Ataxin 7 (ATXN7) (NM_001177387) Human Untagged Clone
CAT＃: SC329227
ATXN7 (untagged)-Human ataxin 7 (ATXN7) transcript variant SCA7b
CNY 18240.00
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Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ADCAII; OPCA3; SCA7; SGF73 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_001177387, the custom clone sequence may differ by one or more nucleotides
ATGTCGGAGCGGGCCGCGGATGACGTCAGGGGGGAGCCGCGCCGCGCGGCGGCGGCGGCG GGCGGAGCAGCGGCCGCGGCCGCCCGGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCCG CCGCCTCCGCAGCCCCAGCGGCAGCAGCACCCGCCACCGCCGCCACGGCGCACACGGCCG GAGGACGGCGGGCCCGGCGCCGCCTCCACCTCGGCCGCCGCAATGGCGACGGTCGGGGAG CGCAGGCCTCTGCCCAGTCCTGAAGTGATGCTGGGACAGTCGTGGAATCTGTGGGTTGAG GCTTCCAAACTTCCTGGGAAGGACGGGACAGAATTGGACGAAAGTTTCAAGGAGTTTGGG AAAAACCGCGAAGTCATGGGGCTCTGTCGGGAAGACATGCCAATATTTGGTTTCTGTCCA GCCCATGATGATTTCTACTTGGTGGTGTGTAACGACTGTAATCAGGTTGTCAAACCGCAG GCATTTCAATCACATTATGAAAGAAGACATAGCTCATCCAGCAAGCCGCCTTTGGCCGTT CCTCCCACTTCAGTATTTTCCTTCTTCCCTTCTCTGTCCAAAAGCAAAGGAGGCAGTGCA AGTGGAAGCAACCGTTCTTCCAGTGGAGGTGTTCTTAGCGCATCCTCATCAAGTTCCAAG TTGTTGAAATCACCCAAAGAGAAACTGCAGCTCAGGGGGAACACCAGGCCAATGCATCCC ATTCAGCAAAGTAGAGTTCCCCATGGTAGAATCATGACACCCTCTGTGAAAGTGGAAAAG ATTCATCCGAAAATGGATGGCACACTACTGAAATCTGCGGTGGGGCCAACCTGTCCTGCT ACTGTGAGTTCCTTAGTCAAGCCTGGCCTTAACTGCCCCTCAATACCAAAGCCAACCTTG CCTTCACCTGGACAGATTCTGAATGGCAAAGGGCTTCCTGCACCGCCCACTCTGGAAAAG AAACCTGAAGACAATTCCAATAATAGGAAATTTTTAAATAAGAGATTATCAGAAAGAGAG TTTGATCCTGACATCCACTGTGGGGTTATTGATCTCGACACCAAGAAGCCCTGCACCCGG TCTTTGACATGCAAGACACATTCCTTAACCCAGCGCAGGGCTGTCCAGGGTAGAAGAAAA CGATTTGATGTGTTATTAGCCGAGCACAAAAACAAAACCAGGGAAAAGGAATTGATTCGC CATCCGGACTCTCAGCAACCACCGCAGCCTCTCAGGGACCCGCATCCCGCCCCTCCTAGA ACGTCACAGGAGCCGCACCAAAACCCTCACGGAGTGATTCCTTCCGAATCAAAGCCTTTT GTAGCTAGTAAACCTAAACCTCACACCCCCAGTCTTCCAAGGCCTCCAGGCTGCCCTGCT CAGCAAGGTGGGAGTGCCCCCATTGACCCTCCTCCAGTCCATGAATCTCCACACCCTCCC CTGCCTGCCACTGAGCCAGCTTCTCGGTTATCCAGTGAGGAGGGCGAAGGCGATGACAAA GAAGAGTCTGTTGAAAAACTGGACTGTCATTATTCAGGTCATCATCCTCAGCCAGCATCT TTTTGCACATTTGGGAGCCGGCAGATAGGAAGAGGCTATTACGTGTTTGACTCCAGGTGG AATCGACTTCGCTGCGCCCTCAACCTCATGGTGGAGAAGCATCTGAATGCACAGCTATGG AAGAAAATCCCACCAGTGCCCAGTACCACCTCACCCATCTCCACACGTATTCCTCACCGG ACAAACTCTGTGCCGACATCACAATGTGGAGTCAGCTATCTGGCAGCAGCCACCGTCTCT ACATCCCCAGTCCTGCTCTCATCTACCTGCATCTCCCCAAATAGCAAATCGGTACCAGCT CATGGAACCACACTAAATGCACAGCCTGCTGCTTCAGGGGCGATGGATCCTGTGTGCAGT ATGCAATCCAGACAAGTGTCCTCTTCATCCTCATCCCCTTCCACGCCCTCTGGCCTTTCC TCGGTTCCTTCCTCCCCCATGTCCAGGAAACCTCAGAAATTGAAATCCAGCAAATCTTTG AGGCCCAAGGAGTCTTCTGGTAACAGCACTAACTGTCAAAATGCCAGTAGCAGTACCAGT GGCGGCTCAGGAAAGAAACGCAAAAACAGTTCCCCACTGTTGGTTCACTCTTCCTCCTCC TCTTCCTCCTCCTCCTCTTCTTCTCATTCCATGGAGTCTTTTAGGAAAAACTGTGTGGCT CACTCTGGGCCTCCCTACCCCTCAACGGTAACATCTTCCCATAGCATCGGCCTCAACTGT GTGACGAATAAAGCAAATGCGGTGAACGTCCGGCATGACCAGTCAGGGAGGGGCCCCCCC ACCGGGAGCCCTGCTGAATCCATCAAGAGGATGAGTGTGATGGTGAACAGCAGTGATTCT ACTCTTTCTCTTGGGCCATTCATTCACCAGTCCAATGAACTGCCTGTCAACTCCCACGGC AGTTTTTCCCACTCACACACTCCTCTAGACAAACTCATAGGAAAGAAAAGAAAGTGCTCA CCCAGCTCGAGCAGCATCAACAACAGCAGCAGCAAACCCACAAAGGTTGCCAAAGTGCCA GCCGTGAACAATGTCCACATGAAACACACAGGCACCATCCCAGGGGCACAAGGACTGATG AACAGTTCCCTCCTTCATCAGGATATCTCCTCACCTTGCTTACGAACAGGAATTTCAGCA ACATCACCCCAGAGCCCTGACTTAAAATCCAAAGGCACGTCCCTGACAGCTGAAAATAGC ACGGGGAGGAATAATGCGGACACTTTTGAGGACAAGTTACACCTCCACTCAGCACTCTGG ACTCCACGATGCCTTTGA |
| Restriction Sites | Please inquire |
| ACCN | NM_001177387 |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001177387.1, NP_001170858.1 |
| RefSeq Size | 6866 bp |
| RefSeq ORF | 2838 bp |
| Locus ID | 6314 |
| UniProt ID | O15265 |
| Gene Summary | The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the 'pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. This locus has been mapped to chromosome 3, and it has been determined that the diseased allele associated with spinocerebellar ataxia-7 contains 37-306 CAG repeats (near the N-terminus), compared to 4-35 in the normal allele. The encoded protein is a component of the SPT3/TAF9/GCN5 acetyltransferase (STAGA) and TBP-free TAF-containing (TFTC) chromatin remodeling complexes, and it thus plays a role in transcriptional regulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016] Transcript Variant: This variant (SCA7b) includes an alternate exon that causes a frameshift in the 3' coding region, compared to variant SCA7a, resulting in an isoform (b) with a distinct and longer C-terminus, compared to isoform a. The 5' UTR is incomplete in this variant due to the presence of alternate splicing choices further upstream. There are no publicly available full-length transcripts representing this variant; it is represented based on data in PMID:12533095. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230589 | ATXN7 (Myc-DDK-tagged)-Human ataxin 7 (ATXN7), transcript variant SCA7b |
CNY 6920.00 |
|
| RC230589L3 | Lenti-ORF clone of ATXN7 (Myc-DDK-tagged)-Human ataxin 7 (ATXN7), transcript variant SCA7b |
CNY 9030.00 |
|
| RC230589L4 | Lenti-ORF clone of ATXN7 (mGFP-tagged)-Human ataxin 7 (ATXN7), transcript variant SCA7b |
CNY 9030.00 |
|
| RG230589 | ATXN7 (tGFP-tagged) - Human ataxin 7 (ATXN7), transcript variant SCA7b |
CNY 7890.00 |
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