KLHL8 (NM_001292003) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC337019 representing NM_001292003. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGCTTCAGATTCTATGAGTAGTAAACAAGCTAGGAATCACATTACAAAGGGGAAAAGGCAACAACAG CACCAGCAAATAAAGAACAGATCCTCAATTAGTGATGGTGATGGAGAAGATTCCTTTATTTTTGAAGCA AATGAAGCTTGGAAAGATTTTCATGGTTCTCTTCTTCGATTTTATGAAAATGGAGAACTCTGTGATGTC ACACTCAAGGTTGGCTCAAAGCTAATCTCTTGTCACAAGCTGGTATTGGCTTGTGTTATTCCCTACTTT AGAGCCATGTTTCTTTCTGAAATGGCTGAAGCCAAGCAAACGCTGATTGAGATTAGAGATTTTGATGGT GATGCAATAGAAGACTTGGTAAAGTTTGTCTATTCTTCACGGCTCACTTTGACTGTTGACAATGTCCAG CCTCTCTTATATGCAGCCTGTATTCTGCAGGTTGAACTGGTGGCTAGAGCTTGTTGTGAATACATGAAG TTACATTTTCATCCCTCCAATTGCCTGGCAGTAAGAGCCTTTGCAGAAAGTCACAATCGAATAGACTTA ATGGACATGGCGGATCAGTATGCCTGTGACCATTTTACTGAAGTAGTGGAGTGTGAAGACTTTGTAAGT GTATCACCGCAGCACCTCCATAAGCTTTTGTCCTCCAGTGATCTAAATATTGAAAATGAAAAGCAGGTC TATAATGCTGCCATCAAGTGGCTTCTTGCCAATCCTCAGCATCATTCCAAATGGTTGGATGAAACACTT GCACAGGTTCGCCTGCCATTGTTGCCGGTTGATTTTCTTATGGGTGTTGTGGCAAAAGAACAGATTGTC AAGCAAAATCTAAAATGTAGAGATTTACTGGATGAAGCAAGAAATTACCACCTTCACTTGAGTAGCAGA GCAGTACCTGACTTTGAATACTCCATTCGGACTACCCCAAGGAAGCATACTGCTGGTGTGCTGTTTTGT GTAGGTGGTCGAGGTGGATCTGGTGACCCCTTTCGCAGTATTGAATGCTATTCTATCAACAAAAACAGT TGGTTCTTTGGACCAGAAATGAATAGTCGAAGGCGACATGTGGGTGTAATCTCTGTGGAAGGTAAAGTG TATGCAGTAGGTGGACATGATGGAAATGAACATTTAGGGAGTATGGAGATGTTTGATCCTCTCACTAAT AAATGGATGATGAAGGCATCAATGAACACAAAGAGGCGAGGAATTGCCTTGGCTTCCTTAGGAGGCCCA ATTTATGCAATTGGAGGGTTAGATGACAATACTTGCTTCAATGATGTGGAGAGATATGACATAGAATCT GATCAGTGGAGTACAGTGGCACCAATGAATACTCCCCGTGGAGGAGTTGGCTCTGTTGCTCTAGTAAAC CATGTTTATGCAGTAGGTGGCAATGATGGAATGGCTTCTTTATCTAGCGTGGAGAGATATGATCCACAT CTGGATAAGTGGATAGAAGTTAAAGAAATGGGTCAGCGAAGAGCAGGCAATGGAGTTAGCAAGCTTCAT GGTTGCTTATACGTAGTTGGTGGTTTTGATGATAATTCTCCTCTGAGTTCAGTTGAGCGGTATGACCCC CGAAGCAACAAGTGGGATTATGTGGCAGCACTTACTACTCCCAGAGGTGGAGTGGGAATCGCAACAGTG ATGGGCAAAATCTTTGCAGTTGGTGGTCATAATGGCAATGCATACTTAAATACAGTAGAAGCGTTTGAT CCAGTGCTGAATAGGTGGGAGCTTGTTGGATCTGTGTCTCACTGCAGAGCTGGAGCAGGAGTAGCTGTG TGTTCCTGTTTAACTAGCCAAATTCGAGATGTAGGTCATGGATCCAATAATGTGGTTGACTGTATGTGA
Restriction Sites	SgfI-MluI
ACCN	NM_001292003
Insert Size	1863 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001292003.1
RefSeq Size	5680 bp
RefSeq ORF	1863 bp
Locus ID	57563
UniProt ID	Q9P2G9
MW	68.8 kDa
Gene Summary	Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for The BCR(KLHL8) ubiquitin ligase complex mediates ubiquitination and degradation of RAPSN.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Variants 1 and 2 encode the same isoform (1).

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