NEDD4 2 (NEDD4L) (NM_001144967) Human Untagged Clone
CAT＃: SC326303
NEDD4L (untagged)-Human neural precursor cell expressed, developmentally down-regulated 4-like (NEDD4L), transcript variant j
CNY 11432.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | hNEDD4-2; NEDD4-2; NEDD4.2; PVNH7; RSP5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001144967 |
| Insert Size | 2928 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001144967.2 |
| RefSeq Size | 8482 bp |
| RefSeq ORF | 2928 bp |
| Locus ID | 23327 |
| UniProt ID | Q96PU5 |
| Protein Families | Druggable Genome |
| Protein Pathways | Endocytosis, Ubiquitin mediated proteolysis |
| MW | 111.9 kDa |
| Gene Summary | This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012] Transcript Variant: This variant (j) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
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Mutations in the HECT domain of NEDD4L lead to AKT-mTOR pathway deregulation and cause periventricular nodular heterotopia
,Broix, L;Jagline, H;L Ivanova, E;Schmucker, S;Drouot, N;Clayton-Smith, J;Pagnamenta, AT;Metcalfe, KA;Isidor, B;Louvier, UW;Poduri, A;Taylor, JC;Tilly, P;Poirier, K;Saillour, Y;Lebrun, N;Stemmelen, T;Rudolf, G;Muraca, G;Saintpierre, B;Elmorjani, A;, ;Moïse, M;Weirauch, NB;Guerrini, R;Boland, A;Olaso, R;Masson, C;Tripathy, R;Keays, D;Beldjord, C;Nguyen, L;Godin, J;Kini, U;Nischké, P;Deleuze, JF;Bahi-Buisson, N;Sumara, I;Hinckelmann, MV;Chelly, J;,
Nat. Genet.
,PubMed ID 27694961
[NEDD4L]
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226610 | NEDD4L (Myc-DDK-tagged)-Human neural precursor cell expressed, developmentally down-regulated 4-like (NEDD4L), transcript variant j |
CNY 9690.00 |
|
| RC226610L3 | Lenti-ORF clone of NEDD4L (Myc-DDK-tagged)-Human neural precursor cell expressed, developmentally down-regulated 4-like (NEDD4L), transcript variant j |
CNY 11590.00 |
|
| RC226610L4 | Lenti-ORF clone of NEDD4L (mGFP-tagged)-Human neural precursor cell expressed, developmentally down-regulated 4-like (NEDD4L), transcript variant j |
CNY 11590.00 |
|
| RG226610 | NEDD4L (tGFP-tagged) - Human neural precursor cell expressed, developmentally down-regulated 4-like (NEDD4L), transcript variant j |
CNY 13024.00 |
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