STK22C (TSSK3) (NM_052841) Human Untagged Clone
CAT＃: SC323521
TSSK3 (untagged)-Kinase deficient mutant (K39M) of Human testis-specific serine kinase 3 (TSSK3)
CNY 2400.00
CNY 6270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | SPOGA3; STK22C; STK22D; TSK3 |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC323521 sequence for NM_052841 edited (data generated by NextGen Sequencing)
ATGGAGGACTTTCTGCTCTCCAATGGGTACCAGCTGGGCAAGACCATTGGGGAAGGGACC TACTCAAAAGTCAAAGAAGCATTTTCCAAAAAACACCAAAGAAAAGTGGCAATTATGGTT ATAGACAAGATGGGAGGGCCAGAAGAGTTTATCCAGAGATTCCTCCCTCGGGAGCTCCAA ATCGTCCGTACCCTGGACCACAAGAACATCATCCAGGTGTATGAGATGCTGGAGTCTGCC GACGGGAAAATCTGCCTGGTGATGGAGCTCGCTGAGGGAGGGGATGTCTTTGACTGCGTG CTGAATGGGGGGCCACTGCCTGAAAGCCGGGCCAAGGCCCTCTTCCGTCAGATGGTTGAG GCCATCCGCTACTGCCATGGCTGTGGTGTGGCCCACCGGGACCTCAAATGTGAGAACGCC TTGTTGCAGGGCTTCAACCTGAAGCTGACTGACTTTGGCTTTGCCAAGGTGTTGCCCAAG TCACACCGGGAGCTGAGCCAGACCTTCTGCGGCAGTACAGCCTATGCTGCCCCCGAGGTG CTGCAGGGCATTCCCCACGATAGCAAAAAAGGTGATGTCTGGAGCATGGGTGTGGTCCTG TATGTCATGCTCTGTGCCAGCCTACCTTTTGACGACACAGACATCCCCAAGATGCTGTGG CAGCAGCAGAAGGGGGTGTCCTTCCCCACTCATCTGAGCATCTCGGCCGATTGCCAGGAC CTGCTCAAGAGGCTCCTGGAACCCGATATGATCCTCCGGCCTTCAATTGAAGAAGTTAGT TGGCATCCATGGCTAGCAAGCACTTGA Clone variation with respect to NM_052841.3 116 a=>t;117 a=>g >OriGene 5' read for mutant NM_052841 unedited
ACCGCCCCGCTCTCAGCAAAGGGCGGTAGGCGCTGTACGGCTGGGAGGATCATATTATAAGCAGAGCTTC GTTTAGTTGAACCGTCAGAATCTTTGTAATACGACTCACTATAGGGCGGCCGCGAATACCCGGGATCGGG GACTGGAGGCCTCTGGCCATGGCGCCTGGAGACGGGGTCGCGCAGACCCCGGACACCCGAGGGGGGCGCA GCAGGCCAGGGCAACCCGCCGCGAGGCGGCGTCCACGCCGAGGAGCCGCGCATTCCCGGTCCACACAGAC GCGGCGGAGCGCCCTCCCAGGCGGGACTACAACTCAATGCCTGGCACGGGGGGCGGGCTCGGCGGGTGAC TTAATCCCGGGCTGAGTTTTGGTGGTGGGTAGTTAAGGGCAGGTAAGGGGCACTGCCCATTTTGGGCCAA GGGTCACACCGGCATCTAATTCCCATTTGCCCCCGGAATTTAAAAAAATGTTCTGGCCTGGGTCCTTCCC AAACAACCGGCGGTTTGTTTCCAAACGGAAAAAGGTTTTAACCCCTGGGGGGCCGGCTGCGATATGAATT TCTTTGGGGAAAAAAAGGAACCGGGCCAAGGGCCAAAGTGGGAATTTAAACTTCCCCTCTAAAACCCCCT TTGCCCTAAAAGGGGAAATGGAAAGGGGGGCCCAAAAACATGGGGGGATTTTTTTCGCTTCCCCAGGGGG TCCCCCTCGGGGCCGACACTTTGGGGAAAGGGACCTCTCCTAAATTTCAGAAACACTTTTTTCCCAAAAA CCCCAAAAAAATTGCGCCTTTTGTGTGTTTAACACAATGGGGGGGGCCCAGAAAATTATTCCCCAAATTT TTCCCTCTCAGGAGTGCCACAATAGTCTTCTACCTGGCGACACAGACATCCTCCACTATGTATGATAATG ACGTGCAGTACTCCGCAGCAGAGAAACTCTCGCGTGTAAGAGTAGCTCCTCTCGTGAGAGGAGAGTAGTT CTACATTCACTCGCTGACATAGGAGACGTCTCGTCTATACACGCACGTTAGCGCGCCGCATGCTC >SC323521 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CGCGMGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTC AGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCCCGGGATCGGGGACTGGAGGCCTCTGGC CATGGCGCCTGGAGACGGGGTCGCGCAGACCCCGGACACCCGAGGGGGGCGCAGCAGGCCAGGGCAACCC GCCGCGAGGCGGCGTCCACGCCGAGGAGCCGCGCATTCCCGGTCC |
| Restriction Sites | Please inquire |
| ACCN | NM_052841 |
| Insert Size | 1444 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_052841.3, NP_443073.1 |
| RefSeq Size | 1330 bp |
| RefSeq ORF | 807 bp |
| Locus ID | 81629 |
| UniProt ID | Q96PN8 |
| Protein Families | Druggable Genome, Protein Kinase |
| Gene Summary | This gene encodes a kinase expressed exclusively in the testis that is thought to play a role in either germ cell differentiation or mature sperm function. [provided by RefSeq, Jul 2008] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC206673 | TSSK3 (Myc-DDK-tagged)-Human testis-specific serine kinase 3 (TSSK3) |
CNY 2400.00 |
|
| RC206673L1 | Lenti ORF clone of Human testis-specific serine kinase 3 (TSSK3), Myc-DDK-tagged |
CNY 4800.00 |
|
| RC206673L2 | Lenti ORF clone of Human testis-specific serine kinase 3 (TSSK3), mGFP tagged |
CNY 5890.00 |
|
| RC206673L3 | Lenti ORF clone of Human testis-specific serine kinase 3 (TSSK3), Myc-DDK-tagged |
CNY 5890.00 |
|
| RC206673L4 | Lenti ORF clone of Human testis-specific serine kinase 3 (TSSK3), mGFP tagged |
CNY 5890.00 |
|
| RG206673 | TSSK3 (tGFP-tagged) - Human testis-specific serine kinase 3 (TSSK3) |
CNY 4370.00 |
|
| SC120138 | TSSK3 (untagged)-Human testis-specific serine kinase 3 (TSSK3) |
CNY 2400.00 |
