SLC35A3 (NM_001271685) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AMRS
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC330954 representing NM_001271685. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAGCTCTCGCTCGGTGTTGAGCCCTGTGGTAGGCACAGATGCACCCGACCAGCACCTGGAGCTCAAG AAGCCGCAGGAACTTAAAGAAATGGAAAGGCTGCCCTTGGCAAATGAAGATAAAACAATGTTCGCCAAC CTAAAATACGTTTCCCTGGGAATTTTGGTCTTTCAGACTACCAGTTTGGTTCTAACAATGCGTTATTCC AGAACTTTAAAAGAAGAAGGACCTCGTTATCTATCTTCTACAGCAGTGGTTGTTGCTGAACTTTTGAAG ATAATGGCCTGCATTTTATTGGTCTACAAAGACAGCAAATGTAGTCTAAGAGCACTGAATCGAGTACTA CATGATGAAATTCTTAATAAACCTATGGAAACACTTAAACTTGCTATTCCATCAGGGATCTATACTCTT CAGAATAATTTACTGTATGTGGCACTATCAAATCTAGATGCAGCTACTTATCAGGTCACGTATCAGTTA AAAATTCTTACAACAGCATTATTTTCTGTGTCTATGCTTAGTAAAAAATTGGGTGTATACCAGTGGCTG TCCCTAGTAATTTTGATGACAGGAGTTGCTTTTGTACAGTGGCCCTCAGATTCTCAGCTTGATTCTAAG GAACTTTCAGCTGGTTCTCAATTTGTAGGACTCATGGCAGTTCTCACAGCATGTTTTTCAAGTGGCTTT GCTGGGGTTTACTTTGAGAAAATCTTAAAAGAAACAAAACAATCAGTGTGGATAAGAAATATTCAGCTT GGTTTCTTTGGAAGTATATTTGGATTAATGGGTGTATACATTTATGATGGAGAACTGGTATCAAAGAAT GGATTTTTTCAGGGATATAACCGACTGACCTGGATAGTAGTTGTTCTTCAGGCACTTGGAGGCCTTGTA ATAGCTGCTGTTATTAAGTATGCAGATAATATTTTAAAAGGATTTGCAACCTCTTTATCGATAATATTA TCAACATTGATCTCCTATTTTTGGCTTCAAGATTTTGTGCCAACCAGTGTCTTTTTCCTTGGAGCCATC CTTGTAATAACAGCTACTTTTTTGTATGGTTATGATCCCAAACCTGCAGGAAATCCCACTAAAGCATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001271685
Insert Size	1104 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271685.1
RefSeq Size	5721 bp
RefSeq ORF	1104 bp
Locus ID	23443
UniProt ID	Q9Y2D2
Protein Families	Transmembrane
MW	40.7 kDa
Gene Summary	This gene encodes a UDP-N-acetylglucosamine transporter found in the golgi apparatus membrane. In cattle, a missense mutation in this gene causes complex vertebral malformation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012] Transcript Variant: This variant (3) uses an alternate 5' exon structure, which introduces an upstream start codon, compared to variant 1. It encodes isoform 3, which has a longer N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.