Human PAN3 activation kit by CRISPRa

Specifications

Product Data
Format	3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
Symbol	PAN3
Locus ID	255967
Kit Components	GA116842G1, PAN3 gRNA vector 1 in pCas-Guide-GFP-CRISPRa GA116842G2, PAN3 gRNA vector 2 in pCas-Guide-GFP-CRISPRa GA116842G3, PAN3 gRNA vector 3 in pCas-Guide-GFP-CRISPRa 1 CRISPRa-Enhancer vector, SKU GE100056 1 CRISPRa scramble vector, SKU GE100077
Reference Data
RefSeq	NM_175854
Synonyms	OTTHUMP00000018179; OTTHUMP00000018180; OTTHUMP00000042400; PABP-dependent pol; PABP1-dependent poly A-specific ribonuclease subunit PAN3; PAN3 poly(A) specific ribonuclease subunit homolog (S. cerevisiae); PAN3 polyA specific ribonuclease subunit homolog
Summary	Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins.[UniProtKB/Swiss-Prot Function]

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