SEC13L1 (SEC13) (NM_001136232) Human Untagged Clone
CAT#: SC324922
SEC13 (untagged)-Human SEC13 homolog (S. cerevisiae) (SEC13), transcript variant 2
CNY 6270.00
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Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | D3S1231E; npp-20; SEC13L1; SEC13R |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>SC324922 representing NM_001136232.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGATTCACGACGCCCAGATGGACTACTATGGCACCCGCCTGGCAACCTGCTCATCAGACAGGTCCGTC AAAATCTTTGATGTGCGCAATGGAGGGCAGATCCTTATCGCCGACCTCAGGGGTCATGAGGGTCCTGTG TGGCAAGTGGCCTGGGCTCACCCCATGTACGGCAACATCCTGGCATCGTGCTCCTATGACCGGAAAGTC ATTATCTGGAGAGAGGAAAACGGCACCTGGGAGAAGAGCCACGAGCATGCGGGACACGACTCCTCAGTG AACTCGGTGTGCTGGGCCCCCCATGACTACGGCCTGATCCTGGCCTGTGGGAGCTCGGATGGGGCCATC TCCCTGCTGACTTACACCGGGGAAGGCCAATGGGAAGTAAAGAAGATCAACAACGCTCACACCATTGGC TGCAATGCCGTCAGCTGGGCCCCTGCTGTTGTACCTGGAAGCCTCATAGACCACCCATCGGGGCAGAAA CCCAATTACATCAAGAGGTTTGCATCAGGTGGCTGTGACAACCTCATCAAGCTGTGGAAGGAGGAGGAG GACGGCCAGTGGAAGGAGGAGCAGAAGCTAGAAGCGCACAGTGACTGGGTTCGAGATGTGGCCTGGGCC CCCTCCATCGGCCTGCCCACCAGCACCATCGCCAGCTGCTCCCAGGATGGTCGTGTGTTCATTTGGACC TGTGATGATGCCTCAAGCAATACGTGGTCCCCTAAATTGTTGCACAAGTTCAACGATGTGGTGTGGCAT GTGAGCTGGTCCATCACAGCCAACATCCTGGCTGTCTCTGGTGGAGACAATAAGGTGACCCTGTGGAAG GAGTCAGTTGATGGGCAGTGGGTGTGCATCAGTGATGTCAACAAGGGCCAGGGCTCCGTATCAGCATCA GTGACAGAGGGCCAGCAGAACGAGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
Restriction Sites | SgfI-MluI |
ACCN | NM_001136232 |
Insert Size | 927 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001136232.2 |
RefSeq Size | 1469 bp |
RefSeq ORF | 927 bp |
Locus ID | 6396 |
UniProt ID | P55735 |
MW | 34 kDa |
Gene Summary | The protein encoded by this gene belongs to the SEC13 family of WD-repeat proteins. It is a constituent of the endoplasmic reticulum and the nuclear pore complex. It has similarity to the yeast SEC13 protein, which is required for vesicle biogenesis from endoplasmic reticulum during the transport of proteins. Multiple alternatively spliced transcript variants have been found. [provided by RefSeq, Oct 2008] Transcript Variant: This variant (2) differs in the 5' UTR and the 5' coding region, and initiates translation at a downstream start codon compared to variant 3. It encodes isoform 2 which has a shorter N-terminus, compared to isoform 3. |
Documents
Product Manuals |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC226933 | SEC13 (Myc-DDK-tagged)-Human SEC13 homolog (S. cerevisiae) (SEC13), transcript variant 2 |
CNY 2400.00 |
|
RC226933L3 | Lenti ORF clone of Human SEC13 homolog (S. cerevisiae) (SEC13), transcript variant 2, Myc-DDK-tagged |
CNY 5890.00 |
|
RC226933L4 | Lenti ORF clone of Human SEC13 homolog (S. cerevisiae) (SEC13), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
RG226933 | SEC13 (tGFP-tagged) - Human SEC13 homolog (S. cerevisiae) (SEC13), transcript variant 2 |
CNY 4000.00 |
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