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PRKRA (NM_001139517) Human Untagged Clone
CAT＃: SC325742
PRKRA (untagged)-Human protein kinase, interferon-inducible double stranded RNA dependent activator (PRKRA), transcript variant 2
CNY 6,270.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | DYT16; HSD14; PACT; RAX |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC325742 representing NM_001139517.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCAAAGCACACCTTTTTGTGGTTTTTGCAGTTTGGGGAAGATGATAACAGCTAAGCCAGGGAAAACA CCGATTCAGGTATTACACGAATACGGCATGAAGACCAAGAACATCCCAGTTTATGAATGTGAAAGATCT GATGTGCAAATACACGTGCCCACTTTCACCTTCAGAGTAACCGTTGGTGACATAACCTGCACAGGTGAA GGTACAAGTAAGAAGCTGGCGAAACATAGAGCTGCAGAGGCTGCCATAAACATTTTGAAAGCCAATGCA AGTATTTGCTTTGCAGTTCCTGACCCCTTAATGCCTGACCCTTCCAAGCAACCAAAGAACCAGCTTAAT CCTATTGGTTCATTACAGGAATTGGCTATTCATCATGGCTGGAGACTTCCTGAATATACCCTTTCCCAG GAGGGAGGACCTGCTCATAAGAGAGAATATACTACAATTTGCAGGCTAGAGTCATTTATGGAAACTGGA AAGGGGGCATCAAAAAAGCAAGCCAAAAGGAATGCTGCTGAGAAATTTCTTGCCAAATTTAGTAATATT TCTCCAGAGAACCACATTTCTTTAACAAATGTAGTAGGACATTCTTTAGGATGTACTTGGCATTCCTTG AGGAATTCTCCTGGTGAAAAGATCAACTTACTGAAAAGAAGCCTCCTTAGTATTCCAAATACAGATTAC ATCCAGCTGCTTAGTGAAATTGCCAAGGAACAAGGTTTTAATATAACATATTTGGATATAGATGAACTG AGCGCCAATGGACAATATCAATGTCTTGCTGAACTGTCCACCAGCCCCATCACAGTCTGTCATGGCTCC GGTATCTCCTGTGGCAATGCACAAAGTGATGCAGCTCACAATGCTTTGCAGTATTTAAAGATAATAGCA GAAAGAAAGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001139517 |
| Insert Size | 909 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001139517.1 |
| RefSeq Size | 1906 bp |
| RefSeq ORF | 909 bp |
| Locus ID | 8575 |
| UniProt ID | O75569 |
| Protein Families | Druggable Genome |
| MW | 33.1 kDa |
| Gene Summary | This gene encodes a protein kinase activated by double-stranded RNA which mediates the effects of interferon in response to viral infection. Mutations in this gene have been associated with dystonia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2008] Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. This difference causes translation initiation at a downstream AUG and an isoform (2) with a shorter N-terminus compared to isoform 1. |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227693 | PRKRA (Myc-DDK-tagged)-Human protein kinase, interferon-inducible double stranded RNA dependent activator (PRKRA), transcript variant 2 |
CNY 3,600.00 |
|
| RC227693L3 | Lenti ORF clone of Human protein kinase, interferon-inducible double stranded RNA dependent activator (PRKRA), transcript variant 2, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC227693L4 | Lenti ORF clone of Human protein kinase, interferon-inducible double stranded RNA dependent activator (PRKRA), transcript variant 2, mGFP tagged |
CNY 5,890.00 |
|
| RG227693 | PRKRA (tGFP-tagged) - Human protein kinase, interferon-inducible double stranded RNA dependent activator (PRKRA), transcript variant 2 |
CNY 4,370.00 |
