VWDE (NM_001135924) Human Untagged Clone
CAT＃: SC326391
VWDE (untagged)-Human von Willebrand factor D and EGF domains (VWDE)
CNY 30590.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC326391 representing NM_001135924.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGCCTGGCGGAGCCTGCGTGCTGGTGATCGCGCTGATGTTCCTGGCCTGGGGGGAAGCTCAGGAGTGC TCTCCTGGGGGACACCAGTTTCTTCGGAGTCCTTATAGAAGTGTCCGTTTTGACTCATGGCACCTCCAG CAGTCAGCTGTTCAAGACCTAATATGTGACCATTCCCTCTCCCCTGGATGGTATAGATTTCTCATCCTT GACAGACCTGCCGAGATGCCAACCAAATGTGTTGAGATGAACCATTGTGGAACTCAGGCCCCCATCTGG CTGTCTCTGAGAGATTCAGAAACACTGCCATCTCCTGGGGAGATCAAGCAATTGACAGCTTGTGCAACA TGGCAGTTTTTGTTCAGCACTACAAAAGACTGCTGTCTCTTTCAAATCCCAGTGTCTGTAAGAAACTGT GGGAACTTTTCTGTATACTTACTACAACCAACTCAGGGATGTATGGGCTACTGTGCGGAAGCTATTTCT GATGCACGATTACACCCATGTGGTTCTGATGAAACTGAAACAGGAGGTGATTGTGTTCGTCAGCTGGCT GCCTCATTGCCACCTCCACCTGCAGGAAGGCCAGAGGTTCTGGTGGAGTTGATTGAGTCCAGGCTTTTC TGTAGGTGTTCTTTTGATGTTCCCGCTACAAAAAACTCAGTGGGATTTCACATAGCTTGGTCTAGGCTT TCTTCTCAAGAAGTCAAAGAGGAGCTGACACAAGAGACCACAGTTCAGGCATTCTCTCTTTTAGAACTT GATGGCATAAATCTCAGACTTGGAGACAGGATATTCTGCAGCGCTTCTGTCTTTTTCTTGGAGAATCCT CATGTACAAAGTGTAGCCATCGAAAGCCAAGAATTTTTTGCAGGCTTTAAGCTACAGCCTGAATTGAGC ACTATATCAGAGGATGGGAAAGAATACTACCTGAGGATAGAAAGCACAGTTCCTATTATTTGTTCTGAA TTTAGTGAGCTTGATCAAGAATGCAAAATCTCATTAAAACTGAAAACTATTGGTCAAGGTAGAGAGCAC CTAGGCTTAAATTTGGCACTGTCTTCCTGTCATGTGGACCTTCTCCAGACATCTTCCTGTGCTAATGGA ACCTGTAGCCACACTTTTGTGTACTACACTGCTGTCACAGATTTTTCTCGAGATGGAGATAGAGTCTCA AACATTGTAGTGCAACCAATAGTTAATGAGGATTTCCTGTGGAACAACTACATTCCAGACAGCATCCAG ATCAAAGTAAAGGATGTCCCAACTGCTTACTGCTATACATTTACTGACCCACATATAATTACATTTGAT GGCAGGGTATATGATAATTTCAAGACTGGAACATTTGTGCTTTATAAGAGTATGTCACGTGATTTTGAA GTCCATGTACGTCAGTGGGACTGCAGAAGCCTTCACTATCCAGTGTCATGTAATTGTGGGTTTGTTGCC CAGGAAGGAGGTGATATAGTTACTTTTGATATGTGCAATGGTCAGCTACGTGAATCACAACCATATTTG TTCATAAAAAGCCAAGATGTAACCAGGAATATCAAGATAAGTGAATCTTACTTAGGAAGAAAAGTCACA ATCTGGTTTTCTTCTGGGGCATTTATCCGTGCTGATCTTGGTGAATGGGGCATGAGTCTAACGATCAGA GCCCCTAGTGTAGATTACAGAAACACTCTGGGACTTTGTGGAACCTTTGATGAAAATCCGGAAAATGAT TTCCATGACAAAAATGGGATGCAAATTGATCAAAATTTTAACAATTATGTTGCTTTTATTAATGAATGG AGGATTTTACCAGGAAAAAGCATGTCTGACACACTGCCAGTTTCTATGACATCACCTGGAAAGCCATCC TATTGTAGCTGTTCATTGGACACTGCAGCGTATCCGTCTTCCGAAGATCTGGACAGTGTTTCTCGATCA GAAATTGCCTTGGGTTGCAAAGACCTCAATCATGTCAGCTTATCTTCTTTGATACCAGAACTAGATGTC ACCTCCGAATATATTAATTCAGACACTCTTGTCAGAGAGATAAACAAGCATACATCTCCAGAAGAATAT AATCTAAATTTATTTCTGCAAGAAAAAAAACACATAAACCTGACTAAACTCGGCTTAAATGTACAAAAA CATCCTGGAAATGAGAAAGAAGATTCACTACAATATTTGGCCAATAAGAAATATACACAAGGCCGGGGA AGCCACAGCCAAGAAATGAGGTACAATCGACAAAACAGATGGAAACGGCAGAACTTTCATGAGTTTCCT CCTTTGTTTGCTTTCCCGAGTCTCAGCCAAACGGATCTGGAAGAACTTACTTATTTTTTCCCAGAGGAC CATGCTGAGGATGTACAGCAAGAGTTTTTCCCCTCTTGGCCCACTCCCTCTGGCCTCACCGAGTATAGC ACCTTGACCCTCTGTCAGGAGACTCTAGCCAACTCCAGCATAGGAAGGCTGTGTCTTGCTTTTCTTGGC AAGAGATTAGACAGTGTTATAGAGATGTGTGTGAAGGATGTTCTGTTAAAAGATGATCTTAGTTGGGCA GAAGCAGGTGTGGCCCTTTTAGAAAATGAATGTGAAAAGAGGATTGTGGAGGAGGGGAAATATAACACA GAAGAGTATGGCACATCAATTGAAGACATTCTCTCAGTATTAAAATGCCCCAATTTATGCAGCGGCAAT GGGCAGTGCATGGAATGGGGGTGTGCGTGTTCCCCAAGCTTTAGTTCCTATGACTGCAGTGATTCCTAT GACAAAGCTCCTGAAATTACAGAGCTTGGGAATGCTGGATTCTGTGATGTTCAAAAATATAATTGTATG ATGGTGAGAGTTTTTGGCAAAGGCTTCAAAGAATTACCTTCAATTAAATGTGAAGTTACTAAGCTACAG TATAATAGCAGTGAATGGATGCCTGGAGAACCCATCTATACACAGACTGTTTTCCACAATAGCAGAGCT GTTGATTGTCAGCTGCCCACTGATGTTCAGCAGTTTGATACCATGGATCTGGTGGGTGGGAAACCAACT GGGAAGTGGCAGTTGAAGGTATCTAATGATGGTTATAAATTCAGTAATCCCAAAATAACGGTCATATAT GATGGTGCTTGCCAAGTTTGTGGTCTCTATAAAAATGACTCATGTACTATAAAGGAAAATGTTTGCATT ATTGATGGACTCTGCTATGTTGAAGGAGACAAAAATCCAACCAGCCCTTGTTTGATTTGTAGACCCAAA ATTTCAAGATTTACTTGGTCATTTTTAGAAAACAACCAGCCCCCAGTGATTCAAGCATTGCAAGACAAA TTACAGACATTTTATGGTGAAAACTTTGAGTATCAGTTCGTGGCCTTCGATCCAGAAGGTTCTGACATC CATTTTACGTTGGACTCTGGTCCTGAAGGGGCAAGTGTTTCCTCTGCAGGGCTTTTTATGTGGAAAACA GATTTACTAACTACCCAACAAATTACTGTACGCCTTAATGATGACTGCGATGCTGAAACTAGAGTCACG ATTGAGGTGACTGTGAAGTCTTGTGATTGCTTGAATGGTGGATCATGTGTATCTGATAGGAACTTTTCT CCAGGGAGTGGAGTGTACCTGTGTGTCTGCTTGCCTGGCTTCCATGGCAGCCTTTGTGAAGTGGACATT AGTGGGTGCCAATCCAACCCTTGTGGTCTTGGCAGCTATATTAGTGGTTTTCACAGTTATTCCTGTGAT TGTCCACCTGAGCTCAAAGTTGAAACACAGTTTGTAAATCAATTTACTACACAAACTGTGGTTCTCACA AGATCTGATAAAAGTGTAAATAAAGAAGAGGATGATAAAAATGCCCAAGGGAGAAAGAGGCATGTTAAG CCAACAAGTGGAAATGCCTTCACCATTTGTAAATATCCATGTGGAAAAAGTAGGGAGTGTGTTGCCCCA AACATCTGCAAATGTAAACCTGGTTACATTGGTTCTAACTGCCAAACTGCTCTTTGTGACCCTGATTGC AAAAACCATGGAAAATGTATTAAGCCTAACATTTGTCAGTGTCTTCCAGGACATGGTGGAGCAACCTGT GATGAAGAACATTGTAACCCACCTTGTCAACATGGTGGCACATGCTTGGCTGGGAATCTCTGCACTTGT CCTTATGGTTTTGTAGGACCAAGGTGTGAAACAATGGTTTGTAACAGGCACTGTGAAAATGGAGGCCAG TGTCTTACACCAGATATTTGCCAGTGCAAACCTGGCTGGTATGGACCCACCTGTAGTACAGCTTTGTGC GACCCTGTCTGCCTCAATGGTGGTTCGTGTAATAAGCCAAATACTTGCCTCTGTCCAAATGGATTCTTT GGGGAACACTGTCAGAATGCTTTCTGTCACCCTCCCTGTAAGAATGGTGGCCACTGCATGAGAAATAAT GTGTGCGTCTGTCGTGAAGGATACACTGGTAGGAGATTCCAAAAAAGCATCTGTGATCCTACGTGCATG AATGGAGGAAAATGTGTGGGACCAAGCACTTGCTCTTGTCCTTCTGGTTGGAGTGGGAAACGATGCAAC ACACCTATCTGCTTGCAGAAATGTAAAAACGGTGGTGAATGCATTGCGCCCAGCATATGCCATTGTCCT TCCTCCTGGGAAGGAGTGCGGTGTCAAATACCAATTTGCAACCCAAAATGTCTTTATGGAGGCAGATGC ATATTTCCCAATGTGTGTTCCTGCCGCACTGAATACTCTGGAGTCAAATGTGAGAAGAAAATACAGATA AGACGCCATTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001135924 |
| Insert Size | 4773 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001135924.1 |
| RefSeq Size | 5583 bp |
| RefSeq ORF | 4773 bp |
| Locus ID | 221806 |
| UniProt ID | Q8N2E2 |
| MW | 176.8 kDa |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC227239 | VWDE (Myc-DDK-tagged)-Human von Willebrand factor D and EGF domains (VWDE) |
CNY 10880.00 |
|
| RC227239L3 | Lenti ORF clone of Human von Willebrand factor D and EGF domains (VWDE), Myc-DDK-tagged |
CNY 15680.00 |
|
| RC227239L4 | Lenti ORF clone of Human von Willebrand factor D and EGF domains (VWDE), mGFP tagged |
CNY 15680.00 |
|
| RG227239 | VWDE (tGFP-tagged) - Human von Willebrand factor D and EGF domains (VWDE) |
CNY 15200.00 |
