RAD17 (NM_001278622) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	CCYC; HRAD17; R24L; RAD17SP; RAD24
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC337168 representing NM_001278622. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGAATCAGGTAACAGACTGGGTTGACCCATCATTTGATGATTTTCTAGAGTGTAGTGGCGTCTCTACT ATTACTGCCACATCATTAGGTGTGAATAACTCAAGTCATAGAAGAAAAAATGGGCCTTCTACATTAGAA AGCAGCAGATTTCCAGCGAGAAAAAGAGGAAATCTATCTTCCTTAGAACAGATTTATGGTTTAGAAAAT TCAAAAGAATATCTGTCTGAAAATGAACCATGGGTGGATAAATATAAACCAGAAACTCAGCATGAACTT GCTGTGCATAAAAAGAAAATTGAAGAAGTCGAAACCTGGTTAAAAGCTCAAGTTTTAGAAAGGCAACCA AAACAGGGTGGATCTATTTTATTAATAACAGGTCCTCCTGGATGTGGAAAGACAACGACCTTAAAAATA CTATCAAAGGAGCATGGTATTCAAGTACAAGAGTGGATTAATCCAGTTTTACCAGACTTCCAAAAAGAT GATTTCAAGGGGATGTTTAATACTGAATCAAGCTTCCATATGTTTCCCTATCAGTCTCAGATAGCAGTT TTCAAAGAGTTTCTACTAAGAGCGACAAAGTATAACAAGTTACAAATGCTTGGAGATGATCTGAGAACT GATAAGAAGATAATTCTGGTTGAAGATTTACCTAACCAGTTTTATCGGGATTCTCATACTTTACATGAA GTTCTAAGGAAGTATGTGAGGATTGGTCGATGTCCTCTTATATTTATAATCTCGGACAGTCTCAGTGGA GATAATAATCAAAGGTTATTGTTTCCCAAAGAAATTCAGGAAGAGTGTTCTATCTCAAATATTAGTTTC AACCCTGTGGCACCAACAATTATGATGAAATTTCTTAATCGAATAGTGACTATAGAAGCTAACAAGAAT GGAGGAAAAATTACTGTCCCTGACAAAACTTCTCTAGAGTTGCTCTGTCAGGGATGTTCTGGTGATATC AGAAGTGCAATAAACAGCCTCCAGTTTTCTTCTTCAAAAGGAGAAAACAACTTACGGCCAAGGAAAAAA GGAATGTCTTTAAAATCAGATGCTGTGCTGTCAAAATCAAAACGAAGAAAAAAACCTGATAGGGTTTTT GAAAATCAAGAGGTCCAAGCTATTGGTGGCAAAGATGTTTCTCTGTTTCTCTTCAGAGCTTTGGGGAAA ATTCTATATTGTAAAAGAGCATCTTTAACAGAATTAGACTCACCTCGGTTGCCCTCTCATTTATCAGAA TATGAACGGGATACATTACTTGTTGAACCTGAGGAGGTAGTAGAAATGTCACACATGCCTGGAGACTTA TTTAATTTATATCTTCACCAAAACTACATAGATTTCTTCATGGAAATTGATGATATTGTGAGAGCCAGT GAATTTCTGAGTTTTGCAGATATCCTCAGTGGTGACTGGAATACACGCTCTTTACTCAGGGAATATAGC ACATCTATAGCTACGAGAGGTGTGATGCATTCCAACAAAGCCCGAGGATATGCTCATTGCCAAGGAGGA GGATCAAGTTTTCGACCCTTGCACAAACCTCAGTGGTTTCTAATAAATAAAAAGTATCGGGAAAATTGC CTGGCAGCAAAAGCACTTTTTCCTGACTTCTGCCTACCAGCTTTATGCCTCCAAACTCAGCTATTGCCA TACCTTGCTCTACTAACCATTCCAATGAGAAATCAAGCTCAGATTTCTTTTATCCAAGATATTGGAAGG CTCCCTCTGAAGCGACACTTTGGAAGATTGAAAATGGAAGCCCTGACTGACAGGGAACATGGAATGATA GACCCTGACAGCGGAGATGAAGCCCAGCTTAATGGAGGACATTCTGCAGAGGAATCTCTGGGTGAACCC ACTCAAGCCACTGTGCCGGAAACCTGGTCTCTTCCTTTGAGTCAGAATAGTGCCAGTGAACTGCCTGCT AGCCAGCCCCAGCCCTTTTCAGCCCAAGGAGACATGGAAGAAAACATAATAATAGAAGACTACGAGAGT GATGGGACATAG
Restriction Sites	SgfI-MluI
ACCN	NM_001278622
Insert Size	2013 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001278622.1
RefSeq Size	3067 bp
RefSeq ORF	2013 bp
Locus ID	5884
UniProt ID	O75943
Protein Families	Druggable Genome
MW	75.8 kDa
Gene Summary	The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (9) has an alternate 5' terminal exon and lacks an internal exon in the 5' UTR, when compared to variant 1. The encoded protein (isoform 1) is identical to that encoded by variants 1, 5, 6, 7 and 8.

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