TCHP (NM_001143852) Human Untagged Clone
CAT＃: SC325135
TCHP (untagged)-Human trichoplein, keratin filament binding (TCHP), transcript variant 2
CNY 6270.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | TpMs |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC325135 representing NM_001143852.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCGCTCCCGACGCTGCCGTCCTACTGGTGCAGCCAGCAGCGCCTGAATCAGCAGCTAGCACGACAG CGAGAGCAGGAGGCCCGGCTTCGGCAGCAGTGGGAGCAGAACAGCCGTTACTTCAGGATGTCTGACATC TGCAGCTCCAAACAGGCAGAATGGAGCTCTAAAACCTCCTACCAGCGGAGCATGCATGCCTATCAGCGG GAGAAGATGAAGGAGGAGAAGAGGAGGAGTCTGGAGGCCCGACGGGAAAAGCTCAGGCAGCTCATGCAG GAGGAGCAGGACCTGCTGGCCAGAGAACTGGAGGAGCTGAGGCTGAGCATGAACTTGCAGGAAAGAAGA ATCCGGGAGCAGCACGGGAAGCTGAAATCAGCCAAAGAAGAGCAGAGGAAACTGATTGCTGAACAACTT TTGTACGAACACTGGAAAAAGAACAACCCGAAACTTCGAGAGATGGAGCTGGACCTTCACCAGAAGCAT GTCGTAAACTCTTGGGAAATGCAGAAAGAAGAAAAAAAACAGCAAGAAGCCACCGCAGAGCAAGAGAAC AAACGGTATGAAAATGAATATGAAAGGGCCCGAAGGGAGGCGCTAGAAAGGATGAAAGCTGAAGAGGAG AGGAGGCAGCTGGAGGACAAGCTCCAGGCCGAGGCACTGCTGCAACAGATGGAGGAGCTGAAGCTGAAG GAGGTGGAGGCGACCAAACTAAAGAAGGAGCAGGAGAATCTGTTGAAGCAGCGGTGGGAGCTAGAGAGG CTGGAGGAAGAGCGAAAGCAGATGGAAGCCTTCCGGCAGAAGGCAGAGCTGGGGCGTTTCTTGAGACAT CAGTATAACGCTCAACTCAGCAGACGCACACAGCAGATCCAAGAGGAGCTGGAGGCAGACAGGCGGATC CTGCAGGCCCTCCTCGAGAAGGAGGACGAGAGCCAGCGCCTCCACCTGGCCAGGCGGGAGCAGGTCATG GCCGATGTGGCCTGGATGAAGCAGGCCATTGAGGAGCAGCTGCAGCTGGAGCGGGCGCGGGAGGCAGAG CTGCAGATGCTGCTGAGGGAGGAGGCCAAGGAGATGTGGGAAAAGAGAGAGGCAGAGTGGGCCCGAGAG CGCAGCGCACGGGACAGACTGATGAGCGAGGTTCTGACAGGGAGACAACAGCAAATACAAGAGAAGATT GAGCAGAACCGACGGGCACAAGAGGAATCCCTGAAACACAGGGAGCAACTTATTCGAAATCTTGAGGAG GTGAGAGAGTTGGCTCGTCGCGAGAAAGAGGAGAGTGAAAAGCTGAAATCGGCCAGGAAGCAGGAGCTG GAAGCCCAGGTTGCAGAGCGCCGGCTGCAGGCATGGGAAGCAGACCAGCAGGAGGAGGAGGAAGAGGAG GAGGCCCGGCGGGTCGAGCAGCTCTCAGATGCCCTGCTGCAGCAGGAGGCGGAGACTATGGCTGAGCAG GGCTACCGGCCTAAGCCTTACGGACATCCAAAAATTGCTTGGAACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001143852 |
| Insert Size | 1497 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001143852.1 |
| RefSeq Size | 3111 bp |
| RefSeq ORF | 1497 bp |
| Locus ID | 84260 |
| UniProt ID | Q9BT92 |
| MW | 61.1 kDa |
| Gene Summary | Tumor suppressor which has the ability to inhibit cell growth and be pro-apoptotic during cell stress. Inhibits cell growth in bladder and prostate cancer cells by a down-regulation of HSPB1 by inhibiting its phosphorylation. May act as a 'capping' or 'branching' protein for keratin filaments in the cell periphery. May regulate K8/K18 filament and desmosome organization mainly at the apical or peripheral regions of simple epithelial cells (PubMed:15731013, PubMed:18931701). Is a negative regulator of ciliogenesis (PubMed:25270598).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
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ERK/Ribosomal S6 Kinase (RSK) Signaling Positively Regulates Death Receptor 5 Expression through Co-activation of CHOP and Elk1
,You-Take Oh, Xiangguo Liu, Ping Yue, Sumin Kang, Jing Chen, Jack Taunton, Fadlo R. Khuri, and Shi-Yong Sun,
J. Biol. Chem., Dec 2010; 285: 41310 - 41319
[TCHP]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227291 | TCHP (Myc-DDK-tagged)-Human trichoplein, keratin filament binding (TCHP), transcript variant 2 |
CNY 3656.00 |
|
| RC227291L3 | Lenti ORF clone of Human trichoplein, keratin filament binding (TCHP), transcript variant 2, Myc-DDK-tagged |
CNY 5890.00 |
|
| RC227291L4 | Lenti ORF clone of Human trichoplein, keratin filament binding (TCHP), transcript variant 2, mGFP tagged |
CNY 5890.00 |
|
| RG227291 | TCHP (tGFP-tagged) - Human trichoplein, keratin filament binding (TCHP), transcript variant 2 |
CNY 4370.00 |
