TLK1 (NM_001136555) Human Untagged Clone
CAT＃: SC326160
TLK1 (untagged)-Human tousled-like kinase 1 (TLK1), transcript variant 3
CNY 13020.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | PKU-beta |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC326160 representing NM_001136555.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCTGTTTTATTCTTGTATGATTTGAAAACTACTGGCAAGACACCGGAGAAGAAACAATCGGAATCA TCCAGGGGAAGAAAGAGAAAAGCAGAAAACCAGAATGAAAGTAGTCAGGGAAAAAGTATTGGGGGACGT GGCCACAAAATTAGCGACTATTTTGAATACCAGGGTGGAAATGGCTCAAGTCCAGTAAGAGGCATACCT CCTGCAATCCGTTCTCCTCAAAATTCACATTCACATTCCACTCCTTCCTCATCTGTTCGACCGAATAGC CCTTCTCCTACTGCATTAGCATTTGGGGACCACCCTATTGTACAACCAAAGCAATTATCCTTTAAAATT ATTCAGACTGATCTCACAATGCTGAAATTAGCAGCATTAGAAAGTAATAAAATCCAGGACCTGGAAAAG AAGGAGGGACGTATAGATGATTTGCTCAGGGCTAACTGTGATCTCAGACGGCAAATAGATGAACAACAA AAATTACTTGAAAAATACAAAGAACGATTAAATAAGTGCATATCAATGAGCAAGAAACTTCTTATTGAA AAGAGTACACAAGAAAAGCTGTCAAGCAGAGAGAAGAGTATGCAAGATCGATTACGCCTCGGGCACTTT ACAACAGTTAGACATGGCGCTTCATTTACTGAACAATGGACAGATGGTTTTGCATTTCAGAATCTTGTG AAGCAACAAGAATGGGTGAATCAGCAAAGGGAAGATATTGAAAGGCAAAGGAAACTTCTAGCCAAACGC AAACCTCCCACAGCTAATAATTCTCAGGCACCCTCTACCAATTCTGAACCAAAACAAAGGAAAAACAAA GCAGTCAATGGAGCAGAGAATGATCCCTTTGTTAGACCAAATTTACCACAACTGTTGACTTTGGCAGAA TATCATGAACAGGAAGAAATTTTCAAACTTAGACTAGGACATCTCAAAAAGGAAGAGGCAGAAATCCAG GCAGAACTTGAACGTTTGGAAAGAGTCAGAAATCTTCACATACGTGAGCTGAAAAGAATAAACAATGAA GATAATTCACAGTTCAAAGATCACCCAACATTAAATGAAAGATATTTATTACTTCATCTGCTTGGTAGA GGTGGCTTTAGTGAAGTGTATAAGGCTTTTGACCTTTATGAACAAAGATATGCTGCTGTGAAGATACAT CAGCTTAATAAAAGCTGGAGAGATGAGAAGAAAGAAAACTACCACAAACATGCCTGCAGAGAGTATAGA ATACACAAAGAACTGGATCACCCCAGAATAGTTAAACTCTATGATTATTTCTCCTTGGATACAGATACG TTTTGTACAGTGTTAGAATACTGTGAAGGCAATGACTTGGATTTCTATCTGAAGCAACACAAGTTAATG TCAGAGAAAGAAGCTCGGTCTATTGTAATGCAGATTGTAAATGCACTAAGATATCTCAATGAGATCAAA CCCCCTATTATACATTATGATCTTAAGCCAGGAAACATCCTACTGGTAGATGGAACAGCATGTGGTGAA ATCAAAATCACTGATTTTGGTCTGTCCAAGATTATGGATGATGATAGCTATGGTGTAGATGGAATGGAT CTAACTTCCCAGGGGGCAGGCACTTACTGGTATTTACCTCCTGAGTGTTTTGTAGTTGGAAAAGAGCCA CCAAAGATTTCCAACAAGGTTGATGTATGGTCGGTTGGAGTCATCTTCTTTCAGTGTCTTTATGGTAGA AAGCCATTTGGTCACAATCAATCTCAACAAGACATTCTTCAAGAAAATACAATATTAAAAGCCACAGAA GTCCAGTTCCCTGTAAAACCGGTTGTAAGCAGTGAAGCCAAGGCATTTATAAGACGCTGTTTGGCATAT CGAAAAGAAGATCGATTTGATGTGCACCAGCTGGCAAATGACCCATACCTTCTCCCACACATGAGAAGA TCAAATTCTTCAGGAAACCTACACATGGCTGGGCTGACAGCATCCCCTACACCCCCTTCTTCAAGCATA ATTACTTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001136555 |
| Insert Size | 2013 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001136555.1 |
| RefSeq Size | 5081 bp |
| RefSeq ORF | 2013 bp |
| Locus ID | 9874 |
| UniProt ID | Q9UKI8 |
| Protein Families | Druggable Genome, Protein Kinase |
| MW | 77.1 kDa |
| Gene Summary | The protein encoded by this gene is a serine/threonine kinase that may be involved in the regulation of chromatin assembly. The encoded protein is only active when it is phosphorylated, and this phosphorylation is cell cycle-dependent, with the maximal activity of this protein coming during S phase. The catalytic activity of this protein is diminished by DNA damage and by blockage of DNA replication. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (3) differs in the 5' UTR and coding region, and uses a downstream start codon, compared to variant 1, resulting in a shorter protein (isoform 3). |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226651 | TLK1 (Myc-DDK-tagged)-Human tousled-like kinase 1 (TLK1), transcript variant 3 |
CNY 4912.00 |
|
| RC226651L3 | Lenti-ORF clone of TLK1 (Myc-DDK-tagged)-Human tousled-like kinase 1 (TLK1), transcript variant 3 |
CNY 9030.00 |
|
| RC226651L4 | Lenti-ORF clone of TLK1 (mGFP-tagged)-Human tousled-like kinase 1 (TLK1), transcript variant 3 |
CNY 9030.00 |
|
| RG226651 | TLK1 (tGFP-tagged) - Human tousled-like kinase 1 (TLK1), transcript variant 3 |
CNY 7890.00 |
