RASGEF1A (NM_001282862) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CG4853
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC336416 representing NM_001282862. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTCCTGGAGCCCCAGGAAACTATGCCCCAGACGTCCGTTGTCTTCTCCAGCATCCTTGGGCCCAGC TGTAGCGGACAGGTGCAGCCTGGCATGGGGGAGCGTGGAGGCGGGGCCGGTGGCGGCTCCGGGGACCTC ATCTTCCAAGATGGACACCTCATCTCTGGGTCCCTGGAGGCCCTGATGGAGCACCTTGTTCCCACGGTG GACTATTACCCCGATAGGACGTACATCTTCACCTTTCTCCTGAGCTCCCGGGTCTTTATGCCCCCTCAT GACCTGCTGGCCCGCGTGGGGCAGATCTGCGTGGAGCAGAAGCAGCAGCTGGAAGCCGGGCCTGAAAAG GCCAAGCTGAAGTCTTTCTCAGCCAAGATCGTGCAGCTCCTGAAGGAGTGGACCGAGGCCTTCCCCTAT GACTTCCAGGATGAGAAGGCCATGGCCGAGCTGAAAGCCATCACACACCGTGTCACCCAGTGTGATGAG GAGAATGGCACAGTGAAGAAGGCCATTGCCCAGATGACACAGAGCCTGTTGCTGTCCTTGGCTGCCCGG AGCCAGCTCCAGGAACTGCGAGAGAAGCTCCGGCCACCGGCTGTAGACAAGGGGCCCATCCTCAAGACC AAGCCACCAGCCGCCCAGAAGGACATCCTGGGCGTGTGCTGCGACCCCCTGGTGCTGGCCCAGCAGCTG ACTCACATTGAGCTGGACAGGGTCAGCAGCATTTACCCTGAGGACTTGATGCAGATCGTCAGCCACATG GACTCCTTGGACAACCACAGGTGCCGAGGGGACCTGACCAAGACCTACAGCCTGGAGGCCTATGACAAC TGGTTCAACTGCCTGAGCATGCTGGTGGCCACTGAGGTGTGCCGGGTGGTGAAGAAGAAACACCGGACC CGCATGTTGGAGTTCTTCATTGATGTGGCCCGGGAGTGCTTCAACATCGGGAACTTCAACTCCATGATG GCCATCATCTCTGGCATGAACCTCAGTCCTGTGGCAAGGCTGAAGAAAACTTGGTCCAAGGTCAAGACA GCCAAGTTTGATGTCTTGGAGCATCACATGGACCCGTCCAGCAACTTCTGCAACTACCGTACAGCCCTG CAGGGGGCCACGCAGAGGTCCCAGATGGCCAACAGCAGCCGTGAAAAGATCGTCATCCCTGTGTTCAAC CTCTTCGTTAAGGACATCTACTTCCTGCACAAAATCCATACCAACCACCTGCCCAACGGGCACATTAAC TTTAAGAAATTCTGGGAGATCTCCAGACAGATCCATGAGTTCATGACATGGACACAGGTAGAGTGTCCT TTCGAGAAGGACAAGAAGATTCAGAGTTACCTGCTCACGGCGCCCATCTACAGCGAGGAAGCTCTCTTC GTCGCCTCCTTTGAAAGTGAGGGTCCCGAGAACCACATGGAAAAAGACAGCTGGAAGACCCTCAGGACC ACCCTTCTGAACAGAGCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282862
Insert Size	1470 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282862.1
RefSeq Size	3271 bp
RefSeq ORF	1470 bp
Locus ID	221002
UniProt ID	Q8N9B8
MW	55.5 kDa
Gene Summary	Guanine nucleotide exchange factor (GEF) with specificity for RAP2A, KRAS, HRAS, and NRAS (in vitro). Plays a role in cell migration.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

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