TNRC6B (NM_001162501) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC327332 representing NM_001162501. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAGAGAGAAGGAGCAAGAAAGGGAAGAACAGTTAATGGAAGACAAGAAAAGGAAGAAAGAGGATAAA AAGAAAAAAGAAGCCACTCAGAAGGTCACGGAACAAAAAACCAAAGTGCCCGAAGTGACGAAACCAAGT TTAAGCCAACCAACGGCCGCCAGCCCAATTGGCAGCTCTCCATCGCCACCAGTCAATGGTGGCAACAAT GCCAAAAGGGTGGCAGTGCCGAACGGACAACCGCCAAGCGCCGCCCGCTACATGCCTCGGGAGGTGCCG CCGCGATTCCGTTGCCAGCAGGACCACAAAGTGTTACTAAAACGTGGGCAGCCCCCTCCACCGTCCTGC ATGCTCCTTGGGGGTGGGGCAGGGCCTCCTCCCTGCACAGCACCTGGAGCAAACCCAAACAACGCACAA GTGACAGGAGCGCTGCTGCAGAGTGAGAGTGGGACTGCGCCAGACTCAACCCTTGGAGGTGCTGCTGCT TCAAATTATGCAAATTCCACTTGGGGCTCGGGAGCCTCCTCCAACAACGGCACCTCCCCCAACCCAATT CACATCTGGGACAAGGTGATTGTAGACGGGTCTGACATGGAAGAGTGGCCTTGTATTGCCAGCAAAGAC ACTGAATCTTCTTCCGAAAACACCACCGATAACAACAGTGCCTCGAACCCTGGCTCTGAGAAGAGCACT CTGCCAGGAAGCACCACTAGTAACAAAGGAAAAGGGAGCCAGTGCCAGTCTGCAAGTTCTGGGAACGAA TGTAATCTTGGGGTCTGGAAATCTGACCCTAAGGCTAAATCTGTTCAATCTTCCAACTCTACTACAGAG AACAACAATGGACTAGGAAATTGGAGGAATGTGAGTGGTCAGGATAGAATTGGACCTGGCTCTGGCTTC AGCAACTTTAACCCAAATAGCAACCCATCTGCCTGGCCAGCACTGGTCCAAGAAGGAACTTCTAGGAAA GGGGCATTGGAAACAGATAATAGTAATTCCAGTGCACAGGTTAGCACAGTAGGTCAGACATCCAGGGAA CAGCAGTCAAAGATGGAAAATGCGGGTGTTAATTTTGTTGTCTCTGGCAGAGAACAGGCTCAAATTCAT AACACTGATGGACCAAAAAATGGAAACACTAACTCCTTGAACTTAAGTTCACCAAACCCCATGGAGAAT AAGGGAATGCCCTTTGGAATGGGCTTGGGGAACACCTCCAGGAGCACTGATGCCCCTTCACAAAGCACT GGAGATCGAAAGACTGGGAGTGTTGGATCTTGGGGTGCAGCTAGGGGGCCTTCTGGAACTGACACAGTC TCTGGACAAAGCAATTCTGGAAACAATGGGAACAATGGAAAAGAGAGAGAGGACTCCTGGAAAGGAGCT TCTGTTCAGAAATCAACTGGGTCAAAAAATGACTCTTGGGACAACAATAACAGGTCTACGGGTGGGTCC TGGAACTTTGGCCCCCAGGACTCTAATGACAACAAATGGGGTGAAGGGAACAAAATGACATCTGGGGTC TCTCAGGGAGAATGGAAACAGCCGACTGGGTCTGATGAGTTGAAAATTGGAGAATGGAGTGGTCCAAAC CAACCAAATTCTAGCACTGGAGCATGGGACAATCAAAAGGGCCACCCCCTCCCTGAAAACCAAGGCAAT GCCCAGGCTCCCTGTTGGGGAAGATCTTCCAGCTCCACAGGAAGTGAAGTTGGAGGTCAAAGCACTGGA AGCAACCACAAAGCAGGAAGTAGTGACAGTCATAACTCTGGCCGTCGGTCGTACAGGCCCACACATCCT GATTGTCAGGCTGTCTTGCAGACTCTTTTGAGCCGAACTGATTTGGACCCCAGGGTGCTCTCAAACACT GGCTGGGGCCAAACTCAAATTAAGCAGGACACAGTGTGGGACATTGAAGAGGTGCCAAGGCCTGAGGGG AAATCTGACAAAGGAACTGAGGGGTGGGAGAGCGCTGCCACACAGACCAAGAACTCAGGGGGCTGGGGA GATGCACCCAGCCAAAGCAATCAAATGAAGTCTGGATGGGGGGAGCTCTCAGCCTCTACAGAGTGGAAA GACCCCAAGAACACAGGAGGCTGGAATGACTACAAGAACAACAACTCTTCCAACTGGGGAGGAGGACGA CCTGATGAAAAGACACCTTCCTCTTGGAATGAGAATCCCAGCAAGGATCAGGGGTGGGGAGGTGGACGC CAGCCCAATCAAGGATGGTCTTCTGGAAAGAATGGTTGGGGGGAGGAAGTCGATCAGACAAAAAACAGC AATTGGGAAAGTTCTGCAAGTAAACCTGTGTCTGGGTGGGGTGAAGGAGGGCAGAATGAAATCGGGACT TGGGGTAATGGTGGCAATGCAAGCCTAGCTTCAAAAGGTGGGTGGGAGGATTGCAAAAGATCCCCAGCA TGGAATGAGACGGGCCGACAGCCCAATTCCTGGAATAAACAACACCAACAGCAGCAGCCCCCACAGCAG CCGCCGCCACCACAACCAGAGGCTTCTGGTTCGTGGGGAGGCCCACCCCCACCACCTCCAGGCAACGTT CGACCTTCCAATTCCAGCTGGAGCAGCGGGCCACAGCCTGCAACACCTAAGGATGAGGAACCCAGTGGT TGGGAAGAGCCATCCCCACAGTCAATTAGTCGGAAAATGGACATTGATGATGGCACTTCAGCATGGGGA GACCCTAACAGTTATAACTACAAGAATGTGAATCTGTGGGATAAGAATTCCCAAGGGGGCCCAGCACCT CGAGAACCAAACCTGCCCACCCCAATGACCAGTAAATCGGCATCAGTCTGGAGCAAAAGCACACCACCT GCTCCAGATAATGGTACTTCCGCTTGGGGTGAGCCAAATGAAAGCAGTCCTGGGTGGGGCGAGATGGAT GATACAGGAGCATCGACCACAGGCTGGGGGAACACGCCCGCCAACGCTCCCAATGCCATGAAGCCTAAT TCCAAATCTATGCAAGACGGCTGGGGGGAGAGTGACGGGCCAGTCACAGGAGCTCGCCATCCCAGCTGG GAAGAGGAGGAGGATGGAGGAGTCTGGAACACCACTGGCTCTCAGGGCAGTGCTTCCTCCCACAACTCA GCAAGCTGGGGACAAGGAGGAAAGAAACAAATGAAGTGCTCACTCAAAGGAGGAAACAATGATTCATGG ATGAATCCTCTTGCCAAACAGTTTTCAAATATGGGATTGCTGAGTCAGACTGAAGATAATCCAAGCAGC AAAATGGATTTGTCTGTAGGAAGCCTTTCAGATAAAAAATTTGATGTGGACAAGCGAGCGATGAATCTC GGGGATTTTAATGATATCATGAGGAAGGATCGATCTGGGTTCCGTCCACCTAATTCCAAAGACATGGGA ACCACAGATAGTGGGCCTTATTTTGAGAAGCTGACTTTGCCTTTCTCCAATCAAGATGGGTGCCTTGGG GATGAGGCTCCCTGCTCTCCCTTCTCCCCTTCTCCCAGCTACAAGCTGTCTCCCTCTGGTTCCACACTA CCCAACGTCAGCCTTGGAGCAATCGGCACAGGGCTCAACCCCCAAAACTTCGCTGCTAGACAAGGCGGT AGTCATGGTTTGTTTGGAAACAGCACAGCACAATCGAGAGGTCTGCACACACCCGTGCAGCCACTAAAT TCTTCTCCCAGTCTCCGGGCGCAAGTGCCTCCCCAGTTTATTTCCCCCCAGGTTTCTGCCTCAATGCTC AAGCAGTTTCCCAACAGTGGCCTGAGTCCAGGTCTTTTCAATGTGGGGCCCCAGTTATCTCCTCAACAA ATTGCCATGCTGAGCCAGCTTCCACAAATTCCCCAGTTTCAGTTGGCATGTCAGCTTCTCTTGCAGCAG CAGCAACAGCAGCAGTTGTTACAGAACCAGAGAAAGATTTCTCAAGCTGTACGCCAACAGCAAGAGCAG CAGCTGGCTCGAATGGTGAGTGCACTGCAGCAGCAGCAGCAGCAGCAGCAGAGGCAGCCAGGCATGAAG CACTCGCCCTCTCATCCTGTTGGGCCCAAGCCGCATCTGGACAACATGGTACCCAACGCATTGAATGTG GGGCTCCCAGACCTTCAAACCAAAGGGCCAATACCTGGATATGGTTCTGGCTTCAGCTCTGGCGGCATG GACTATGGCATGGTTGGTGGGAAGGAGGCTGGAACCGAGTCTCGCTTTAAACAGTGGACCTCCATGATG GAGGGGCTGCCCTCTGTAGCCACACAGGAAGCCAATATGCACAAAAATGGCGCTATAGTGGCCCCTGGT AAAACCCGGGGAGGGTCACCGTACAACCAGTTTGATATCATCCCTGGTGACACACTGGGTGGCCATACG GGTCCTGCTGGTGATAGCTGGTTACCTGCCAAATCTCCACCAACAAATAAAATCGGAAGTAAATCCAGC AATGCCAGTTGGCCTCCAGAATTCCAACCAGGAGTGCCATGGAAAGGTATCCAAAACATTGACCCTGAA TCTGACCCCTATGTCACCCCAGGAAGTGTGCTGGGGGGTACAGCCACATCTCCCATTGTAGATACTGAC CACCAACTGCTGCGGGATAACACCACAGGGTCTAATTCTTCCCTCAACACCTCGCTGCCTTCACCTGGT GCCTGGCCCTACAGTGCCTCTGACAACTCCTTTACCAACGTTCATAGCACTTCAGCAAAGTTCCCTGAT TACAAATCAACATGGTCCCCAGATCCCATAGGACACAACCCCACTCATCTCTCCAACAAGATGTGGAAA AACCATATTTCCTCCAGGAACACTACACCGCTGCCCCGCCCACCTCCTGGTCTGACCAACCCCAAACCA TCATCTCCCTGGAGCAGCACAGCACCCCGATCAGTCAGGGGGTGGGGGACACAGGACTCACGGCTCGCC TCGGCCTCTACCTGGAGTGATGGTGGCTCAGTTCGTCCTAGTTACTGGCTGGTTCTTCACAATCTCACC CCACAGATTGATGGGTCAACCTTGAGAACGATCTGCATGCAGCATGGCCCACTGCTGACATTCCATCTG AATCTAACCCAGGGCACTGCCCTGATCCGATACAGCACCAAACAGGAGGCGGCCAAGGCCCAAACTGCA CTGCACATGTGTGTGTTGGGAAACACTACCATCCTTGCTGAGTTTGCCACTGATGATGAAGTCAGCCGC TTTCTGGCACAAGCTCAGCCCCCTACACCTGCAGCAACCCCAAGTGCGCCAGCTGCGGGGTGGCAGTCG CTGGAGACCGGCCAGAACCAGTCAGATCCCGTGGGACCTGCTCTGAATCTTTTTGGTGGGTCCACTGGG CTCGGGCAGTGGAGCAGCAGCGCTGGTGGCAGCAGCGGGGCCGATCTTGCTGGCGCTTCATTGTGGGGG CCCCCAAACTATTCTTCTAGCTTATGGGGAGTCCCAACGGTGGAAGATCCCCATAGGATGGGCAGCCCT GCTCCTTTACTACCTGGTGACCTTCTGGGAGGAGGGTCGGATTCAATCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001162501
Insert Size	5502 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001162501.1
RefSeq Size	18297 bp
RefSeq ORF	5502 bp
Locus ID	23112
UniProt ID	Q9UPQ9
MW	194 kDa
Gene Summary	Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent translational repression and siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. As scaffoldng protein associates with argonaute proteins bound to partially complementary mRNAs and simultaneously can recruit CCR4-NOT and PAN deadenylase complexes.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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