SCUBE2 (NM_001170690) Human Untagged Clone
CAT＃: SC329143
SCUBE2 (untagged)-Human signal peptide CUB domain EGF-like 2 (SCUBE2) transcript variant 2
CNY 7220.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CEGB1; CEGF1; CEGP1; scube/You |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC329143 representing NM_001170690.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGGGGTCGCGGGCCGCAACCGTCCCGGGGCGGCCTGGGCGGTGCTGCTGCTGCTGCTGCTGCTGCCG CCACTGCTGCTGCTGGCGGGGGCCGTCCCGCCGGGTCGGGGCCGTGCCGCGGGGCCGCAGGAGGATGTA GATGAGTGTGCCCAAGGGCTAGATGACTGCCATGCCGACGCCCTGTGTCAGAACACACCCACCTCCTAC AAGTGCTCCTGCAAGCCTGGCTACCAAGGGGAAGGCAGGCAGTGTGAGGACATCGATGAATGTGGAAAT GAGCTCAATGGAGGCTGTGTCCATGACTGTTTGAATATTCCAGGCAATTATCGTTGCACTTGTTTTGAT GGCTTCATGTTGGCTCATGACGGTCATAATTGTCTTGATGTGGACGAGTGCCTGGAGAACAATGGCGGC TGCCAGCATACCTGTGTCAACGTCATGGGGAGCTATGAGTGCTGCTGCAAGGAGGGGTTTTTCCTGAGT GACAATCAGCACACCTGCATTCACCGCTCGGAAGAGGGCCTGAGCTGCATGAATAAGGATCACGGCTGT AGTCACATCTGCAAGGAGGCCCCAAGGGGCAGCGTCGCCTGTGAGTGCAGGCCTGGTTTTGAGCTGGCC AAGAACCAGAGAGACTGCATCTTGACCTGTAACCATGGGAACGGTGGGTGCCAGCACTCCTGTGACGAT ACAGCCGATGGCCCAGAGTGCAGCTGCCATCCACAGTACAAGATGCACACAGATGGGAGGAGCTGCCTT GAGCGAGAGGACACTGTCCTGGAGGTGACAGAGAGCAACACCACATCAGTGGTGGATGGGGATAAACGG GTGAAACGGCGGCTGCTCATGGAAACGTGTGCTGTCAACAATGGAGGCTGTGACCGCACCTGTAAGGAT ACTTCGACAGGTGTCCACTGCAGTTGTCCTGTTGGATTCACTCTCCAGTTGGATGGGAAGACATGTAAA GATATTGATGAGTGCCAGACCCGCAATGGAGGTTGTGATCATTTCTGCAAAAACATCGTGGGCAGTTTT GACTGCGGCTGCAAGAAAGGATTTAAATTATTAACAGATGAGAAGTCTTGCCAAGATGTGGATGAGTGC TCTTTGGATAGGACCTGTGACCACAGCTGCATCAACCACCCTGGCACATTTGCTTGTGCTTGCAACCGA GGGTACACCCTGTATGGCTTCACCCACTGTGGAGACACCAATGAGTGCAGCATCAACAACGGAGGCTGT CAGCAGGTCTGTGTGAACACAGTGGGCAGCTATGAATGCCAGTGCCACCCTGGGTACAAGCTCCACTGG AATAAAAAAGACTGTGTGGCTTCTTGTGACCTGAGCTGCATCGTAAAGCGAACCGAGAAGCGGCTCCGT AAAGCCATCCGCACGCTCAGAAAGGCCGTCCACAGGGAGCAGTTTCACCTCCAGCTCTCAGGCATGAAC CTCGACGTGGCTAAAAAGCCTCCCAGAACATCTGAACGCCAGGCAGAGTCCTGTGGAGTGGGCCAGGGT CATGCAGAAAACCAATGTGTCAGTTGCAGGGCTGGGACCTATTATGATGGAGCACGAGAACGCTGCATT TTATGTCCAAATGGAACCTTCCAAAATGAGGAAGGACAAATGACTTGTGAACCATGCCCAAGACCAGGA AATTCTGGGGCCCTGAAGACCCCAGAAGCTTGGAATATGTCTGAATGTGGAGGTCTGTGTCAACCTGGT GAATATTCTGCAGATGGCTTTGCACCTTGCCAGCTCTGTGCCCTGGGCACGTTCCAGCCTGAAGCTGGT CGAACTTCCTGCTTCCCCTGTGGAGGAGGCCTTGCCACCAAACATCAGGGAGCTACTTCCTTTCAGGAC TGTGAAACCAGAGTTCAATGTTCACCTGGACATTTCTACAACACCACCACTCACCGATGTATTCGTTGC CCAGTGGGAACATACCAGCCTGAATTTGGAAAAAATAATTGTGTTTCTTGCCCAGGAAATACTACGACT GACTTTGATGGCTCCACAAACATAACCCAGTGTAAAACTTCATCCAATTCTGTGACAACATATGAAACC TGCCAGACCTACGAACGCCCCATCGCCTTCACCTCCAGGTCAAAGAAGCTGTGGATTCAGTTCAAGTCC AATGAAGGGAACAGCGCTAGAGGGTTCCAGGTCCCATACGTGACATATGATGAGGACTACCAGGAACTC ATTGAAGACATAGTTCGAGATGGCAGGCTCTATGCATCTGAGAACCATCAGGAAATACTTAAGGATAAG AAACTTATCAAGGCTCTGTTTGATGTCCTGGCCCATCCCCAGAACTATTTCAAGTACACAGCCCAGGAG TCCCGAGAGATGTTTCCAAGATCGTTCATCCGATTGCTACGTTCCAAAGTGTCCAGGTTTTTGAGACCT TACAAATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001170690 |
| Insert Size | 2424 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001170690.1 |
| RefSeq Size | 4058 bp |
| RefSeq ORF | 2424 bp |
| Locus ID | 57758 |
| UniProt ID | Q9NQ36 |
| Protein Families | Transmembrane |
| MW | 88.9 kDa |
| Gene Summary | Lipid-binding protein required for SHH long-range signaling by binding to the dually lipid-modified SHH (ShhNp) and by promoting ShhNp mobilization, solubilization and release from the cell membrane (PubMed:22902404, PubMed:22677548). Acts by enhancing the proteolytic processing (shedding) of the lipid-modified N- and C- terminal of ShhNp at the cell surface (PubMed:24522195). Synergizes with DISP1 to increase SHH secretion (PubMed:22902404). Probable cell surface coreceptor for VEGFR2 involved in VEGFR2-mediated angiogenesis (PubMed:27834687).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is shorter than isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230505 | SCUBE2 (Myc-DDK-tagged)-Human signal peptide, CUB domain, EGF-like 2 (SCUBE2), transcript variant 2 |
CNY 5912.00 |
|
| RC230505L3 | Lenti-ORF clone of SCUBE2 (Myc-DDK-tagged)-Human signal peptide, CUB domain, EGF-like 2 (SCUBE2), transcript variant 2 |
CNY 5890.00 |
|
| RC230505L4 | Lenti-ORF clone of SCUBE2 (mGFP-tagged)-Human signal peptide, CUB domain, EGF-like 2 (SCUBE2), transcript variant 2 |
CNY 5890.00 |
|
| RG230505 | SCUBE2 (tGFP-tagged) - Human signal peptide, CUB domain, EGF-like 2 (SCUBE2), transcript variant 2 |
CNY 4370.00 |
