DENND1C (NM_001290331) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	FAM31C
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_001290331, the custom clone sequence may differ by one or more nucleotides ATGCAGATGGTGCCTAAATTCTGCTTCCCTTTTGATGTGGAAAGGGAGCCCCCCAGCCCCGCCGTGCAGC ATTTCACCTTCGCCCTCACAGACCTTGCCGGCAACCGCAGATTTGGTTTCTGCCGCCTGCGGGCGGGTAC CCAGAGCTGTCTCTGCATCCTCAGCCACCTGCCTTGGTTCGAGGTGTTTTACAAGCTATTGAACACAGTG GGAGACCTCCTAGCCCAGGACCAAGTCACCGAGGCAGAGGAACTTCTTCAAAATCTGTTTCAGCAGTCCC TGTCTGGGCCCCAGGCCTCAGTGGGGCTTGAGCTGGGCAGCGGAGTGACGGTCTCCAGCGGGCAGGGTAT CCCCCCCCCTACCCGGGGGAATAGCAAGCCGCTTTCCTGCTTCGTGGCCCCGGACTCCGGCCGCCTGCCA TCCATCCCTGAGAACAGGAACCTAACGGAGCTGGTGGTGGCCGTGACTGACGAGAACATCGTGGGGCTGT TCGCGGCGCTCCTGGCCGAGAGAAGAGTCCTGCTCACCGCCAGCAAACTCAGCACCCTGACCTCGTGCGT CCACGCGTCCTGCGCGCTCCTGTACCCCATGCGCTGGGAGCACGTGCTGATCCCCACGCTGCCCCCACAC CTGCTGGACTACTGCTGCGCGCCCATGCCCTACCTCATTGGAGTGCACGCCAGTCTCGCCGAGAGAGTAC GAGAAAAAGCCCTGGAGGACGTCGTGGTGCTGAACGTGGACGCCAATACCTTGGAGACGACCTTTAACGA CGTGCAGGCGCTGCCTCCAGACGTGGTGTCCCTGCTGAGGCTCCGGCTCAGGAAGGTCGCCCTGGCCCCC GGGGAAGGGGTGTCCCGTCTCTTCCTCAAAGCCCAGGCCCTGCTCTTCGGGGGGTACCGCGACGCACTCG TCTGCAGCCCGGGCCAGCCAGTGACCTTCAGTGAGGAAGTCTTCTTGGCCCAGAAGCCTGGGGCACCTCT GCAGGCCTTCCACCGGCGGGCTGTGCACCTGCAGCTGTTCAAACAGTTCATCGAAGCCCGGCTGGAGAAG CTCAACAAGGGGGAGGGCTTCTCAGATCAATTCGAGCAGGAGATCACTGGCTGCGGGGCCTCCTCAGGGG CCCTTCGATCCTATCAGCTCTGGGCCGACAATCTAAAGAAAGGTGGTGGCGCCCTCCTGCACTCAGTCAA GGCCAAGACCCAACCAGCCGTCAAGAACATGTACCGCTCGGCCAAGAGTGGCTTGAAGGGGGTGCAGAGC CTTCTAATGTATAAGGATGGGGACTCTGTCCTGCAGAGGGGGGGCTCTCTGAGGGCCCCAGCCCTCCCCA GCCGCTCAGACCGCCTGCAGCAACGCCTCCCAATCACTCAGCACTTTGGAAAGAACCGGCCCCTTCGCCC CAGCAGGAGACGCCAGCTGGAAGAGGGAACTTCCGAGCCCCCAGGGGCGGGGACACCCCCACTGAGCCCT GAGGATGAGGGGTGCCCGTGGGCAGAAGAAGCTCTGGACAGCAGCTTCTTGGGGTCTGGAGAAGAACTGG ATTTGTTGAGCGAGATTCTGGACAGTCTTAGCATGGGAGCCAAGAGCGCAGGCAGCCTGAGACCGAGCCA GAGTTTAGACTGCTGTCACAGAGGAGACCTGGACAGCTGCTTCAGCCTGCCCAACATACCAAGATGGCAA CCAGACGATAAGAAACTACCAGAGCCGGAGCCCCAGCCCCTTTCCCTGCCATCCCTGCAAAATGCCTCGT CTTTGGATGCCACCAGCTCTTCAAAGGACTCCAGGTCCCAGCTGATACCCTCAGAGTCCGACCAAGAAGT CACGTCTCCATCCCAGTCCTCAACAGCTTCTGCAGACCCAAGCATCTGGGGGGACCCCAAACCCTCTCCT CTCACAGAGCCCCTAATTCTTCATCTCACCCCTTCCCACAAGGCAGCTGAAGATTCTACAGCCCAGGAAA ACCCCACTCCCTGGCTCTCCACTGCACCCACTGAGCCCAGCCCTCCAGAAAGCCCCCAAATTCTGGCCCC CACAAAGCCCAACTTTGATATAGCCTGGACGTCCCAGCCCCTTGATCCTTCCTCAGACCCCAGTTCTCTG GAGGACCCCAGAGCCCGGCCTCCCAAAGCCCTGCTGGCAGAGCGCGCTCACCTCCAGCCACGGGAGGAAC CAGGAGCCCTGAATTCCCCTGCTACACCCACCAGCAACTGTCAAAAGTCCCAGCCCAGCAGCCGGCCCAG AGTCGCTGATCTTAAGAAGTGCTTTGAGGGTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001290331
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290331.1, NP_001277260.1
RefSeq Size	2727 bp
RefSeq ORF	2274 bp
Locus ID	79958
UniProt ID	Q8IV53
Gene Summary	The protein encoded by this gene functions as a guanine nucleotide exchange factor for the early endosomal small GTPase RAB35, which regulates endosomal membrane trafficking and is involved in actin polymerization. The encoded protein activates RAB35 by promoting the exchange of RAB35-bound GDP for GTP. This gene may play a role in linking RAB35 activation with the clathrin machinery. [provided by RefSeq, May 2017] Transcript Variant: This variant (2) differs in the 5' UTR and the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform 2, which has a shorter N-terminus, compared to isoform 1.

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