CGNL1 (NM_001252335) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	JACOP; PCING
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC332187 representing NM_001252335. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGCTGTATTTCGGTGAATATCAACATGTGCAGCAGGAATATGGGGTCCATCTGAGACTCGCAAGT GATGATACCCAAAAATCAAGGAGTTCCCAGAACTCCAAGGCAGGCTCCTACGGTGTCAGTATTCGGGTC CAGGGAATTGATGGTCACCCCTATATTGTCCTGAATAACACAGAACGGTGCCTAGCAGGCACATCGTTT TCTGAAAATGGGCCACCCTTTCCACCTCCAGTGATAAATAACCTGCCTCTACATTCCAGCAATGGTTCT GTGCCAAAGGAGAACAGTGAAGAACTTCAGCTTCCAGAAAACCCATACGCCCAGCCTAGCCCAATAAGA AACCTGAAACAGCCCCTGCTCCATGAGGGCAAGAATGGAGTTCTAGATCGCAAAGACGGGTCTGTGAAG CCATCTCACCTGCTGAACTTTCAGAGGCATCCAGAGCTTTTGCAACCCTATGACCCTGAAAAGAATGAG TTGAATTTACAAAATCACCAGCCTTCTGAGAGTAATTGGCTAAAAACGTTGACAGAAGAAGGCATCAAC AATAAGAAGCCTTGGACTTGCTTTCCCAAACCTAGCAATTCCCAGCCTACCAGTCCCTCCTTGGAAGAC CCGGCCAAATCTGGTGTGACAGCTATTCGTTTATGCAGCTCCGTGGTCATAGAGGACCCCAAAAAGCAG ACCTCAGTGTGTGTAAACGTTCAGAGCTGCACCAAGGAGAGGGTGGGAGAGGAGGCCCTTTTCACTAGC GGGAGGCCCCTGACTGCCCACAGCCCACATGCCCACCCTGAAACCAAGAAAACCAGGCCAGATGTTCTT CCCTTCCGGCGACAGGATTCAGCGGGACCCGTCCTGGATGGAGCTCGGTCCCGGAGGTCCTCCTCGTCA TCCACAACTCCCACGTCAGCCAACTCTTTGTACAGGTTTTTACTGGATGATCAGGAATGTGCCATCCAT GCCGACAACGTCAATCGTCATGAAAACAGAAGGTATATTCCCTTCCTGCCAGGAACTGGACGGGATATT GATACAGGATCAATTCCTGGTGTGGATCAGTTAATTGAAAAATTTGATCAAAAACCTGGGCTTCAGAGA AGAGGAAGGTCTGGGAAGCGAAACAGAATTAATACAGATGACAGGAAAAGATCCAGAAGCGTGGATAGC GCCTTTCCTTTTGGCCTCCAAGGGAACTCGGAGTACCTGATTGAATTCAGTAGGAACTTGGGCAAGTCA AGCGAACACCTCCTCCGGCCTTCCCAGGTGTGCCCGCAGCGGCCACTGTCTCAGGAGCGCCGTGGGAAA CAGAGCGTGGGCCGCACCTTTGCAAAGCTGCAGGGAGCAGCGCACGGGGCTTCATGTGCCCACTCCAGG CCTCCCCAGCCGAACATAGATGGGAAAGTTCTGGAAACCGAAGGTAGTCAGGAAAGTACAGTGATCCGT GCGCCCTCCCTTGGTGCACAGAGTAAAAAGGAGGAGGAGGTGAAAACAGCCACCGCTACGCTGATGTTA CAGAACCGGGCAACAGCAACTTCGCCTGATTCTGGTGCCAAGAAAATTTCCGTGAAGACATTTCCTTCG GCCTCAAATACTCAGGCCACACCGGATCTCTTAAAGGGCCAGCAAGAGCTCACTCAGCAAACCAATGAG GAGACAGCTAAGCAGATTCTCTACAATTACCTCAAAGAAGGAAGCACTGATAATGACGATGCTACTAAA AGGAAAGTCAACTTGGTCTTTGAGAAAATCCAGACCTTAAAGTCTCGAGCAGCTGGGAGCGCCCAAGGA AATAACCAAGCTTGTAATTCCACATCTGAAGTCAAAGATCTATTGGAACAGAAAAGCAAGTTGACCATA GAAGTGGCTGAACTTCAGAGACAGCTTCAACTGGAAGTCAAGAATCAACAGAACATTAAAGAAGAGAGA GAGAGGATGAGAGCAAACCTAGAAGAGCTCCGAAGCCAACACAACGAAAAGGTGGAGGAGAACTCCACA TTGCAGCAACGACTGGAAGAAAGTGAAGGGGAGCTCCGGAAGAATCTGGAGGAGCTATTCCAGGTGAAG ATGGAACGGGAGCAGCATCAGACTGAGATCAGGGATCTCCAGGACCAGCTCTCAGAAATGCACGATGAA CTGGACAGTGCAAAGCGATCGGAGGACAGGGAGAAGGGAGCTCTGATTGAGGAGCTCTTACAGGCAAAA CAGGATCTTCAAGATCTGCTGATTGCCAAAGAGGAGCAAGAAGACCTCTTGAGAAAGCGAGAGCGTGAA CTCACCGCCCTGAAGGGAGCCCTGAAAGAAGAGGTTTCCAGCCATGATCAGGAGATGGACAAGCTGAAG GAGCAATATGATGCTGAGTTGCAGGCCCTGAGGGAGAGTGTGGAAGAAGCAACCAAGAATGTCGAGGTC TTGGCGAGCAGGAGCAACACTTCAGAGCAAGACCAGGCGGGGACTGAAATGCGCGTGAAGCTTCTGCAG GAGGAGAATGAGAAGCTGCAGGGAAGAAGCGAAGAGCTGGAGCGGAGAGTTGCTCAGCTTCAAAGGCAG ATCGAGGACCTGAAAGGCGATGAAGCCAAGGCGAAGGAAACGCTGAAGAAGTACGAGGGAGAAATACGA CAGTTAGAGGAGGCCCTTGTGCACGCCAGAAAGGAAGAAAAAGAAGCTGTGTCAGCCAGAAGGGCCCTG GAGAATGAACTGGAGGCTGCTCAGGGAAATCTGAGTCAGACTACCCAGGAGCAGAAGCAGTTGTCTGAG AAGCTCAAAGAGGAGAGTGAGCAGAAGGAGCAGCTAAGAAGGTTGAAGAACGAGATGGAGAATGAGCGG TGGCACCTGGGCAAAACCATTGAGAAACTGCAGAAGGAGATGGCAGACATTGTTGAGGCCTCCCGTACC TCAACCCTGGAGCTCCAGAACCAGCTGGATGAGTATAAGGAGAAAAACCGCAGGGAGCTCGCAGAAATG CAAAGACAGTTGAAGGAGAAAACGCTGGAGGCAGAAAAGTCCCGACTGACAGCCATGAAAATGCAGGAT GAGATGCGTCTGATGGAGGAAGAGTTACGGGACTACCAGAGAGCTCAGGATGAAGCACTCACAAAAAGG CAGCTTCTGGAGCAGACGCTGAAGGACCTGGAGTATGAGCTGGAAGCCAAGAGTCACCTCAAAGATGAC CGCAGCAGGCTGGTCAAGCAGATGGAGGACAAGGTGTCTCAACTGGAGATGGAACTGGAAGAAGAGAGA AACAACTCAGATTTGCTGTCTGAGAGGATCAGTAGGAGCAGGGAACAGATGGAGCAGTTGAGGAATGAG CTACTTCAGGAGAGAGCTGCGAGACAAGACTTGGAGTGCGACAAGATTTCCCTGGAGAGGCAGAACAAG GACTTAAAGAGCCGGATTATCCACCTGGAAGGTTCCTACAGGTCCAGCAAAGAGGGGCTGGTTGTGCAG ATGGAGGCCAGGATCGCGGAGCTGGAGGACCGCCTGGAGAGTGAGGAGAGGGATCGGGCCAATCTTCAG CTCAGCAACCGGCGGCTGGAGCGGAAAGTGAAGGAGCTGGTGATGCAGGTGGATGATGAGCACCTGTCA TTGACTGATCAGAAGGACCAGCTGAGCTTGCGTTTGAAAGCCATGAAGCGGCAGGTGGAGGAGGCTGAG GAGGAAATCGACAGACTGGAAAGTTCTAAAAAGAAGCTGCAGAGGGAGCTGGAGGAGCAGATGGACATG AATGAGCATCTGCAGGGGCAGCTCAACTCCATGAAGAAGGACTTAAGACTGAAGAAGCTGCCGAGTAAA GTGCTGGATGACATGGATGACGACGATGACCTCAGCACGGATGGGGGAAGCCTCTATGAGGCGCCTGTG AGCTACACATTCTCCAAGGACAGCACCGTCGCCAGCCAGATCTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001252335
Insert Size	3909 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252335.1
RefSeq Size	7353 bp
RefSeq ORF	3909 bp
Locus ID	84952
UniProt ID	Q0VF96
MW	149.1 kDa
Gene Summary	This gene encodes a member of the cingulin family. The encoded protein localizes to both adherens and tight cell-cell junctions and mediates junction assembly and maintenance by regulating the activity of the small GTPases RhoA and Rac1. Heterozygous chromosomal rearrangements resulting in association of the promoter for this gene with the aromatase gene are a cause of aromatase excess syndrome. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Nov 2011] Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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