ARFGAP3 (NM_014570) Human Untagged Clone
CAT＃: SC324381
ARFGAP3 (untagged)-Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1
CNY 3848.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ARFGAP1 |
| Vector | pCMV6-AC |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>OriGene sequence for NM_014570.3
GCCAGCTGCGCCGCGGCTCACAGCTGACGATGGGGGACCCCAGCAAGCAGGACATCTTGA
CCATCTTCAAGCGCCTCCGCTCGGTGCCCACTAACAAGGTGTGTTTTGATTGTGGTGCCA AAAATCCCAGCTGGGCAAGCATAACCTATGGAGTGTTCCTTTGCATTGATTGCTCAGGGT CCCACCGGTCACTTGGTGTTCACTTGAGTTTTATTCGATCTACAGAGTTGGATTCCAACT GGTCATGGTTTCAGTTGCGATGCATGCAAGTCGGAGGAAACGCTAGTGCATCTTCCTTTT TTCATCAACATGGGTGTTCCACCAATGACACCAATGCCAAGTACAACAGTCGTGCTGCTC AGCTCTATAGGGAGAAAATCAAATCGCTCGCCTCTCAAGCAACACGGAAGCATGGCACTG ATCTGTGGCTTGATAGTTGTGTGGTTCCACCTTTGTCCCCTCCACCAAAGGAGGAAGATT TTTTTGCCTCTCACGTTTCTCCTGAGGTGAGTGACACAGCGTGGGCATCAGCAATAGCAG AACCATCTTCTTTAACATCAAGGCCTGTGGAAACCACTTTGGAAAATAATGAAGGTGGAC AAGAGCAAGGACCAAGTGTGGAAGGTCTTAATGTACCAACAAAGGCTACTTTAGAGGTAT CCTCTATCATAAAAAAGAAACCAAATCAAGCTAAAAAAGGCCTTGGGGCCAAAAAAGGAA GTTTGGGAGCTCAGAAACTGGCAAACACATGCTTTAATGAAATTGAAAAACAAGCTCAAG CTGCGGATAAAATGAAGGAGCAGGAAGACCTGGCCAAGGTGGTATCTAAAGAAGAATCAA TTGTTTCATCATTACGATTAGCCTATAAGGATCTTGAAATTCAAATGAAGAAAGACGAAA AGATGAACATTAGTGGCAAAAAAAATGTTGACTCAGACAGACTCGGCATGGGATTTGGAA ATTGCAGAAGTGTTATTTCACATTCAGTGACTTCAGATATGCAGACCATAGAGCAGGAAT CACCCATTATGGCAAAACCAAGAAAAAAGTATAATGATGACAGTGACGATTCATATTTTA CTTCCAGCTCAAGGTACTTTGACGAGCCAGTGGAGTTAAGGAGCAGTTCTTTCTCTAGCT GGGATGACAGTTCAGATTCCTATTGGAAAAAAGAGACCAGCAAAGATACTGAAACAGTTC TGAAAACCACAGGCTATTCAGACAGACCTACTGCTCGCCGCAAGCCAGATTATGAGCCAG TTGAAAATACAGATGAGGCCCAGAAGAAGTTTGGCAATGTCAAGGCCATTTCATCAGATA TGTATTTTGGAAGACAATCCCAGGCTGATTATGAGACCAGGGCCCGCCTAGAGAGGCTGT CAGCAAGTTCCTCCATAAGCTCGGCTGATCTGTTCGAGGAGCCGAGGAAGCAGCCAGCAG GGAACTACAGCCTGTCCAGCGTGCTGCCCAACGCCCCCGACATGGCGCAGTTCAAGCAGG GAGTGAGATCGGTTGCTGGAAAACTCTCCGTCTTTGCTAATGGAGTCGTGACTTCAATTC AGGATCGCTACGGTTCTTAATACTGAAGTCATGATGTGTATTTCCTGGAGAAATTCCTCT TTAAATGAACAAGTAACCACATCTCAGGCGGCAGTGAAGTCCAGATAGTTTTGCAGATTG TTTTGCTACTTTTTCATATGGTATATGTTTCTGATTTTTAATATTTCTTTTGAGAAATTC TGAGTTCTGATGTAGGAGCTTTCCTGTGATTTCTGTTTCACGTTCCTTCCTGTCACACCC TCCTTTGGCGTCTCTGTGTATATCCTTGCTTTATTTTCTTGGAACCTTTGGTTTCAACAC TGAGGGCCTGGAGACCTCGGCTCCTCCTGCTCCTGAACCAGGAGGCTTCATGTGGGGGAG GAGGAGAGGTCTCCATGTGACACATGGGCTCAGGGCTGCCAGAATCAGCGGATGCTGGAT GGGCCTGCAGAAACAACACTCACCACACACACTTCCTTCAAAAGACCAAAAGTGACTGGT GTCTCGTGTGACAGATTGCTTCATTTATGTTTCTACATAGTAAGGTGACTGCCAAATAAT ATTTGAAGTCATCTGTCTCTTTGTAAATTATTTTATATGACCTATAAATTTAAAAATGTT TTTCAGTGAGTGCTTTTAACAAACTTAAGCTTCTGCCCTGCCAAGGGAATTAATGTTATC TTGTGAAAGGTGTTGCTGTTTGAATTGATGAGAAATGGAAGATGAGAACTCCCTAAGAGT TCTCATAATAAATCATCTCATCACAAATCAATACGGTATACAGAGTTAAAGTGGAATGAG GTAAGAAGATACAGCTACAGAAAATAGTTGCGTGTATGGGAGAACAGTCATTGTAATTGG GTAGTTTTGTTAATAAATATTTTTAAATCTTGCTTTTCAGAAATTACCGAATGTGTATAA ACAAATAAAGAAAAATAATTTAGCTGTGTTTTAGACAGCATTAGAATATATTGTTCAGCA CAGTAAAATATATTTGAAATTTGATAAGCCAAAAATGTGGTTTTGAATGAATATTTTGTG AATCTTTCTTAAAAGCTCAAATTTGTAGACTTCTAAATAGAATAAACACTTGCAGCAGAA AAAAAAAAAAAAAAAAA |
| Restriction Sites | Please inquire |
| ACCN | NM_014570 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014570.3, NP_055385.2 |
| RefSeq Size | 2725 bp |
| RefSeq ORF | 1551 bp |
| Locus ID | 26286 |
| UniProt ID | Q9NP61 |
| Domains | ArfGap |
| Protein Pathways | Endocytosis |
| Gene Summary | The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. [provided by RefSeq, Nov 2008] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202392 | ARFGAP3 (Myc-DDK-tagged)-Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1 |
CNY 3840.00 |
|
| RC202392L3 | Lenti ORF clone of Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC202392L4 | Lenti ORF clone of Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1, mGFP tagged |
CNY 5890.00 |
|
| RG202392 | ARFGAP3 (tGFP-tagged) - Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1 |
CNY 5440.00 |
|
| SC114939 | ARFGAP3 (untagged)-Human ADP-ribosylation factor GTPase activating protein 3 (ARFGAP3), transcript variant 1 |
CNY 3848.00 |
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