MNK1 (MKNK1) (NM_003684) Human Untagged Clone
CAT＃: SC323330
MKNK1 (untagged)-Kinase deficient mutant (K78M) of Human MAP kinase interacting serine/threonine kinase 1 (MKNK1), transcript variant 1
CNY 5488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | MNK1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323330 sequence for NM_003684 edited (data generated by NextGen Sequencing)
ATGGTATCTTCTCAAAAGTTGGAAAAACCTATAGAGATGGGCAGTAGCGAACCCCTTCCC ATCGCAGATGGTGACAGGAGGAGGAAGAAGAAGCGGAGGGGCCGGGCCACTGACTCCTTG CCAGGAAAGTTTGAAGATATGTACAAGCTGACCTCTGAATTGCTTGGAGAGGGAGCCTAT GCCAAAGTTCAAGGTGCCGTGAGCCTACAGAATGGCAAAGAGTATGCCGTCATGATCATC GAGAAACAAGCAGGGCACAGTCGGAGTAGGGTGTTTCGAGAGGTGGAGACGCTGTATCAG TGTCAGGGAAACAAGAACATTTTGGAGCTGATTGAGTTCTTTGAAGATGACACAAGGTTT TACTTGGTCTTTGAGAAATTGCAAGGAGGTTCCATCTTAGCCCACATCCAGAAGCAAAAG CACTTCAATGAGCGAGAAGCCAGCCGAGTGGTGCGGGACGTTGCTGCTGCCCTTGACTTC CTGCATACCAAAGACAAAGTCTCTCTCTGTCACCTAGGCTGGAGTGCTATGGCGCCATCA GGGCTCACTGCAGCCCCAACCTCCCTGGGCTCCAGTGATCCTCCCACCTCAGCCTCCCAA GTAGCTGGGACTACAGGCATTGCTCATCGTGATCTGAAACCAGAAAATATATTGTGTGAA TCTCCAGAAAAGGTGTCTCCAGTGAAAATCTGTGACTTTGACTTGGGCAGTGGGATGAAA CTGAACAACTCCTGTACCCCCATAACCACACCAGAGCTGACCACCCCATGTGGCTCTGCA GAATACATGGCCCCTGAGGTAGTGGAGGTCTTCACGGACCAGGCCACATTCTACGACAAG CGCTGTGACCTGTGGAGCCTGGGCGTGGTCCTCTACATCATGCTGAGTGGCTACCCACCC TTCGTGGGTCACTGCGGGGCCGACTGTGGCTGGGACCGGGGCGAGGTCTGCAGGGTGTGC CAGAACAAGCTGTTTGAAAGCATCCAGGAAGGCAAGTATGAGTTTCCTGACAAGGACTGG GCACACATCTCCAGTGAAGCCAAAGACCTCATCTCCAAGCTCCTGGTGCGAGATGCAAAG CAGAGACTTAGCGCCGCCCAAGTTCTGCAGCACCCATGGGTGCAGGGGCAAGCTCCAGAA AAGGGACTCCCCACGCCGCAAGTCCTCCAGAGGAACAGCAGCACAATGGACCTGACGCTC TTCGCAGCTGAGGCCATCGCCCTTAACCGCCAGCTATCTCAGCACGAAGAGAACGAACTA GCAGAGGAGCCAGAGGCACTAGCTGATGGCCTCTGCTCCATGAAGCTTTCCCCTCCCTGC AAGTCACGCCTGGCCCGGAGACGGGCCCTGGCCCAGGCAGGCCGTGGTGAAGACAGGAGC CCGCCCACAGCACTCTGA Clone variation with respect to NM_003684.4 233 a=>t;234 a=>g >OriGene 5' read for mutant NM_003684 unedited
CCCGCCCGTTAGAGCAAAGGGCGGTAGGCGCTGTACGGTGGGAGGCTCTATATAAGCAGAGCTCGTTTAG TGAACCGTCAGAATCTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGACCGCTC CCCGGCGGGAGCCAGCGAAGGTTTCCATGTCAGAGGCCGATGGAGAACTGAAGATTGCCACCTACGCACA AAGGCCATTGAGACACTTCGTGTAGCTGGAAGACACCAACTTCCTGACAGGAGCTTTATTTCATTTGGGA TTTCAAGTTTACAGATGGTATCTTCTCAAAAGTTGGAAAAACCTATAGAGATGGGCAGTAGCGAACCCCT TCCCATCGCAGATGGTGACAGGAGGAGGAAGAAGAAGCGGAGGGGCCGGGCCACTGACTCCTTGCCAGGA AAGTTTGAAGATATGTACAAGCTGACCTCTGAATTGCTTGGAGAGGGAGCCTATGCCAAAGTTCACGTGC CGTGAGCCTACAGATGCAAGAGTATGCCGTCATGATCATCGAGAACAGCAGGCACAGTCGAGTAGGTGTT CGAAGAGGTGAGACCGCTTGTATCATGTCAGAACAGAACATTTGAAGCTGATGAGTTCTTGAAAATGACC CAAGTTTACTGTCTTGGAAATTGCAAGGAGTCATCTAGCCATTCCGAACCAAAGCCATTCATGACCGAAC CACCAATGTGTCGGACTTCCGTCTGCCTGAACTTCTCGATCCAAGACAAGGCTCTCTGTGACCTAGCCGG GATGTCATGCCTAAGGCCTACTGGACCACACCCCTGTCATAGTATCCAACTCAGCCTCAGATGATCGGGA TCAGAATTGCCATGTTATCGAACAGATATTGTGTACTCCGAAAGGTCCCTGGAACTGCGTCACTAGCGCG AGTGACGAGCAATCTG >SC323330 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CYATGMGCAATGGGCGGTAGGCKGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGCGACCGCTCCCCGGCGGGA GCCAGCGAAGGTTTCCATGTCAGAGGCCGATGGAGAACTGAAGATTGCCACCTACGCACAAAGGCCATTG AGACACTTCGTGTAGCTGGAAGACACCAACTTCCTGACAGGAGCT |
| Restriction Sites | Please inquire |
| ACCN | NM_003684 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003684.3, NP_003675.2 |
| RefSeq Size | 2736 bp |
| RefSeq ORF | 1398 bp |
| Locus ID | 8569 |
| UniProt ID | Q9BUB5 |
| Domains | pkinase, TyrKc, S_TKc |
| Protein Families | Druggable Genome, Protein Kinase |
| Protein Pathways | Insulin signaling pathway, MAPK signaling pathway |
| Gene Summary | This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (1) encodes the longest isoform (1). CCDS Note: The coding region has been updated to scale back the N-terminus to one that is more supported by the available transcript data and by conservation across mammals. |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201149 | MKNK1 (Myc-DDK-tagged)-Human MAP kinase interacting serine/threonine kinase 1 (MKNK1), transcript variant 1 |
CNY 5488.00 |
|
| RC201149L1 | Lenti ORF clone of Human MAP kinase interacting serine/threonine kinase 1 (MKNK1), transcript variant 1, Myc-DDK-tagged |
CNY 7888.00 |
|
| RC201149L2 | Lenti ORF clone of Human MAP kinase interacting serine/threonine kinase 1 (MKNK1), transcript variant 1, mGFP tagged |
CNY 7888.00 |
|
| RC201149L3 | Lenti ORF clone of Human MAP kinase interacting serine/threonine kinase 1 (MKNK1), transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC201149L4 | Lenti ORF clone of Human MAP kinase interacting serine/threonine kinase 1 (MKNK1), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RG201149 | MKNK1 (tGFP-tagged) - Human MAP kinase interacting serine/threonine kinase 1 (MKNK1), transcript variant 1 |
CNY 7088.00 |
|
| SC319083 | MKNK1 (untagged)-Human MAP kinase interacting serine/threonine kinase 1 (MKNK1), transcript variant 1 |
CNY 5488.00 |
|
| SC323392 | MKNK1 (untagged)-Kinase deficient mutant (K78M) of Human MAP kinase interacting serine/threonine kinase 1 (MKNK1), transcript variant 1 |
CNY 5488.00 |
