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Seladin 1 (DHCR24) (NM_014762) Human Untagged Clone
CAT＃: SC320135
DHCR24 (untagged)-Human 24-dehydrocholesterol reductase (DHCR24)
CNY 5,776.00
| Cited in 2 publications. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DCE; Nbla03646; seladin-1; SELADIN1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_014762.3
GCGGAGCTGGCGGCAGTGACAGGAGGCGCGAACCCGCAGCGCTTACCGCGCGGCGCCGCA
CCATGGAGCCCGCCGTGTCGCTGGCCGTGTGCGCGCTGCTCTTCCTGCTGTGGGTGCGCC TGAAGGGGCTGGAGTTCGTGCTCATCCACCAGCGCTGGGTGTTCGTGTGCCTCTTCCTCC TGCCGCTCTCGCTTATCTTCGATATCTACTACTACGTGCGCGCCTGGGTGGTGTTCAAGC TCAGCAGCGCTCCGCGCCTGCACGAGCAGCGCGTGCGGGACATCCAGAAGCAGGTGCGGG AATGGAAGGAGCAGGGTAGCAAGACCTTCATGTGCACGGGGCGCCCTGGCTGGCTCACTG TCTCACTACGTGTCGGGAAGTACAAGAAGACACACAAAAACATCATGATCAACCTGATGG ACATTCTGGAAGTGGACACCAAGAAACAGATTGTCCGTGTGGAGCCCTTGGTGACCATGG GCCAGGTGACTGCCCTGCTGACCTCCATTGGCTGGACTCTCCCCGTGTTGCCTGAGCTTG ATGACCTCACAGTGGGGGGCTTGATCATGGGCACAGGCATCGAGTCATCATCCCACAAGT ACGGCCTGTTCCAACACATCTGCACTGCTTACGAGCTGGTCCTGGCTGATGGCAGCTTTG TGCGATGCACTCCGTCCGAAAACTCAGACCTGTTCTATGCCGTACCCTGGTCCTGTGGGA CGCTGGGTTTCCTGGTGGCCGCTGAGATCCGCATCATCCCTGCCAAGAAGTACGTCAAGC TGCGTTTCGAGCCAGTGCGGGGCCTGGAGGCTATCTGTGCCAAGTTCACCCACGAGTCCC AGCGGCAGGAGAACCACTTCGTGGAAGGGCTGCTCTACTCCCTGGATGAGGCTGTCATTA TGACAGGGGTCATGACAGATGAGGCAGAGCCCAGCAAGCTGAATAGCATTGGCAATTACT ACAAGCCGTGGTTCTTTAAGCATGTGGAGAACTATCTGAAGACAAACCGAGAGGGCCTGG AGTACATTCCCTTGAGACACTACTACCACCGCCACACGCGCAGCATCTTCTGGGAGCTCC AGGACATCATCCCCTTTGGCAACAACCCCATCTTCCGCTACCTCTTTGGCTGGATGGTGC CTCCCAAGATCTCCCTCCTGAAGCTGACCCAGGGTGAGACCCTGCGCAAGCTGTACGAGC AGCACCACGTGGTGCAGGACATGCTGGTGCCCATGAAGTGCCTGCAGCAGGCCCTGCACA CCTTCCAAAACGACATCCACGTCTACCCCATCTGGCTGTGTCCGTTCATCCTGCCCAGCC AGCCAGGCCTAGTGCACCCCAAAGGAAATGAGGCAGAGCTCTACATCGACATTGGAGCAT ATGGGGAGCCGCGTGTGAAACACTTTGAAGCCAGGTCCTGCATGAGGCAGCTGGAGAAGT TTGTCCGCAGCGTGCATGGCTTCCAGATGCTGTATGCCGACTGCTACATGAACCGGGAGG AGTTCTGGGAGATGTTTGATGGCTCCTTGTACCACAAGCTGCGAGAGAAGCTGGGTTGCC AGGACGCCTTCCCCGAGGTGTACGACAAGATCTGCAAGGCCGCCAGGCACTGAGCTGGAG CCCGCCTGGAGAGACAGACACGTGTGAGTGGTCAGGCATCTTCCCTTCACTCAAGCTTGG CTGCTTTCCTAGATCCACACTTTCAAAGAGAAACCCCTCCAGAACTCCCACCCTGACAGC CCAACACCACCTTCCTCCTGGCTTCCAGGGGGCAGCCCAGTGGAATGGAAAGAATGTGGG ATTTGGAGTCAGACAAGCCTGAGTCCAGTTCCCCGTTTAGAACTCATTAGCTGTGTGACT CTGGGTGAGTCCCTTAACCCCTCTGAGCCCGGGTCTCTTCATTAGTTGAAAGGGATAGTA ATACCTACTTGCAGGTTGTTGTCATCTGAGTTGAGCACTGGTCACATTGAAGGTGCTGGG TAAGTGGTAGCTCTTGTTGCTTCCCGTTCAGCGTCACATCTGCAGTGGAGCCTGAAAAGG CTCCACATTAGGTCACCTGTGCACAGCCATGGCTGGAATGATGAAGGGGATACGCTGGAG TTGCCCTGCCATCGCCTCCATCAGCCAGACGAGGTCCTCACAGGAGAAGGACAGCTCTTC CCCACCCTGGGATCTCAGGAGGGCAGCCACGGAGTGGGGAGGCCCCAGATGCGCTGTGCC AAAGCCAGGTCCGAGGCCAAAGTTCTCCCTGCCATCCTTGGTGCCGTCCTGCCCCTTCCT CCTTCATGCCTGGGCCTGCAGGCCCACCCCAGCCACCACTGAGTCCACTCGGAGTGCCCT GTGTTCCTGGAGAAGGCATTCCAGGGTTGAATCTTGTCCCAGCCTCAGCCTGGGACACCT AGGTGGAGAGAGTGGTCTCCGCTCTGAATTGGATCCAGGGGACCTGGGCTCATTCTTCTT GGCTCACCAACCCTGCAGGCCTCATCTTTCCCAAAACCCACTTTGTCTTGGTGGGAGTGG GTCCGCGCTGCTCTGCAGCAGGGGCTGGGGAGTGGGCAGCATCAGGTGGGAAAGTGGAGT CCACCCTCATGTTTCTGTAGGATTCTCACCGTGGGGCTGGAAGAAAAGAGCATCGACTTG ATTTCTCCAACCACTCATCCCTCTTTTTCTTTCTTCCACCACTCCCCACCCCAGCTGTAG TTAATTTCAGTGCCTTACAAATCCTAAGCTCAGAGAAAGTTCCATTTCCGTTCCAGAGGG AAGGGAACCTCCCTAGGTCCTTCCCTGGCTTGTTATAACGCAAAGCTTGGTTGTTTATGC AACTCTATCTTAAGAACTGCCCAGCCTCAGCTGAAAACCCGAATCTGAGAAGGAATTGCG TCATGTAAGGGAAGCTGGAATTAAGGGAGCTGAGCCAGTCATGGTTGTGGCGTGTGAGTC AGGAGACCTAGGTTTCAGCCCCTCTCTACTGTCAGCGAGCTGTGCAACGTGGGCAAGTCA TTGTCCTCTGAGCTGCAGTTTCCTCATCTGTCACATCGCTACAGACAAGACCTCCCTGGA ACCCTTCTGATTGTCTTAGACACTGTGGTTGCAAAACCCACGGAAAGCCTCATTTGTGTG GAAAGTCAGAGGAAAAATGATCCAGTGGACACTTGGGGATTATCTGTCATTCAAGATCCT TCCTTCAACCCCAAGGTCAGCTCCCATCTCATTTCCAGAAAGGCTCATACCTGGCTTGCA GGGAAGCATCTGTCTTGTCATTCCAGGTGCCAGAATCCTCTCAGAGTCATTGAAGGGTGT TCACCCATCCCACCCAAGGCTTGGCACACTGCCAGTGTCTTAGCAGGGTCTTGTGAGGGC TGGGGGCATCCAGGCACTCAGAAGGCAAAGGAACCACCCTACCCATTTGGCCTCTGGAGG GGGCAGAAGAAAGAAAGAAACCTCATCCTATATTTTACAAAGCATGTGAATTCTGGCATT AGCTCTCATAGGAGACCCATGTGCTTCCTTGCTCAGTGCAAAACTGATGATTCTACTTGC TGTAGATGAATGGTTAACACGAGCTAGTTAAACAGTGCCATTGTTTTGCCAGTGAAGCCT CCAACCCTAAGCCACTGGGACGGTGGCCAGAGATGCCAGCAGCCTCTGTCGCCCTTAGTC ATATAACCAAAATCCAGACCTTATCCACAACCCGGGGCTTGGAAAGGAAGGTATTTTGGA ATCACACCCTCCGGTTATGTTGCTCCAGTAAAATCTTGCCTGGAAAGAGGCAGTCTTCTT AGCATGGTGAGCTGAGTTCATGGCTTTTTTTTGTAGCCAGTCCTGTCCCTGGCCATCCAT GTGATGGTTTTGGATGGAGTTAAACTTGATGCCAGTGGGCAGTGCATGTGGAAAGTATCA GAGTAAGGCTCTCCCCTCCAGAGCCCTGAGTTTCTTGGCTGCATGAAGGTTTTCTTTAGA ATCAGAATTGTAGCCAGTTTCTTTGGCCAGAAGGATGAATACTTGGATATTACTGAAAGG GAGGGGTGGAGATGGGTGTGGCAGTGTATGGTGTGTGATTTTTATTTTCTTCTTTGGTCA TGGGGGCCAAGGAGAAAGGCATGAATCTTCCCTGTCAGGCTCTTACAGCCACAGGCACTG TGTCTACTGTCTGGAAGACATGTCCCCGTGGCTGTGGGGCCGCTGCTTCTGTTTAAATAA AAGTGGCCTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_014762 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014762.3, NP_055577.1 |
| RefSeq Size | 4286 bp |
| RefSeq ORF | 1551 bp |
| Locus ID | 1718 |
| UniProt ID | Q15392 |
| Domains | FAD_binding_4 |
| Protein Families | Druggable Genome, Stem cell - Pluripotency, Transmembrane |
| Protein Pathways | Metabolic pathways, Steroid biosynthesis |
| Gene Summary | This gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells. [provided by RefSeq, Jul 2008] |
Citations (2)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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Genkwadaphnin inhibits growth and invasion in hepatocellular carcinoma by blocking DHCR24-mediated cholesterol biosynthesis and lipid rafts formation
,Wu, J;Guo, L;Qiu, X;Ren, Y;Li, F;Cui, W;Song, S;,
Br. J. Cancer
,PubMed ID 32958824
[DHCR24]
|
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Coxiella burnetii expresses a functional {Delta}24 sterol reductase
,Stacey D. Gilk, Paul A. Beare, and Robert A. Heinzen,
J. Bacteriol., Sep 2010; 10.1128/JB.00818-10
[DHCR24]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203507 | DHCR24 (Myc-DDK-tagged)-Human 24-dehydrocholesterol reductase (DHCR24) |
CNY 3,840.00 |
|
| RC203507L1 | Lenti ORF clone of Human 24-dehydrocholesterol reductase (DHCR24), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203507L2 | Lenti ORF clone of Human 24-dehydrocholesterol reductase (DHCR24), mGFP tagged |
CNY 5,890.00 |
|
| RC203507L3 | Lenti ORF clone of Human 24-dehydrocholesterol reductase (DHCR24), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203507L4 | Lenti ORF clone of Human 24-dehydrocholesterol reductase (DHCR24), mGFP tagged |
CNY 5,890.00 |
|
| RG203507 | DHCR24 (tGFP-tagged) - Human 24-dehydrocholesterol reductase (DHCR24) |
CNY 4,370.00 |
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