGCAGACACATCGAGGCCCA TCGCATCCTGCTGGCTGCGTCCTGCGATTACTTCAGAGGAATGTTTGCTGGGGGATTGAA GGAGATGGAACAGGAAGAGGTCCTGATCCACGGTGTGTCCTACAATGCTATGTGCCAAAT CCTACATTTCATATACACCTCCGAGCTGGAGCTCAGCCTGAGCAATGTACAAGAGACACT GGTGGCTGCCTGCCAGCTTCAGATCCCAGAAATTATCCATTTCTGCTGTGATTTCCTCAT GTCCTGGGTGGACGAAGAGAACATTCTCGATGTCTACCGGCTGGCAGAGCTGTTTGACTT GAGCCGCCTGACTGAGCAACTGGACACCTATATCCTCAAAAACTTTGTGGCCTTCTCTCG GACTGACAAGTACCGCCAGCTTCCATTGGAGAAGGTCTACTCCCTCCTCAGCAGCAATCG CCTGGAGGTCTCCTGCGAGACCGAGGTATATGAGGGGGCCCTTCTCTACCATTATAGCCT GGAGCAGGTGCAGGCTGACCAGATCTCGCTGCACGAGCCCCCAAAGCTCCTTGAGACAGT GCGGTTTCCGCTGATGGAAGCTGAGGTCCTGCAGCGGCTGCATGACAAGCTGGACCCCAG CCCTTTGAGGGACACAGTGGCCAGCGCCCTCATGTACCACCGGAACGAGAGCCTACAGCC CAGCCTGCAGAGCCCGCAAACGGAGCTGCGGTCGGACTTCCAGTGCGTTGTGGGCTTCGG GGGCATTCACTCCACGCCGTCCACTGTCCTCAGCGACCAGGCCAAGTATCTAAACCCCTT ACTGGGAGAGTGGAAGCACTTCACTGCCTCCCTGGCCCCCCGCATGTCCAACCAGGGCAT CGCGGTGCTCAACAACTTCGTATACTTGATTGGAGGGGACAACAATGTCCAAGGATTTCG AGCAGAGTCCCGATGCTGGAGGTATGACCCACGGCACAACCGCTGGTTCCAGATCCAGTC CCTGCAGCAGGAGCACGCCGACCTGTCCGTGTGTGTTGTAGGCAGGTACATCTACGCTGT GGCGGGCCGTGACTACCACAATGACCTGAATGCTGTGGAGCGCTACGACCCTGCCACCAA CTCCTGGGCATACGTGGCCCCACTCAAGAGGGAGGTGTATGCCCACGCAGGCGCGACGCT GGAGGGGAAGATGTATATCACCTGCGGCCGCAGAGGGGAGGATTACCTGAAAGAGACACA CTGCTACGATCCAGGCAGCAACACTTGGCACACACTGGCTGATGGGCCTGTGCGGCGCGC CTGGCACGGCATGGCAACCCTCCTCAACAAGCTGTATGTGATCGGGGGCAGCAACAACGA TGCCGGATACAGGAGGGACGTGCACCAGGTGGCCTGCTACAGCTGCACGTCTGGACAGTG GTCATCTGTCTGCCCACTCCCTGCTGGGCACGGTGAGCCTGGCATTGCTGTGCTGGACAA CAGGATCTATGTGTTAGGTGGCCGCTCACACAACCGCGGCAGCCGCACAGGCTACGTGCA CATTTACGATGTGGAGAAGGACTGCTGGGAGGAAGGGCCCCAGCTGGACAACTCCATCTC AGGCCTGGCGGCCTGTGTGCTCACCCTGCCCCGCTCCCTGCTCCTTGAGCCGCCCCGCGG GACCCCTGACCGCAGCCAGGCCGACCCGGACTTTGCCTCTGAGGTGATGAGTGTGTCTGA CTGGGAGGAGTTTGACAACTCCAGTGAGGACTAGGCTCCCTGTGCCTGGCATCAGAGGGA AGGGAGGCTGGGGCTGCAGGGCAGTGAAACCCACGCAGCCTAGGCCAGAGCACTTTACTC CAGGCAGGGCCCCTGCCCCTGTTGGCCACAGCTTATGGGCCCTGCCCTCTTTCTTCACAC CAGCCCCTTGGCCAGCCTGCCGCAGGCATCTCAGGGCCATGCAGATCCCAGGCTGTGGAT TACGTCACTGCAGGAACTGCTTCTGAAAGGCTTTGGCCTTCCTCAGTGAGCCCTGGAGGG GTTGGCCTTCTCTCCTCCCTGCCTAGATCAGTCCTTCTCCAAAAGGACCTGGGAGCTGGC AGCCTGGGCAGGGGGGCTGAGGCACGTGGTAGCCCTCTTTCTGTGAATGGGGTGGGGCTG TGGGGGCAGCTGGGGTTGGAGGATGAGAGCTCCTGAGGGGCAGAGGAGCAGAGATGCATC AGCTCGCCTGGGACTGGCTCTGTGATAAAAAGACCCCTCCCGCTGGGTTTTCAGACCCCA TGTTGTTGGTCAATGAAATAAAGCATCTTACAGAAGGTGTTAACTGTTAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_032775 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_032775.2, NP_116164.2 |
| RefSeq Size | 2602 bp |
| RefSeq ORF | 1905 bp |
| Locus ID | 84861 |
| UniProt ID | Q53GT1 |
| Domains | BTB, Kelch |
| Gene Summary | Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation (PubMed:19995937, PubMed:23455478). The BCR(KLHL22) ubiquitin ligase complex is also responsible for the amino acid-stimulated 'Lys-48' polyubiquitination and proteasomal degradation of DEPDC5. Through the degradation of DEPDC5, releases the GATOR1 complex-mediated inhibition of the TORC1 pathway. It is therefore an amino acid-dependent activator within the amino acid-sensing branch of the TORC1 pathway, indirectly regulating different cellular processes including cell growth and autophagy (PubMed:29769719).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the functional protein. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204296 | KLHL22 (Myc-DDK-tagged)-Human kelch-like 22 (Drosophila) (KLHL22), transcript variant 1 |
CNY 4720.00 |
|
| RC204296L1 | Lenti ORF clone of Human kelch-like 22 (Drosophila) (KLHL22), transcript variant 1, Myc-DDK-tagged |
CNY 7120.00 |
|
| RC204296L2 | Lenti ORF clone of Human kelch-like 22 (Drosophila) (KLHL22), transcript variant 1, mGFP tagged |
CNY 6460.00 |
|
| RC204296L3 | Lenti ORF clone of Human kelch-like 22 (Drosophila) (KLHL22), transcript variant 1, Myc-DDK-tagged |
CNY 6460.00 |
|
| RC204296L4 | Lenti ORF clone of Human kelch-like 22 (Drosophila) (KLHL22), transcript variant 1, mGFP tagged |
CNY 6460.00 |
|
| RG204296 | KLHL22 (tGFP-tagged) - Human kelch-like 22 (Drosophila) (KLHL22), transcript variant 1 |
CNY 6320.00 |
