CTCF Human Gene Knockout Kit (CRISPR)

Specifications

Product Data
Format	2 gRNA vectors, 1 GFP-puro donor, 1 scramble control
Donor DNA	GFP-puro
Symbol	CTCF
Locus ID	10664
Kit Components	KN202416G1, CTCF gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: ACTTACCAGAGACGCCGGGA KN202416G2, CTCF gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: AGTTTCGGACTCCTCCACAA KN202416-D, donor DNA containing left and right homologous arms and GFP-puro functional cassette. Homologous arm and GFP-puro sequences (showhide) pUC vector backbone in gray; Left arm sequence in blue; GFP-puro in green; Right arm in violet TAACATCCTG AGTCACCAAT TTCCTGTTAA TTGGGTTGGA TCTAGAGACT TGATTGGTTT CAGAGTTTTG GAGTTTGTTT GACAAAATTG CATTATAGGT AGTGGTTTTT TCTTTTTCTT TTTTTTTTTT TTTTTTTTGA GATGGAGTCT TGCTCTGTCC CCCACGCTGG AGCGCAGTGG TGCAATCTCG GCTCACTGCA AGCTCCGCCT CCTGGATTCA AGCTATTCTT CTGCCTCAGC CTCCCGAGTA GCTGGGACTA CAGGCGCCCG CCACCATGCC TGGCTAATTT TTTTTGTATT TTTAGTAGAG ACGGGGTTTC ACCGTGTTAG CCAGGATGTT CTCGATCTCC TGACCTCGTG ATCCGCCCGC CTCAGCCTCC CAAAGTGCTG GGATTACAGG CGTGAGCCAC TGCACCTGGC CAGTAGTGGT TGTTTCTTTC ATCAAGAGGC ACATGTCTGT TGTGTCTTTT TTAATATTAA CAACCATTGA TGCCTAATTC ATTCACCAAA GGGTCTTTTT GTTTTAAAAT GTATATTTTT ATTTAGACAT GCTTTGCTTT AAATAACAAT CTGTGTTCTC CCTTAATAAA GGCAGGGGAA CAGACGGATG GGGGTGAGGT GGTCCAGGAT GTCAACAGCA GTGTACAGAT GGTGATGATG GAACAGCTGG ACCCCACCCT TCTTCAGATG AAGACTGAAG TAATGGAGGG CACAGTGGCT CCAGAAGCAG AGGCTGCTGT GGACGATACC CAGATTATAA CTTTACAGGT TGTAAATATG GAGGAACAGC CCATAAACAT AGGAGAACTT CAGCTTGTTC AAGTACCTGT TCCTGTGACT GTACCTGTTG CTACCACTTC AGTAGAAGAA CTTCAGGGGG CTTATGAAAA TGAAGTGTCT AAAGAGGGCC TTGCGGAAAG TGAACCCATG ATATGCCACA CCCTACCTTT GCCTGAAGGG TTTCAGGTGG TTAAAGTGGG GGCCAATGGA GAGGTGGAGA CACTAGAACA AGGGGAACTT CCACCCCAGG AAGATCCTAG TTGGCAAAAA GACCCAGACT ATCAGCCACC AGCCAAAAAA ACAAAGAAAA CCAAAAAGAG CAAACTGCGT TATACAGAGG AGGGCAAAGA TGTAGATGTG TCTGTCTACG ATTTTGAGGA AGAACAGCAG GAGGGTCTGC TATCAGAGGT TAATGCAGAG GE100003, scramble sequence in pCas-Guide vector
Disclaimer	These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.
Reference Data
RefSeq	NM_001191022, NM_006565, NM_001363916
Synonyms	MRD21
Summary	This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.